Maintenance of integrity and function of isolated hepatocytes during extended suspension culture of 25°C

Liver International

Volumn 23, Issue 3, 2003, Pages 201-211

  Wigg, Alan J ^a,b   Phillips, John W ^a   Berry, Michael N ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b FLINDERS MEDICAL CENTRE   (Australia)

Author keywords

Albumin synthesis; Bioartificial liver; Hypothermia; Isolated hepatocytes; Metabolism; Suspension culture; Transmission electron microscopy

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ALBUMIN; AMMONIA; BOVINE SERUM ALBUMIN; BUFFER; GLUCOSE; POTASSIUM ION; SODIUM CHLORIDE; SODIUM ION; UREA;

ANIMAL CELL; ARTICLE; ARTIFICIAL LIVER; BIOARTIFICIAL ORGAN; BIOTECHNOLOGY; CELL CULTURE; CELL DIFFERENTIATION; CELL ENERGY; CELL FUNCTION; CELL ISOLATION; CELL STRUCTURE; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; CYTOPLASM; DRUG DETOXIFICATION; HYPOTHERMIA; LIVER CELL; LIVER METABOLISM; LIVER MITOCHONDRION; MALE; NONHUMAN; OXIDATION REDUCTION POTENTIAL; PRESSURE GRADIENT; RAT; TEMPERATURE DEPENDENCE; TRANSMISSION ELECTRON MICROSCOPY; UREA CYCLE;

EID: 0142061464     PISSN: 14783223     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0676.2003.00817.x     Document Type: Article

References (37)

1. Olumide F, Eliashiv A, Kralios N, Norton L, Eiseman B. Hepatic support with hepatocyte suspensions in a permeable membrane dialyzer. Surgery 1977; 82(5): 599-606.
2. Kasai O, Oikawa I, Asakawa H, et al. Evaluation of an artificial liver support device using isolated hepatocytes. In: Atsumi K, Maekawa M, Ota K, eds. Progress in artificial organs. Cleveland: ISAO Press, 1983; 734-8.
3. Kasai S, Kakisaka A, Mito M, Terada R, Suzuki J, Tanzawa H. Studies of hybrid artificial liver. Treatment of galactosamine induced hepatic failure in dogs by a metabolic support device using hepatocytes. Jpn J Artif Organs 1987; 16: 912-5.
4. Matsumura K N, Guevara G R, Huston H, et al. Hybrid bioartificial liver in hepatic failure: preliminary clinical report. Surgery 1987; 101(1): 99-103.
5. Margulis M S, Erukhimov E A, Andreiman L A, Viksna L M. Temporary organ substitution by hemoperfusion through suspension of active donor hepatocytes in a total complex of intensive therapy in patients with acute hepatic insufficiency. Resuscitation 1989; 18(1): 85-94.
6. Kong L B, Chen S, Demetriou A, Rozga J. Matrix-induced liver cell aggregates (MILCA) for bioartificial liver use. Int J Artificial Organs 1996; 19(1): 72-8.
7. Gerlach J C, Encke J, Hole O, Muller C, Courtney J M, Neuhaus P. Hepatocyte culture between three dimensionally arranged biomatrix-coated independent artificial capillary systems and sinusoidal endothelial cell co-culture compartments. Int J Artificial Organs 1994; 17(5): 301-6.
8. De Bartolo L, Bader A. Performance of a flat membrane bioreactor utilizing porcine hepatocytes cultured in an extracellular matrix. In: Berry M N, Edwards A M, eds. The Hepatocyte Review. Dordrecht: Kluwer Academic Publishers, 2000; 585-94.
9. Nyberg S L, Shatford R A, Payne W D, Hu W S, Cerra F B. Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: an in vitro system with application to the bioartificial liver. Rat hepatocytes cultured in cylindrical collagen gels. Cytotechnology 1992; 10(3): 205-15.
10. Allen J W, Hassanein T, Bhatia S N. Advances in bioartificial liver devices. Hepatology 2001; 34(3): 447-55.
11. Berry M N, Phillips J W, Grivell M B, Efthivoulou M A, Ribstein M C. Feasibility of using cooled isolated hepatocytes in suspension in the bioartificial liver. In: Crepaldi G, Demetriou A A, Muraca M, eds. Bioartificial liver support: the critical issues. Rome: CIC Edizioni Internationale, 1997; 70-81.
12. Olinga P, Merema M, Slooff M J, Meijer D K, Groothuis G M. Influence of 48 h of cold storage in University of Wisconsin organ preservation solution on metabolic capacity of rat hepatocytes. J Hepatol 1997; 27(4): 738-43.
13. Poullain M G, Fautrel A, Guyomard C, Chesne C, Grislain L, Guillouzo A. Viability and primary culture of rat hepatocytes after hypothermic preservation: the superiority of the Leibovitz medium over the University of Wisconsin solution for cold storage. Hepatology 1992; 15(1): 97-106.
14. Sandker G W, Weert B, Merema M T, et al. Maintenance of viability and transport function after preservation of isolated rat hepatocytes in various simplified University of Wisconsin solutions. Biochem Pharmacol 1993; 46(11): 2093-6.
15. Pahernik S A, Thasler W E, Mueller-Hoecker J, Schildberg F W, Koebe H G. Hypothernic storage of pig hepatocytes: influence of different storage solutions and cell density. Cryobiology 1996; 33(5): 552-66.
16. Chen R F. Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem 1967; 242(2): 173-81.
17. Berry M N, Edwards M, Barritt G J. High yield preparation of isolated hepatocytes from rat liver. In: Burdon R H, Knippenberg P H, eds. Isolated Hepatocytes: preparation, properties and applications. Amsterdam: Elsevier, 1991; 15-58.
18. Berry M N, Friend D S. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol 1969; 43(3): 506-20.
19. Bergmeyer H U. Methods of Enzymatic Analysis, 2nd edn. New York: Academic Press, 1974.
20. Woods A, Stirling J. Electron microscopy: the preparative techniques. In: Bancroft J D B, Gamble M, eds. The Theory and Practice of Histological Techniques, 5th edn. London: Churchill Livingstone, 2002; 679-700.
21. Berry M N, Gregory R B, Grivell R, et al. Intracellular mitochondrial membrane potential as an indicator of hepatocyte energy metabolism: further evidence for thermodynamic control of metabolism. Biochimica et Biophysica Acta 1988; 936(3): 294-306.
22. Yagi T, Hardin J A, Valenzuela Y M, Miyoshi H, Gores G J, Nyberg S L. Caspase inhibition reduces apoptotic death of cryopreserved porcine hepatocytes. Hepatology 2001; 33 (6):1432-40.
23. Fry J R, Bridges J W. The metabolism of 7-ethoxycoumarin in primary maintenance cultures of adult rat hepatocytes. Naunyn-Schmiedebergs Arch Pharmacol 1980; 311(11): 85-90.
24. Donato M T, Gomez-Lechon M J, Castell J V. A micro-assay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal Biochem 1993; 213: 29-33.
25. Miners J O, Lillywhite K J, Matthews P, Jones M E, Birkett D J. Kinetic and inhibitor studies of 4-methylum-belliferone and 1-naphthol glucuronidation in human liver microsomes. Biochem Pharmacol 1988; 37(4): 665-71.
26. Nyberg S L, Hardin J A, Matos L E, Rivera D J, Misra S P, Gores G J. Cytoprotective influence of ZVAD-fmk and glycine on gel-entrapped rat hepatocytes in a bioartificial liver. Surgery 2000; 127(4): 447-55.
27. Slikker W III, Desai V G, Duhart H, Feuers R, Imam S Z. Hypothermia enhances bcl-2 expression and protects against oxidative stress-induced cell death in Chinese hamster ovary cells. Free Rad Biol Med 2001; 31(3): 405-11.
28. Bossenmeyer-Pourie C, Koziel V, Daval J L. Effects of hypothermia on hypoxia-induced apoptosis in cultured neurons from developing rat forebrain: comparison with preconditioning. Pediatric Res 2000; 47(3): 385-91.
29. Edwards A D, Yue X, Squier M V, et al. Specific inhibition of apoptosis after cerebral hypoxia-ischaemia by moderate post-insult hypothermia. Biochem Biophys Res Commun 1995; 217(3): 1193-9.
30. Maier C M, Ahern K, Cheng M L, Lee J E, Yenari M A, Steinberg G K. Optimal depth and duration of mild hypothermia in a focal model of transient cerebral ischemia: effects on neurologic outcome, infarct size, apoptosis, and inflammation. Stroke 1998; 29(10): 2171-80.
31. Hisanaga K, Kure S, Bredesen D E, Ikeda Y, Kohsaka S, Sharp F R. Apoptotic cell death of a temperature-sensitive central neuronal cell line. Brain Res 1995; 684(1): 79-86.
32. Lindquist J M, Rehnmark S. Ambient temperature regulation of apoptosis in brown adipose tissue. Erk1/2 promotes norepinephrine-dependent cell survival. J Biol Chem 1998; 273(46): 30147-56.
33. Rozga J, Demetriou A. Development and testing of a bioartificial liver. In: Berry M, Edwards A, eds. The Hepatocyte Review. Dordrecht: Kluwer Academic Publishers, 2000; 543-59.
34. Miwa Y, Ellis J, Hughes R D, Langley P G, Wendon J A, Williams R. Effect of ELAD liver support on plasma HGF and TGF-beta 1 in acute liver failure. Int J Artificial Organs 1996; 19(4): 240-4.
35. Pazzi P, Morisiani E, Muraca M, Vilei M T, Rozga J, Demetriou A. Metabolic characterization of bioartificial liver: focus on bile production and excretron. In: Crepaldi G, Demetriou A A, Muraca M, eds. Bioartificial liver support systems: the critical issues. Rome: CIC Edizioni Intenationale, 1996; 90-7.
36. Morimoto T, Matsushima M, Sowa N, Ide K, Sawanishi K. Plasma adsorption using bilirubin-adsorbent materials as a treatment for patients with hepatic failure. Artificial Organs 1989; 13(5): 447-52.
37. Bihari D, Hughes R D, Gimson E, et al. Effects of serial resin hemoperfusion in fulminant hepatic failure. Int J Artificial Organs 1983; 6(6): 299-302.