Cosmid cloning and sample sequencing of the genome of the uncultivable mollicute, Western X-disease phytoplasma, using DNA purified by pulsed-field gel electrophoresis

FEMS Microbiology Letters

Volumn 221, Issue 2, 2003, Pages 203-211

  Liefting, Lia W ^a   Kirkpatrick, Bruce C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cosmid cloning; Genome sequence; Mollicute; Plant pathogen; Uncultivable; Western X disease phytoplasma

Indexed keywords

ARTICLE; AT RICH SEQUENCE; BACTERIAL CHROMOSOME; BACTERIAL GENOME; COSMID VECTOR; DNA PURIFICATION; GENE MAPPING; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYTOPLASMA; PLANT DISEASE; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; UNINDEXED SEQUENCE; CHROMOSOME MAP; CONTIG MAPPING; COSMID; DNA SEQUENCE; GENE LIBRARY; GENE VECTOR; GENETICS; MICROBIOLOGY; MOLECULAR GENETICS; MOLLICUTES;

HEXAPODA; PHYTOPLASMA; WESTERN X PHYTOPLASMA;

CHROMOSOME MAPPING; CLONING, MOLECULAR; CONTIG MAPPING; COSMIDS; ELECTROPHORESIS, GEL, PULSED-FIELD; GENETIC VECTORS; GENOME, BACTERIAL; GENOMIC LIBRARY; MOLECULAR SEQUENCE DATA; MOLLICUTES; PLANT DISEASES; SEQUENCE ANALYSIS, DNA;

EID: 0142030491     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00183-6     Document Type: Article

References (25)

1. Razin, S. (1985) Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol. Rev. 49, 419-455.
2. Doi, Y., Teranaka, M., York, K. and Asuyama, H. (1967) Mycoplasma or PLT group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches' broom, aster yellows or Paulowinia witches' broom. Ann. Phytopathol. Soc. Jpn. 33, 259-266.
3. Jensen, D.D. (1959) A plant virus lethal to its insect vector. Virology 8, 164-175.
4. Jensen, D.D. (1971) Vector fecundity reduced by Western X-disease. J. Invertebr. Pathol. 17, 389-394.
5. Fraser, CM., Gocayne, J.D., White, O., Adams, M.D., Clayton, R.A., Fleischmann, R.D., Bult, C.J., Kerlavage, A.R., Sutton, G. and Kelley, J.M. et al. (1995) The minimal gene complement of Mycoplasma genitalium. Science 270, 397-403.
6. Himmelreich, R., Hilbert, H., Plagens, H., Pirkl, E., Li, B.-C. and Herrmann, R. (1996) Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res. 24, 4420-4449.
7. Glass, J.I., Lefkowitz, E.J., Glass, J.S., Heiner, C.R., Chen, E.T. and Cassell, G.H. (2000) The complete sequence of the mucosal pathogen Ureaplasma urealyticum. Nature 407, 757-762.
8. Chambaud, I., Heilig, R., Ferris, S., Barbe, V., Samson, D., Galisson, F., Moszer, I., Dybvig, K., Wróblewski, H., Viari, A., Rocha, E.P.C. and Blanchard, A. (2001) The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res. 29, 2145-2153.
9. Sasaki, Y., Ishikawa, J., Yamashita, A., Oshima, K., Kenri, T., Furuya, K., Yoshino, C., Horino, A., Shiba, T., Sasaki, T. and Hattori, M. (2002) The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans. Nucleic Acids Res. 30, 5293-5300.
10. Firrao, G., Smart, C.D. and Kirkpatrick, B.C. (1996) Physical map of the Western X-disease phytoplasma chromosome. J. Bacteriol. 178, 3985-3988.
11. Kirkpatrick, B.C., Stenger, D.C., Morris, T.J. and Purcell, A.H. (1987) Cloning and detection of DNA from a nonculturable plant pathogenic mycoplasma-like organism. Science 238, 197-200.
12. Ahrens, U. and Seemüller, E. (1992) Detection of DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82, 828-832.
13. Neimark, H. and Kirkpatrick, B.C. (1993) isolation and characterization of full-length chromosomes from non-culturable plant-pathogenic mycoplasma-like organisms. Mol. Microbiol. 7, 21-28.
14. Wenzel, R. and Herrmann, R. (1988) Physical mapping of the Mycoplasma pneumoniae genome. Nucleic Acids Res. 16, 8323-8336.
15. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
16. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.L. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402.
18. Lowe, T.M. and Eddy, S.R. (1997) tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 25, 955-964.
19. T. J. Bacteriol. 176, 7694-7702.
20. Glaser, P., Kunst, F., Arnaud, M., Coudart, M.-P., Gonzales, W., Hullo, M.-F., Ionescu, M., Lubochinsky, B., Marcelino, L., Moszer, I., Presecan, E., Santana, M., Schneider, E., Schweizer, J., Vertes, Á., Rapoport, G. and Danchin, A. (1993) Bacillus subtilis genome project: cloning and sequencing of the 97 kb regions from 325° to 333°. Mol. Microbiol. 10, 371-384.
21. Ogasawara, N., Nakai, S. and Yoshikawa, Y. (1994) Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin. DNA Res. 1, 1-6.
22. Frengen, E., Weichenhan, D., Zhao, B., Osoegawa, K., van Geel, M. and de Jong, P.J. (1999) A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites. Genomics 58, 250-253.
23. Blomquist, C.L., Barbara, D.J., Davies, D.L., Clark, M.F. and Kirkpatrick, B.C. (2001) An immunodominant membrane protein gene from the Western X-disease phytoplasma is distinct from those of other phytoplasmas. Microbiology 147, 571-580.
24. Yu, Y.-L., Yeh, K.-W. and Lin, C.-P. (1998) An antigenic protein gene of a phytoplasma associated with sweet potato witches' broom. Microbiology 144, 1257-1262.
25. Berg, M., Davies, D.L., Clark, M.F., Vetten, H.J., Maier, G., Marcone, C. and Seemüller, E. (1999) isolation of the gene encoding an immunodominant membrane protein of the apple proliferation phytoplasma, and expression and characterization of the gene product. Microbiology 145, 1937-1943.