Isolation of the gene encoding an immunodominant membrane protein of the apple proliferation phytoplasma, and expression and characterization of the gene product

Microbiology

Volumn 145, Issue 8, 1999, Pages 1937-1943

  Berg, Michael ^a   Davies, David L ^b   Clark, Michael F ^b   Vetten, H Joseph ^c   Maier, Gernot ^d   Marcone, Carmine ^a   Seemüller, Erich ^a  

^a Inst fur Pflanzenschutz im Obstbau   (Germany)

^b HORTICULTURE RESEARCH INTERNATIONAL   (United Kingdom)

^c INSTITUTE FOR EPIDEMIOLOGY AND PATHOGEN DIAGNOSTICS   (Germany)

^d GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Antiserum; Apple proliferation; Gene; Immunogenic membrane protein; Phytoplasma

Indexed keywords

ANTIGEN; ANTISERUM; BACTERIAL PROTEIN; DNA FRAGMENT; GENE PRODUCT; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY;

AGRICULTURE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DATA BASE; ESCHERICHIA COLI; GENE EXPRESSION; GENE ISOLATION; GENE SEQUENCE; GRAM NEGATIVE INFECTION; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOBLOTTING; MOLECULAR WEIGHT; MYCOPLASMA; NONHUMAN; OLIGONUCLEOTIDE PROBE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOUTHERN BLOTTING;

ESCHERICHIA COLI;

EID: 0032822657     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-8-1937     Document Type: Article

References (30)

1. Ahrens, U. & Seemüller, E. (1992). Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82, 828-832.
2. Barbara, D. J., Davies, D. L. & Clark, M. F. (1998). Cloning and sequencing of a major membrane protein from chlorante (aster yellows) phytoplasma. Proceedings of the 12th International Meeting of the International Organization of Mycoplasmology, Sydney, Australia, p. 183. Sydney: International Organization of Mycoplasmology.
3. Berg, M. & Seemüller, E. (1999). Chromosomal organization and nucleotide sequence of the genes coding for the elongation factors G and Tu of the apple proliferation phytoplasma. Gene 226, 103-109.
4. Bordier, C. (1981). Phase separation of integral membrane proteins in Triton X-114 solution. J Biol Chem 256, 1604-1607.
5. Chang, F. L., Chen, C. C. & Lin, C. P. (1995). Monoclonal antibody for the detection and identification of a phytoplasma associated with rice yellow dwarf. Eur J Plant Pathol 101, 511-518.
6. Clark, M. F., Morton, A. & Buss, S. L. (1989). Preparation of mycoplasma immunogens from plants and a comparison of polyclonal and monoclonal antibodies made against primula yellows MLO-associated antigens. Ann Appl Biol 114, 111-124.
7. Errampalli, D. & Fletcher, J. (1993). Production of monospecific polyclonal antibodies against aster yellows mycoplasmalike organism-associated antigen. Phytopathology 83, 1279-1282.
8. Garnier, M., Zreik, L. & Bove, J. M. (1991). Witches' broom, a lethal mycoplasmal disease of lime trees in the Sultanate of Oman and the United Arab Emirates. Plant Dis 75, 546-551.
9. Hoheisel, J. D., Lennon, G. G., Zehetner, G. & Lehrach, H. (1991). Use of high coverage reference libraries of Drosophila melanogaster for rational data analysis: a step towards mapping and sequencing of the genome. J Mol Biol 220, 903-914.
10. Holton, T. A. & Graham, M. W. (1990). A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res 19, 1156.
11. Jiang, Y. P., Lei, J. D. & Chen, T. A. (1988). Purification of aster yellows agent from diseased lettuce using affinity chromatography. Phytopathology 78, 828-831.
12. Kahane, I. & Horowitz, S. (1993). Adherence of mycoplasmas to cell surface. In Subcellular Biochemistry, vol. 20, pp. 225-241. Edited by S. Rottem & I. Kahane. New York & London : Plenum.
13. Kollar, A. & Seemüller, E. (1989). Base composition of the DNA of mycoplasmalike organisms associated with various plant diseases. J Phytopathol 127, 177-186.
14. Krause, D. C. & Taylor-Robinson, D. (1992). Mycoplasmas which infect humans. In Mycoplasmas. Molecular Biology and Pathogenesis, pp. 414-444. Edited by J. Maniloff, R. N. McElhaney, L. R. Finch & J. B. Baseman. Washington, DC: American Society for Microbiology.
15. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 136, 180-184.
16. Lee, I.-M. & Davis, R. E. (1992). Mycoplasmas which infect plants and insects. In Mycoplasmas. Molecular Biology and Pathogenesis, pp. 379-390. Edited by J. Maniloff, R. N. McElhaney, L. R. Finch & J. B. Baseman. Washington, DC: American Society for Microbiology.
17. Lin, C.-P. & Chen, T. A. (1985). Monoclonal antibodies against the aster yellows agent. Science 227, 1233-1235.
18. Lorenz, K.-H., Schneider, B., Ahrens, U. & Seemüller, E. (1995). Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopathology 85, 771-776.
19. McCoy, R. E., Caudwell, A., Chang, C. J. & 16 other authors (1989). Plant diseases associated with mycoplasma-like organisms. In The Mycoplasmas, vol. V, pp. 546-623. Edited by R. F. Whitcomb & J. G. Tully. San Diego: Academic Press.
20. Milne, R. G., Ramasso, E., Lenzi, R., Masenga, V., Sarindu, S. & Clark, M. F. (1995). Pre- and post-embedding immunogold labeling and electron microscopy in plant host tissue of three antigenically unrelated MLOs: primula yellows, tomato big bud and Bermuda grass white leaf. Eur J Plant Pathol 101, 57-67.
21. Onuki, M., Hayashi, T. & Sakai, J. (1992). Detection of antigenic proteins of mycoplasmalike organism (MLO) associated with rice yellow dwarf from infected rice plants. Ann Phytopathol Soc Jpn 58, 613.
22. Saeed, E., Rage, P. & Cousin, M. T. (1992). Determination of the antigenic protein size associated with faba bean phyllody MLO by using (SDS-PAGE) electrophoresis and immunotransfer. J Phytopathol 136, 1-8.
23. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
24. Seddas, A., Meignoz, R., Daire, X., Boudon-Padieu, E. & Caudwell, A. (1993). Purification of grapevine flavescence dorée MLO (mycoplasma-like organism) using immunoaffinity. Curr Microbiol 27, 229-236.
25. Seemüller, E., Marcone, C., Lauer, U., Ragozzino, A. & Göschl, M. (1998). Current status of molecular classification of the phytoplasmas. J Plant Pathol 80, 3-26.
26. Shine, J. & Dalgarno, L. (1974). The 3́-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to non-sense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71, 1342-1346.
27. Sinha, R. C. (1979). Purification and serology of mycoplasma-like organisms from aster yellows-infected plants. Can J Plant Pathol 1, 65-70.
28. Wieslander, A., Boyer, M. J. & Wroblewski, H. (1992). Membrane protein structure. In Mycoplasmas. Molecular Biology and Pathogenesis, pp. 93-112. Edited by J. Maniloff, R. N. McElhaney, L. R. Finch & J. B. Baseman. Washington, DC: American Society for Microbiology.
29. Wise, K. S., Yogev, D. & Rosengarten, R. (1992). Antigenic variation. In Mycoplasmas. Molecular Biology and Pathogenesis, pp. 473-489. Edited by J. Maniloff, R. N. McElhaney, L. R. Finch & J. B. Baseman. Washington, DC: American Society for Microbiology.
30. Yu, Y.-L., Yeh, K.-W. & Lin, C.-P. (1998). An antigenic protein gene of a phytoplasma associated with sweet potato witches' broom. Microbiology 144, 1257-1262.