DNA integrity determination in marine invertebrates by Fast Micromethod®

Aquatic Toxicology

Volumn 65, Issue 4, 2003, Pages 361-376

  Jakšić, Željko ^a   Batel, Renato ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

DNA integrity; Fast Micromethod ; Marine invertebrates; Mytilus galloprovincialis; PicoGreen

Indexed keywords

BLEOMYCIN IRON; DOUBLE STRANDED DNA; FLUOROCHROME; SINGLE STRANDED DNA;

ALKALINITY; ANALYTIC METHOD; ARTICLE; BIOLOGICAL MONITORING; CELL SUSPENSION; CONTROLLED STUDY; DNA DENATURATION; DNA DETERMINATION; DNA INTEGRATION; DNA ISOLATION; DNA STRAND BREAKAGE; ECOTOXICOLOGY; EXCISION REPAIR; FAST MICROMETHOD; FLUOROMETRY; GENETIC TOXICOLOGY; GENOTOXICITY; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE; TISSUE HOMOGENATE; TOXICOKINETICS;

GOSSYPIUM HIRSUTUM; HOLOTHURIA; HOLOTHURIA TUBULOSA; INVERTEBRATA; MYTILUS; MYTILUS GALLOPROVINCIALIS; SUBERITES; SUBERITES DOMUNCULA;

EID: 0142011095     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-445X(03)00150-4     Document Type: Article

References (91)

1. Accomando R., Viarengo A., Bordone R., Taningher M., Canesi L., Orunesu M. A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. Induced by genotoxic xenobiotic chemicals. Int. J. Biochem. 23:1991;227-229.
2. Adams S.M., Shepard K.L., Gredley M.S. Jr., Jimenez B.D., Ryon M.G., Shugart L.R., McCarthy J.F. The use of bioindicators for assessing the effects of pollutant stress on fish. Mar. Env. Res. 28:1989;459-464.
3. Akcha F., Izuel C., Venier P., Budzinski H., Bugerot T., Narbonne J.F. Enzymatic biomarker measurement and study of DNA adduct formation in benzo[a]pyrene-contaminated mussels, Mytilus galloprovincialis. Aquat. Toxicol. 49:2000a;287-296.
4. Akcha F., Burgeot T., Budzinski H., Pfohl-Leszkowicz A., Narbonne J.F. Induction and elimination of bulky benzo[a]pyrene-releated DNA adducts and 8-oxoGuo in mussels Mytilus galloprovincialis exposed in vivo to B[a]P-contaminated food. Mar. Ecol. Prog. Ser. 205:2000b;195-206.
5. Akcha F., Ruiz S., Zamperion C., Venier P., Burgeot T., Cadet J., Narbonne J.F. Benzo[a]pyrene-induced DNA damage in Mytilus galloprovincialis, measurement of bulky DNA adducts and DNA oxidative damage in terms of 8-oxo-7,8-dihydro-2-deoxyguanine formation. Biomarkers. 5:2000c;355-367.
6. Batel R., Vukmirović M., Bihari N., Zahn R.K., Müller W.E.G. Non-radiometric detection of DNA crosslinks in mussel hemolymph by alkaline elution. Anal. Biochem. 212:1993;402-406.
7. Batel R., Fafandel M., Blumbach B., Schröder H.C., Hassanein H.M.A., Müller I.M., Müller W.E.G. Expression of the human XPB/ERCC-3 excision repair gene-homolog in the sponge Geodia cydonium after exposure to ultraviolet radiation. Mutat. Res. 409:1998;123-133.
8. Batel R., Jakšić Ž., Bihari N., Hamer B., Fafandel M., Chauvin C., Schröder H.C., Müller W.E.G., Zahn R.K. A microplate assay for DNA damage determination (Fast Micromethod®) in cell suspensions and solid tissues. Anal. Biochem. 270:1999;195-200.
9. Bihari, N., Batel, R., Zahn, R.K., 1990a. The use of alkaline elution procedure to measure DNA damage in crab hemolymph treated with benzo[a]pyrene. In: Grandjean, E. (Ed.), World Health Organization Regional Office for Europe and United Nations Environment Programme. Carcinogenic, Mutagenic, and Teratogenic Marine Pollutants, Impact on Human Health and the Environment. Advances in Applied Biotechnology Series, vol. 5. Portfolio Publishing Company, The Woodlands, Texas, pp. 121-127.
10. Bihari N., Batel R., Zahn R.K. DNA damage determination by the alkaline elution technique in the hemolymph of mussel Mytilus galloprovincialis treated with benzo[a]pyrene and 4-nitroquinoline-N-oxide. Aquat. Toxicol. 18:1990b;13-22.
11. Bihari N., Batel R., Zahn R.K. Fractionation of DNA from marine invertebrate (Maja crispata, Mytilus galloprovincialis) hemolymph by alkaline elution. Comp. Biochem. Physiol. B102:1992;419-424.
12. Bihari N., Batel R., Jakšić Ž., Müller W.E.G., Waldman P., Zahn R.K. Comparison between the comet assay and Fast Micromethod® for measuring DNA damage in HeLa cells. Croat. Chem. Acta. 75:2002;793-804.
13. Black M.C., Ferrell J.R., Horning R.C., Martin L.K. DNA strand breakage in freshwater mussels (Anodonta grandis) exposed to lead in the laboratory and field. Environ. Toxicol. Chem. 15:1996;802-808.
14. Bolognesi C., Rabboni R., Roggieri P. Genotoxicity biomarkers in M. galloprovincialis as indicators of marine pollutants. Comp. Biochem. Physiol. 113C:1996;319-323.
15. Bresler V., Bissinger V., Abelson A., Dizer H., Sturm A., Kratke R., Fishelson L., Hansen P.D. Marine molluscs and fish as a biomarkers of pollution stress in littoral regions of the Red sea, Mediterranean sea and North sea. Helg. Mar. Res. 53:1999;219-243.
16. Burgeot T., Bocquéné G., Porte C., Dimeet J., Santella R.M., Garcia de la Parra L.M., Pfhol-Leszkowicz A., Raoux C., Galani F. Bioindicators of pollutant exposure in the northwestern Mediterranean sea. Mar. Ecol. Prog. Ser. 131:1996;125-141.
17. Canova S., Degan P., Peters L.D., Livingstone D.R., Voltan R., Venier P. Tissue dose, DNA adducts, oxidative damage and CYP1A-immunopositive proteins in mussel exposed to waterborne benzo[a]pyrene. Mutat. Res. 399:1998;17-30.
18. Chauvin C., Heidenreich E., Elmendroff-Dreikorn K., Slor H., Kutzner J., Batel R., Schröder H.C. Lack of correlation between apoptosis and DNA single-strand breaks in X-irradiated human peripheral blood mononuclear cells in the course of ageing. Mech. Aging Dev. 106:1998;117-128.
19. Cosa G., Foscaneanu K.S., McLean J.R.N., McNamee J.P., Sciano J.C. Direct determination of single-to-double-stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye-DNA complexes. Chem. Commun. 8:2000;689-690.
20. Cosa G., Foscaneanu K.S., McLean J.R.N., Sciano J.C. Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution. Photochem. Photobiol. 73:2001;585-599.
21. De Flora S., Bagnasco M., Zanacchi P. Genotoxic, carcinogenic and teratogenic hazards in marine environment, with special reference to the Mediterranean Sea. Mutat. Res. 258:1991;285-320.
22. Dixon R.D., Pruski A.M., Dixon L.R.J., Jha A.N. Marine invertebrate eco-genotoxicology: a methodological overview. Mutagenesis. 17:2002;495-507.
23. 32P -postlabeling analysis of aromatic DNA aducts in fish from polluted areas Cancer Res. 47:1987;6543-6548.
24. Elmendroff-Dreikorn K., Chauvin C., Slor H., Kutzner J., Batel R., Müller W.E.G., Schröder H.C. Assessment of DNA damage and repair in human peripheral blood mononuclear cells using a novel DNA unwinding technique. Cell. Mol. Biol. 45:1999;211-218.
25. Eastman A., Barry M.A. The origins of DNA breaks, a consequence of DNA damage, DNA repair, or apoptosis? Cancer Invest. 10:1992;229-401.
26. Everaats J.M. DNA integrity as a biomarker of marine pollution, strand breaks in seastar (Asteria rubens) and dub (Limanda limanda). Mar. Poll. Bul. 31:1995;431-438.
27. Everaats J.M. DNA integrity in Asteria rubens - a biomarker reflecting the pollution of the North sea. J. Sea Res. 37:1997;123-129.
28. Everaats J.M., Sarkar A. DNA damage as a biomarker of marine pollution, strand breaks in seastar (Asteria rubens) from the North sea. Wat. Sci. Technol. 34:1996;157-162.
29. Everaats J.M., Sliederink H.M., den Besten P.J., Halbrook R.S., Shugart L.R. Molecular responses as indicators of marine pollution, DNA damage and enzymes induction in Limanda limanda and Asterias rubens. Env. Health Perspect. 102:1994;37-43.
30. Everaats J.M., den Basten P.J., Hillebran M.Th.J., Halbrook R.S., Shugart L.R. DNA strand breaks, cytochrome P450 dependent monoxygenase system activity and levels of chlorinated biphenyl congeners in the pyloric cacea of the seastar (Asterias rubens) from the North Sea. Ecotoxicology. 7:1998;69-80.
31. Gábelová A., Slameňová D., Ružeková L., Farkasová T., Horváthová E. measurement of DNA strand breakage and repair induced with hydrogen peroxide using single cell gel electrophoresis, alkaline DNA unwinding and alkaline elution of DNA. Neoplasma. 44:1997;380-388.
32. Gosling, E., 1992. The Mussel Mytilus: Ecology, Physiology, Genetics and Culture. Developments. Aquatic Culture and Fishers Science, vol. 25. Elsevier, Amsterdam.
33. 32P -postlabeling assay for the detection of DNA adducts - false positives and artefacts and their implication for environmental biomonitoring Aquat. Toxicol. 440:1998;293-308.
34. Hassanein H.M.A., Müller C.I., Schloser D., Kratz K.L., Senyuk O.F., Schröder H.C. Measurement of DNA damage induced by irradiation with gamma-rays form a TRIGA Mark II research reactor in human cells using Fast Micromethod®. Cell. Mol. Biol. 48:2002;385-391.
35. Haugland, R.P., 1996. Nucleic acid detection. In: Spence, M.T.Z. (Ed.), Handbook of Fluorescent Probes and Research Chemicals, 6th ed. Molecular Probes Inc., Eugene, OR, pp. 143-178.
36. Herbert A., Zahn R.K. Monitoring DNA damage in Mytilus galloprovincialis and other aquatic animals. II. Pollution effects on DNA denaturation characteristics. Angew. Zoologie. 77:1990;13-33.
37. Horváthová E., Slameňová D., Gábelová A. Use of single cell gel electrophoresis (comet assay) modifications for analysis of DNA damage. Gen. Physiol. Biophys. 18:1999;389-432.
38. Imsiecke G., Custodio M., Borojevic R., Steffen R., Moustafa M.A., Müller W.E.G. Genome size and chromosomes in marine sponges (Suberites domuncula, Geodia cydonium). Cell Biol. Internat. 19:1995;995-1000.
39. ® detection and measurement of DNA damage in mussel Mytilus galloprovincialis (Lamarck, 1819) gills. Ph.D. Thesis, University of Zagreb.
40. Kanter P.M., Schwartz H.A.S. A fluorescence enhancement assay for cellular DNA damage. Mol. Pharmacol. 22:1982;145-151.
41. Kohn K.W., Erickson L.C., Ewig G.A., Friedman C.A. Fractionation of DNA from mammalian cells by alkaline elution. Biochemistry. 15:1976;4629-4637.
42. Kurelec B., Grag A., Krča S., Chacko M., Gupta C. Natural environment surpasses polluted environment in inducing DNA damage in fish. Carcinogenesis. 10:1989;1337-1339.
43. Kurelec, B., Garg, A., Krča, S., Chacko, M., Gupta, R.C., 1990. DNA adducts in marine mussel Mytilus galloprovincialis living in polluted and unpolluted environments. In: McCarthy, J.F., Shugart, L.R. (Eds.), Biomarkers of Environmental Contamination. Lewis Publishers, Boca Raton, pp. 217-222.
44. Lacorn, M., Piechotta, G., Simat, T.J., Kammann, U., Wosnoik, W., Lang, T., Müller, W.E.G., Schröder, H.C., Jenke, H.-S., Steinhart, H., 2001. Annual cycles of apoptosis, DNA strand breaks, heat shock proteins, and metallothionein isoforms in dab (Limanda limanda): influences of natural factors and consequences for biological effect monitoring 6, 108-126.
45. Livingstone D.R., Chipman J.K., Lowe D.M., Miner C., Mitchelmore C.L., Moore M.N., Peters L.D., Pipe R.K. Development of biomarkers to detect the effects of organic pollution on aquatic invertebrates, recent molecular, genotoxic, cellular and immunological studies on common mussel (Mytilus edulis L.) and other mytilids. Int. J. Environ. Poll. 13:2000;1-6.
46. Malins D.C., Haimanot R. The etiology of cancer: hydroxyl radical-induced DNA lesions in histologically normal livers of fish from a population with liver tumors. Aquat. Toxicol. 20:1991;123-130.
47. Martineau D., Legace A., Beland P., Higgins R., Armstrong D., Shugart L.R. Pathology of stranded beluga whales Delphinapretus leucas from the St. Lawrence estuary, Quebec, Canada. J. Comp. Pathol. 9:1988;287-300.
48. McFarland V.A., Inouye L.S., Lutz C.H., Jarvis A.S., Clarke J.U., McCant D.D. Biomarkers of oxidative stress and genotoxicity in livers of field-collected brown bullhead, Americus nebulosus. Arch. Environ. Contam. Toxicol. 37:1999;236-241.
49. McKelwey-Martin V.J., Green M.H.L., Schmezer P., Pool-Zobel B.L., DeMeo M.P., Collins A. The singe cell gel electrophoresis assay (comet assay): a European review. Mutat. Res. 288:1993;47-63.
50. Mitchelmore C.L., Chipman J.K. Detection of DNA strand breaks in brown trout (Salmo trutta) hepatocytes and blood cells using the single cell gel electrophoresis (comet) assay. Aquat. Toxicol. 41:1998a;161-182.
51. Mitchelmore C.L., Chipman J.K. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat. Res. 399:1998b;135-147.
52. Mitchelmore, C.L., Brimelin, C., Chipman, J.K., Livingstone, D.R., 1998a. Evidence for cytochrome P450 catalysis and free radical involment in the production of DNA strand breaks by benzo[a]pyrene and nitroaromatics in mussel (Mytilus edulis) digestive gland 41, 193-212.
53. Mitchelmore C.L., Brimelin C., Livingstone D.R., Chipman J.K. Detection of DNA strand breaks in isolated mussel (Mytilus edulis L.) digestive gland cells using the 'comet' assay. Ecotoxicol. Environ. Saf. 41:1998b;51-58.
54. ® und ihre Anwendung. Deutche Patenteamt Muenchen, No. 19724781.4-41.
55. Müller W.E.G., Batel R., Lacorn M., Steinhart H., Simat T., Lauenroth S., Hassanein H., Schröder H.C. Accumulation of cadmium and zinc in the marine sponge Suberites domuncula and its potential consequences on single-strand breaks and on expression of heat-shock protein: a natural field study. Mar. Ecol. Prog. Ser. 167:1998;127-135.
56. Müller W.E.G., Wiens M., Batel R., Steffen R., Schröder H.C., Borojevic R., Custidio M.R. Establishment of a primary cell culture from a sponge: primmorphs from Suberites domuncula. Mar. Ecol. Prog. Ser. 178:1999;205-219.
57. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1988. Use of aquatic invertebrates in genotoxicological studies. Special Issue 399, 1-122.
58. Nacci D., Jackim E. Using the alkaline unwinding assay to detect DNA damage in laboratory and environmentally exposed cells and tissues. Mar. Environ. Res. 28:1989;333-336.
59. Nacci D.E., Nelson S., Nelson W., Jackim E. Application of the alkaline unwinding assay to detect DNA strand breaks in marine bivalves. Mar. Environ. Res. 33:1992;38-100.
60. Nacci D.E., Cayula S., Jackim E. Detection of DNA damage in individual cells from marine organisms using the single cell gel assay. Aquat. Toxicol. 35:1996;197-210.
61. Nagao M., Sugimura T. Molecular biology of the carcinogen, 4-nitroquinoline-N-oxide. Adv. Cancer Res. 23:1976;131-169.
62. Olive P.L., Wlodek D., Durand R.E., Banath J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell. Res. 198:1992;259-266.
63. Ploch S.A., Lee Y.-P., MacLean E., Di Giulio R.T. Oxidative stress in liver of brown bullhead and channel catfish following exposure to tetra-buthil hydroperoxide. Aquat. Toxicol. 46:1999;231-240.
64. Qu S.-X., Bai C.-L., Stacey N.H. Determination of bulky DNA adducts in biomonitoring of carcinogenic chemical exposures: features comparison of current techniques. Biomarkers. 2:1997;3-16.
65. Rahn R., Chang S., Holland J.M., Shugart L.R. A fluorometric-HPLC assay for quantitating the binding of benzo[a]pyrene metabolites to DNA. Biochem. Biophys. Res. Commun. 109:1982;262-268.
66. Rottman M., Schröder H.C., Gramzow M., Renneisten K., Kurelec B., Dorn A., Friese U., Müller W.E.G. Specific phosphorylation of proteins in pore complex-laminae from the sponge Geodia cydonium by the homologous aggregation factor and phorbol ester. Role of proteinase C in the phosphorylation of DNA topoisomerase II. EMBO J. 6:1987;3939-3944.
67. Rydberg B. The rate of strand separation in alkali of DNA of irradiated mammalian cells. Rad. Res. 61:1975;274-287.
68. Rydberg, B., Johansen, K.J., 1978. Estimation of DNA strand breaks in single mammalian cells. In: Hanwalt, P.C., Friedberg, E.C., Fox, C.F. (Eds.), DNA Repair Mechanisms. Academic Press, New York, pp. 465-468.
69. Rye H.S., Dabora J.M., Quesada M.A., Mathies R.A., Glazer A.N. Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal. Biochem. 208:1993;144-150.
70. Santella R.M., Grinberg-Funes R.A., Young C.D., Singh V.N., Wang L.W., Perera F.P. Cigarette smoking-related polycyclic aromatic hydrocarbon-DNA adducts in peripheral mononuclear cells. Carcinogenesis. 13:1992;2041-2045.
71. Sausville E.A., Peisach J., Horwitz S.B. Effect of chelating agents and metal ions on the degradation of DNA by bleomycin. Biochemistry. 17:1978;2740-2746.
72. Schröder H.C., Batel R., Lauenroth S., Hassanein H.M.A., Lacron M., Sinat T., Steinhart H., Müller W.E.G. Induction of DNA damage and expression of heat shock protein HSP70 by polychlorinated biphenyls in the marine sponge Suberites domuncula Olivi. J. Exp. Mar. Biol. Ecol. 233:1999;285-300.
73. Shugart L.R. 5-Methyl deoxycytidine content of DNA from bluegill sunfish Lepomis macrochirus exposed to benzo[a]pyrene. Environ. Toxicol. Chem. 9:1990;205-208.
74. Shugart, L.R., 1996. Molecular markers to toxic agents. In: Newman, M.C., Jagoe, C.H. (Eds.), Ecotoxicology: A Hierarchical Treatment. Lewis Publishers Inc., Boca Raton, pp. 133-161.
75. Shugart, L.R., 1998. Structural damage to DNA in response to toxicant exposure. In: Forbes, V.E. (Ed.), Genetics and Ecotoxicology. Taylor & Francis, Philadelphia, pp. 151-168.
76. Shugart L.R. DNA damage as a biomarker of exposure. Ecotoxicology. 9:2000;329-340.
77. Shugart, L.R., Bicham, J., Jackim, G., McMahom, G., Ridley, W., Stein, J., Steinert, S., 1992. DNA alterations. In: Huggett, R.J., Kimerle, R.A., Mehrle, Jr., P.M., Berman, H.L. (Eds.), Biomarkers: Biochemical, Physiological and Histological Markers of Antropogenic Stress. Lewis Publishers, Chelsea, MI, pp. 125-153.
78. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantisation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:1988;184-191.
79. Slameňová D., Gábelová A., Ružeková L., Chalupa I., Horváthová E., Farkasová T., Bozašková E., Štětiná R. Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations. Mutat. Res. 383:1997;243-252.
80. Steinert S.A., Streib-Montee R., Leather J.M., Chadwick D.B. DNA damage in mussels at sites in San Diego bay. Mutat. Res. 399:1998;65-85.
81. Sugg D.W., Bicham J.W., Brooks J.A., Lomakin M.D., Jaoge C.H., Dallas C.E., Smith M.H., Baker R.J., Chesser R.K. DNA damage and radiocesium in channel catfish from Chernobyl. Environ. Toxicol. Chem. 15:1996;1057-1063.
82. Theodorakis C.W., D'Surney S.J., Bicham J.W., Lyne T.B., Bradley B.P., Hawkins W.E., Farkas W.F., McCarthy J.F., Shugart L.R. Sequential expression of biomarkers in bluegill sunfish exposed to contaminated sediment. Ecotoxicology. 1:1992;45-73.
83. Theodorakis C.W., D'Surney S.J., Shugart L.R. Detection of genotoxic insult as a DNA strand breaks in fish blood cells by agarose gel electrophoresis. Environ. Toxicol. Chem. 13:1994;1023-1031.
84. Venier P., Canova S. Formation of DNA adducts in the gill tissue of Mytilus galloprovincialis treated with benzo[a]pyrene. Aquat. Toxicol. 34:1996;119-133.
85. Viarengo A., Canesi L. Mussels as biological indicators of pollution. Aquaculture. 94:1991;225-243.
86. Vukmirović M., Bihari N., Zahn R.K., Müller W.E.G., Batel R. DNA damage in marine mussel Mytilus galloprovincialis as a biomarker of environmental contamination. Mar. Ecol. Prog. Ser. 109:1994;165-171.
87. Waldman P., Pivčević B., Müller W.E.G., Zahn R.K., Kurelec B. Increased genotoxicity of acetylaminoflourene by modulators of multixenobiotic resistance mechanism: studies with the fresh water clam Corbicula fluminea. Mutat. Res. 342:1995;113-123.
88. Widdows, J., Donkin, P., 1992. Mussels and environmental contaminants: bioaccumulation and physiological aspects. In: Gosling, E. (Ed.), The Mussel Mytilus: Ecology, Physiology, Genetics and Culture. Developments. Aquaticulture and Fishers Science, vol. 25. Elsevier, Amsterdam, pp. 383-424.
89. Wilson J.T., Pascoe P.L., Parry J.M., Dixon D.R. Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytilus edulis L. (Mollusca: Pelecypoda). Mutat. Res. 399:1998;87-95.
90. Würgler F.E., Kramers P.G.N. Environmental effects of genotoxins (eco-genotoxicology). Mutagenesis. 7:1992;321-327.
91. Xu L., Zheng G.J., Lam P.K.S., Richardson B.J. Relationship between tissue concentrations of polycyclic aromatic hydrocarbons and DNA adducts in green-lipped mussels (Perna viridis). Ecotoxicology. 8:1999;73-82.