Creation of a fully functional cysteine-less variant of osmosensor and proton-osmoprotectant symporter ProP from Escherichia coli and its application to assess the transporter's membrane orientation

Biochemistry

Volumn 42, Issue 40, 2003, Pages 11815-11823

  Culham, Doreen E ^c   Hillar, Alexander ^a,b   Henderson, James ^c   Ly, Anh ^c   Vernikovska, Yaroslava I ^a,b   Racher, Kathleen I ^c,d   Boggs, Joan M ^a,b   Wood, Janet M ^a,b  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF GUELPH   (Canada)

^d Government of Canada   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; BIOLOGICAL MEMBRANES; CONFORMATIONS; SUBSTITUTION REACTIONS;

OSMOSENSORS;

ESCHERICHIA COLI;

ALANINE; AMINO ACID; COTRANSPORTER; CYSTEINE; HISTIDINE; PROLINE; REAGENT; SERINE; THIOL; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CYTOPLASM; ESCHERICHIA COLI; OSMOLALITY; OSMOSIS; PRIORITY JOURNAL; PROTEOLIPOSOME;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CARBOXYLIC ACIDS; CELL MEMBRANE; CYSTEINE; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; MESYLATES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OSMOLAR CONCENTRATION; OSMOTIC PRESSURE; PROTEIN ENGINEERING; PROTEIN TRANSPORT; PROTONS; RECOMBINANT PROTEINS; SYMPORTERS; VARIATION (GENETICS);

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0141988874     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi034939j     Document Type: Article

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