The SKHR motif is required for biological function of the VirR response regulator from Clostridium perfringens

Journal of Bacteriology

Volumn 185, Issue 20, 2003, Pages 6205-6208

  McGowan, Sheena ^a   O'Connor, Jennifer R ^a   Cheung, Jackie K ^a   Rood, Julian I ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BACTERIAL TOXIN; METHIONINE; PROTEIN HISTIDINE KINASE; REGULATOR PROTEIN;

ARTICLE; BACTERIAL VIRULENCE; CARBOXY TERMINAL SEQUENCE; CLOSTRIDIUM PERFRINGENS; DNA BINDING; DNA BINDING MOTIF; GENE CONTROL; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR CLONING; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; POSIBACTERIA;

EID: 0141960305     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.20.6205-6208.2003     Document Type: Article

References (13)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Awad, M. M., A. E. Bryant, D. L. Stevens, and J. I. Rood. 1995. Virulence studies on chromosomal α-toxin and θ-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of a-toxin in Clostridium perfringens-mediated gas gangrene. Mol. Microbiol. 15:191-202.
3. Bannam, T. L., and J. I. Rood. 1993. Clostridium perfringens-Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. Plasmid 29:223-235.
4. Ba-Thein, W., M. Lyristis, K. Ohtani, I. T. Nisbet, H. Hayashi, J. I. Rood, and T. Shimizu. 1996. The virR/virS locus regulates the transcription of genes encoding extracellular toxin production in Clostridium perfringens. J. Bacteriol. 178:2514-2520.
5. Cheung, J. K., and J. I. Rood. 2000. The VirR response regulator from Clostridium perfringens binds independently to two imperfect direct repeats located upstream of the pfoA promoter. J. Bacteriol. 182:57-66.
6. Deng, W. P., and J. A. Nickoloff. 1992. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200:81-88.
7. Lyristis, M., A. E. Bryant, J. Sloan, M. M. Awad, I. T. Nisbet, D. L. Stevens, and J. I. Rood. 1994. Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens. Mol. Microbiol. 12:761-777.
8. McGowan, S., I. S. Lucet, J. K. Cheung, M. M. Awad, J. C. Whisstock, and J. I. Rood. 2002. The FxRxHrS motif: a conserved region essential for DNA binding of the VirR response regulator from Clostridium perfringens. J. Mol. Biol. 322:997-1011.
9. Nikolskaya, A. N., and M. Y. Galperin. 2002. A novel type of conserved DNA-binding in the transcriptional regulators of the AlgR/AgrA/LytR family. Nucleic Acids Res. 30:2453-2459.
10. Rood, J. I. 1998. Virulence genes of Clostridium perfringens. Annu. Rev. Microbiol. 52:333-360.
11. Shimizu, T., W. Ba-Thein, M. Tamaki, and H. Hayashi. 1994. The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens. J. Bacteriol. 176:1616-1623.
12. Stevens, D. L., R. K. Tweten, M. M. Awad, J. I. Rood, and A. E. Bryant. 1997. Clostridial gas gangrene: evidence that α and θ toxins differentially modulate the immune response and induce acute tissue necrosis. J. Infect. Dis. 176:189-195.
13. Tweten, R. 1997. Thio-activated clostridial toxins, p. 211-221. In R. W. Titball (ed.), The clostridia: molecular biology and pathogenesis. Academic Press Limited, London, United Kingdom.