The glutathione synthetase of Schizosaccharomyces pombe is synthesized as a homodimer but retains full activity when present as a heterotetramer

Journal of Biological Chemistry

Volumn 278, Issue 41, 2003, Pages 40152-40161

  Phlippen, Nadine ^a   Hoffmann, Kurt ^a   Fischer, Rainer ^a   Wolf, Klaus ^a   Zimmermann, Martin ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; GENES; MUTAGENESIS; PROTEINS; X RAY ANALYSIS; YEAST;

CLEAVAGE SITES;

BIOCHEMISTRY;

DIMER; GLUTATHIONE SYNTHASE; HISTIDINE; METALLOPROTEINASE; PROTEIN SUBUNIT; TETRAMER;

AFFINITY CHROMATOGRAPHY; ARTICLE; ENZYME ACTIVITY; ENZYME SYNTHESIS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN STRUCTURE; SCHIZOSACCHAROMYCES POMBE; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DIMERIZATION; DNA, FUNGAL; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; GLUTATHIONE SYNTHASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY, AMINO ACID;

SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 0141960038     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M303102200     Document Type: Article

References (48)

1. Meister, A., and Anderson, M. E. (1983) Annu. Rev. Biochem. 52, 711-760
2. Anderson, M. E. (1998) Chem. Biol. Interact. 111-112, 1-14
3. Penninckx, M. (2000) Enzyme Microb. Technol. 26, 737-742
4. Mooz, E. D., and Meister, A. (1967) Biochemistry 6, 1722-1734
5. Inoue, Y., Sugiyama, K., Izawa, S., and Kimura, A. (1998) Biochim. Biophys. Acta 1395, 315-320
6. Ullmann, P., Gondet, L., Potier, S., and Bach, T. J. (1996) Eur. J. Biochem. 236, 662-669
7. Wang, C. L., and Oliver, D. J. (1996) Plant Mol. Biol. 31, 1093-1104
8. Majerus, P. W., Brauner, M. J., Smith, M. B., and Minnich, V. (1971) J. Clin. Investig. 50, 1637-1643
9. Meister, A. (1985) Methods Enzymol. 113, 393-399
10. Huang, C. S., He, W., Meister, A., and Anderson, M. E. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1232-1236
11. Nakagawa, C. W., Mutoh, N., and Hayashi, Y. (1993) Biochem. Cell Biol. 71, 447-453
12. Mutoh, N., Nakagawa, C. W., Ando, S., Tanabe, K., and Hayashi, Y. (1991) Biochem. Biophys. Res. Commun. 181, 430-436
13. Wang, C. L., and Oliver, D. J. (1997) Biochem. J. 326, 563-566
14. Al-Lahham, A., Rohde, V., Heim, P., Leuchter, R., Veeck, J., Wunderlich, C., Wolf, K., and Zimmermann, M. (1999) Yeast 15, 385-396
15. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1991) Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, New York
16. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
17. Wunderlich, C. (1999) Mutations in the Gene for Glutathione Synthetase of the Fission Yeast Schizosaccharomyces pombe and Their Consequences for the Synthesis of Phytochelatins. Ph.D. thesis, Institut für Biologie IV (Mikrobiologie), Aachen University, Aachen, Germany
18. Hochstenbach, F. (1997) Fission Yeast Handbook (www.bio.uva. nl/pombe/handbook)
19. Nasim, A., Young, P., and Johnson, B. F. (1989) Molecular Biology of the Fission Yeast, Academic Press, San Diego
20. Grill, E., Winnacker, E. L., and Zenk, M. H. (1985) Science 230, 674-676
21. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
22. Laemmli, U. K. (1970) Nature 227, 680-685
23. Jeno, P., Mini, T., Moes, S., Hintermann, E., and Horst, M. (1995) Anal. Biochem. 224, 75-82
24. Polekhina, G., Board, P. G., Gali, R. R., Rossjohn, J., and Parker, M. W. (1999) EMBO J. 18, 3204-3213
25. Maundrell, K. (1990) J. Biol. Chem. 265, 10857-10864
26. Yamaguchi, H., Kato, H., Hata, Y., Nishioka, T., Kimura, A., Oda, J., and Katsube, Y. (1993) J. Mol. Biol. 229, 1083-1100
27. Lee, J., Dawes, I. W., and Roe, J. H. (1997) J. Biol. Chem. 272, 23042-23049
28. Nok, A. J. (2001) J. Biochem. Mol. Toxicol. 15, 215-220
29. Sookkheo, B., Sinchaikul, S., Phutrakul, S., and Chen, S. T. (2000) Protein Expression Purif. 20, 142-151
30. Sabeur, K., Vo, A. T., and Ball, B. A. (2001) Reproduction 122, 139-146
31. Figueiredo, E., and Duque-Magalhaes, M. C. (1994) Biochem. J. 300, 15-19
32. Basharov, M. A. (2001) Biochemistry (Russia) 65, 1380-1384
33. Frydman, J. (2001) Annu. Review Biochem. 70, 603-647
34. Hartl, F. U., and Hayer-Hartl, M. (2002) Science 295, 1852-1858
35. Lund, P. A. (2001) Adv. Microb. Physiol. 44, 93-140
36. Vainberg, I. E., Lewis, S. A., Rommelaere, H., Ampe, C., Vandekerckhove, J., Klein, H. L., and Cowan, N. J. (1998) Cell 93, 863-873
37. Demand, J., Alberti, S., Patterson, C., and Hohfeld, J. (2001) Curr. Biol. 11, 1569-1577
38. Connell, P., Ballinger, C. A., Jiang, J., Wu, Y., Thompson, L. J., Hohfeld, J., and Patterson, C. (2001) Nat. Cell Biol. 3, 93-96
39. Fan, C., Moews, P. C., Shi, Y., Walsh, C. T., and Knox, J. R. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1172-1176
40. Ponting, C. P., and Russell, R. R. (2002) Annu. Rev. Biophys. Biomol. Struct. 31, 45-71
41. Russell, R. B., and Ponting, C. P. (1998) Curr. Opin. Struct. Biol. 8, 364-371
42. Jeltsch, A. (1999) J. Mol. Evol. 49, 161-164
43. Lindqvist, Y., and Schneider, G. (1997) Curr. Opin. Struct. Biol. 7, 422-427
44. Luger, K., Hommel, U., Herold, M., Hofsteenge, J., and Kirschner, K. (1989) Science 243, 206-210
45. Nakamura, T., and Iwakura, M. (1999) J. Biol. Chem. 274, 19041-19047
46. Iwakura, M., Nakamura, T., Yamane, C., and Maki, K. (2000) Nat. Struct. Biol. 7, 580-585
47. Cheltsov, A. V., Barber, M. J., and Ferreira, G. C. (2001) J. Biol. Chem. 276, 19141-19149
48. Jung, J., and Lee, B. (2001) Protein Sci. 10, 1881-1886