Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used

Infection and Immunity

Volumn 71, Issue 10, 2003, Pages 5750-5755

  Velikovsky, Carlos A ^a,d   Goldbaum, Fernando A ^a   Cassataro, Juliana ^a,b   Estein, Silvia ^c   Bowden, Raúl A ^c   Bruno, Laura ^a,b   Fossati, Carlos A ^a   Giambartolomei, Guillermo H ^a,b  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b HOSPITAL DE CLÍNICAS JOSÉ DE SAN MARTÍN   (Argentina)

^c UNIVERSIDAD NACIONAL DEL CENTRO DE LA PROVINCIA DE BUENOS AIRES   (Argentina)

^d UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALUMINUM HYDROXIDE; BRUCELLA VACCINE; FREUND ADJUVANT; GAMMA INTERFERON; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; INTERLEUKIN 10; INTERLEUKIN 2; INTERLEUKIN 4; LUMAZINE; LUMAZINE SYNTHASE; PHOSPHORYL LIPID A; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BRUCELLA ABORTUS; BRUCELLOSIS; CONTROLLED STUDY; CYTOKINE PRODUCTION; DRUG EFFECT; DRUG FORMULATION; FEMALE; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOGENICITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; TH1 CELL; TH2 CELL;

ADJUVANTS, IMMUNOLOGIC; ANIMALS; ANTIBODIES, BACTERIAL; BRUCELLA; BRUCELLA VACCINE; BRUCELLOSIS; FEMALE; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; MULTIENZYME COMPLEXES; TH1 CELLS; TH2 CELLS; VACCINES, SYNTHETIC;

EID: 0141780959     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.71.10.5750-5755.2003     Document Type: Article

References (38)

1. Aberle, J. H., S. W. Aberle, S. L. Allison, K. Stiasny, M. Ecker, C. W. Mandl, R. Berger, and F. X. Heinz. 1999. A DNA immunization model study with constructs expressing the tick-borne encephalitis virus envelope protein E in different physical forms. J. Immunol. 163:6756-6761.
2. Al-Mariri, A., A. Tibor, P. Mertens, X. De Bolle, P. Michel, J. Godfroid, K. Walravens, and J.-J. Letesson. 2001. Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. Infect. Immun. 69:6264-6270.
3. Araya, L. N., P. H. Elzer, G. H. Rowe, F. M. Enright, and A. J. Winter. 1989. Temporal development of protective cell-mediated and humoral immunity in BALB/c mice infected with Brucella abortus. J. Immunol. 143:3330-3337.
4. Araya, L. N., and A. J. Winter. 1990. Comparative protection of mice against virulent and attenuated strains of Brucella abortus by passive transfer of immune T cells or serum. Infect. Immun. 58:254-256.
5. Bachmann, M. F., and R. M. Zinkernagel. 1996. The influence of virus structure on antibody responses and virus serotype formation. Immunol. Today 17:553-558.
6. Baldi, P. C., C. A. Velikovsky, B. C. Braden, G. H. Giambartolomei, C. A. Fossati, and F. A. Goldbaum. 2000. Structural, functional and immunological studies on a polymeric bacterial protein. Braz. J. Med. Biol. Res. 33:741-747.
7. Baldi, P. C., G. H. Giambartolomei, F. A. Goldbaum, L. F. Abdón, C. A. Velikovsky, R. Kittelberger, and C. A. Fossati. 1996. Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with Brucella abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9. Clin. Diagn. Lab. Immunol. 3:472-476.
8. Baldi, P. C., M. M. Wanke, M. E. Loza, N. Monachesi, and C. A. Fossati. 1997. Diagnosis of canine brucellosis by detection of IgG antibodies against an 18 kDa cytoplasmic protein of Brucella spp. Vet. Microbiol. 57:273-281.
9. Bowden, R. A., A. Cloeckaert, M. Zygmunt, and G. Dubray. 1995. Outer membrane protein and rough lipopolysaccharide-specific monoclonal anti-bodies protect mice against Brucella ovis. J. Med. Microbiol. 43:344-347.
10. Braden, B. C., C. A. Velikovsky, A. A. Cauerhff, I. Polikarpov, and F. A. Goldbaum. 2000. Divergence in macromolecular assembly: X-ray crystallographic structure analysis of lumazine synthase from Brucella abortus. J. Mol. Biol. 297:1031-1036.
11. Cespedes, S., E. Andrews, H. Folch, and A. Onate. 2000. Identification and partial characterisation of a new protective antigen of Brucella abortus. J. Med. Microbiol. 49:165-170.
12. Chackerian, B., P. Lenz, D. R. Lowy, and J. T. Schiller. 2002. Determinants of autoantibody induction by conjugated papillomavirus virus-like particles. J. Immunol. 169:6120-6126.
13. Cheers, C. 1984. Pathogenesis and cellular immunity in experimental murine brucellosis. Dev. Biol. Stand. 56:237-246.
14. Cheville, N. F., R. A. Kunkle, A. E. Jensen, and M. V. Palmer. 1995. Persistence of Brucella abortus in the livers of T cell-deficient nude mice. Lab. Investig. 73:96-102.
15. Eze, M. O., L. Yuan, R. M. Crawford, C. M. Paranavitana, T. L. Hadfield, A. K. Bhattacharjee, R. L. Warren, and D. L. Hoover. 2000. Effects of opsonization and gamma interferon on growth of Brucella melitensis 16M in mouse peritoneal macrophages in vitro. Infect. Immun. 68:257-263.
16. + T cells from influenza virus-immune mice. Immunology 95:346-351.
17. Fernandes, D., and C. Baldwin. 1995. Interleukin-10 downregulates protective immunity to Brucella abortus. Infect. Immun. 63:1130-1133.
18. Goldbaum, F. A., C. A. Velikovsky, P. C. Baldi, S. Mortl, A. Bacher, and C. A. Fossati. 1999. The 18-kDa cytoplasmic protein of Brucella spp.-an antigen useful for diagnosis-is a lumazine synthase. J. Med. Microbiol. 48:833-839.
19. Goldbaum, F. A., J. Leoni, J. C. Wallach, and C. A. Fossati. 1993. Characterization of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis. J. Clin. Microbiol. 31:2141-2145.
20. Izadjoo, M. J., Y. Polotsky, M. G. Mense, A. K. Bhattacharjee, C. M. Paranavitana, T. L. Hadfield, and D. L. Hoover. 2000. Impaired control of Brucella melitensis infection in Rag1-deficient mice. Infect. Immun. 68:5314-5320.
21. Jacques, I., A. Cloeckaert, J. N. Linet, and G. Dubray. 1992. Protection conferred on mice by combinations of monoclonal antibodies directed against outer-membrane proteins or smooth lipopolysaccharide of Brucella. J. Med. Microbiol. 37:100-103.
22. Katayama, S., K. Oda, T. Ohgitani, T. Hirahara, and Y. Shimizu. 1999. Influence of antigenic forms and adjuvants on the IgG subclass antibody response to Aujeszky's disease virus in mice. Vaccine 17:2733-2739.
23. Miller, M. J., R. A. Wrightsman, and J. E. Manning. 1996. Trypanosoma cruzi: protective immunity in mice immunized with paraflagellar rod proteins is associated with a T-helper type 1 response. Exp. Parasitol, 84:156-167.
24. Murphy, E. A., J. Sathiyaseelan, M. A. Parent, B. Zou, and C. L. Baldwin. 2001. Interferon-gamma is crucial for surviving a Brucella abortus infection in both resistant C57BL/6 and susceptible BALB/c mice. Immunology 103:511-518.
25. Neuzil, K. M., J. E. Johnson, Y. W. Tang, J. P. Prieels, M. Slaoui, N. Gar, and B. S. Graham. 1997. Adjuvants influence the quantitative and qualitative immune response in BALB/c mice immunized with respiratory syncytial virus FG subunit vaccine. Vaccine 15:525-532.
26. Nicoletti, P. 1990. Vaccination, p. 284-299. In K. Nielsen, and J. R. Duncan (ed.), Animal brucellosis. CRC Press, Boca Raton, Fla.
27. Nieba, L., and M. F. Bachmann. 2000. A new generation of vaccines. Mod. Asp. Immunobiol. 1:36-39.
28. Oliveira, S. C., and G. A. Splitter. 1996. Immunization of mice with recombinant L7/12 ribosomal protein confers protection against Brucella abortus infection. Vaccine 14:959-962.
29. Pasquali, P., R. Adone, L. C. Gasbarre, C. Pistoia, and F. Ciuchini. 2001. Mouse cytokine profiles associated with Brucella abortus RB51 vaccination or B. abortus 2308 infection. Infect. Immun. 69:6541-6544.
30. Schurig, G. G., N. Sriranganathan, and M. J. Corbel. 2002. Brucellosis vaccines: past, present and future. Vet. Microbiol. 90:479-496.
31. Schurig, G. G., R. M. Roop II, T. Bagchi, S. Boyle, D. Buhrman, and N. Sriranganathan. 1991. Biological properties of RB51; a stable rough strain of Brucella abortus. Vet. Microbiol. 28:171-188.
32. Sulzer, B., and A. S. Perelson. 1997. Immunons revisited: binding of multivalent antigens to B cells. Mol. Immunol. 34:63-74.
33. Tabatabai, L. B., and G. W. Pugh, Jr. 1992. Modulation of immune responses in BALB/c mice vaccinated with Brucella abortus Cu-Zn superoxide dismutase synthetic peptide vaccine. Vaccine 12:919-924.
34. Velikovsky, C. A., J. Cassataro, G. H. Giambartolomei, F. A. Goldbaum, S. Estein, R. A. Bowden, L. Bruno, C. A. Fossati, and M. Spitz. 2002. A DNA vaccine encoding lumazine synthase from Brucella abortus induces protective immunity in BALB/c mice. Infect. Immun. 70:2507-2511.
35. Victoratos, P., M. Yiangou, N. Avramidis, and I. Hadjipetrou. 1997. Regulation of cytokine gene expression by adjuvants in vivo. Clin. Exp. Immunol. 109:569-578.
36. Yip, H. C., A. Y. Karulin, M. Tary-Lehmann, M. D. Hesse, H. Radeke, P. S. Heeger, R. P. Trezza, F. P. Heinzel, T. Forsthuber, and P. V. Lehmann. 1999. Adjuvant-guided type-1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response. J. Immunol. 162:3942-3949.
37. Young, E. J. 1983. Human brucellosis. Rev. Infect. Dis. 5:821-842.
38. Zhan, Y., and C. Cheers. 1993. Endogenous gamma interferon mediates resistance to Brucella abortus infection. Infect. Immun. 61:4899-4901.