High-speed genotyping of CYP1A2*1F mutation with fluorescent hybridization probes using the LightCycler™

Pharmacogenomics

Volumn 4, Issue 5, 2003, Pages 643-646

  Popp, Johannes ^a   Messner, Barbara ^a   Steimer, Werner ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

CYP1A2; CYP1A2*1F; Genotyping; Melting curve analysis; Polymorphisms; Psychoactive drugs; Real time PCR

Indexed keywords

CYTOCHROME P450 1A2; GENOMIC DNA; RESTRICTION ENDONUCLEASE;

ALLELE; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; DRUG CLEARANCE; DRUG METABOLISM; FLUORESCENCE IN SITU HYBRIDIZATION; GENE SEQUENCE; GENETIC POLYMORPHISM; GENOTYPE; HETEROZYGOTE; HOMOZYGOSITY; HUMAN; MAJOR CLINICAL STUDY; MOLECULAR PROBE; MUTATIONAL ANALYSIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RELIABILITY;

ALLELES; CYTOCHROME P-450 CYP1A2; DNA PROBES; GENOTYPE; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; REPRODUCIBILITY OF RESULTS;

EID: 0141739767     PISSN: 14622416     EISSN: None     Source Type: Journal    
DOI: 10.1517/phgs.4.5.643.23798     Document Type: Article

References (14)

1. McManus ME, Burgess WM, Veronese ME, Huggett A, Quattrochi LC, Tukey RH: Metabolism of 2-acetylaminofluorene and benzo(a)pyrene and activation of food-derived heterocyclic amine mutagens by human cytochromes P450. Cancer Res. 50, 3367-3376 (1990).
2. Butler MA, Iwasaki M, Guengerich FP, Kadlubar FF: Human cytochrome P450PA (P450IA2), the phenacetin O-deethylase, is primarily responsible for the hepatic 3-demethylation of caffeine and N-oxidation ofcarcinogenic arylamines. Proc. Natl. Acad. Sci. USA 86, 7696-7700 (1989).
3. Kalow W, Tang BK: Caffeine as a metabolic probe: exploration of the enzyme-inducing effect of cigarette smoking. Clin. Pharmacol. Ther. 49, 44-48 (1991).
4. Nakajima M, Yokoi T, Mizutani M, Shin S, Kadlubar FF, Kamataki T: Phenotyping of CYP1A2 in Japanese population by analysis of caffeine urinary metabolites: absence of mutation prescribing the phenotype in the CYP1A2 gene. Cancer Epidemiol. Biomarkers Prev. 3, 413-421 (1994).
5. Nakajima M, Yokoi T, Mizutani M, Kinoshita M, Funayama M, Kamataki T: Genetic polymorphism in the 5′-flanking region of human CYP1A2 gene: effect on the CYP1A2 inducibility in humans. J. Biochem. (Tokyo) 125, 803-808 (1999).
6. Chida M, Yokoi T, Fukui T, Kinoshita M, Yokota J, Kamataki T: Detection of three genetic polymorphisms in the 5′-flanking region and intron 1 of human CYP1A2 in the Japanese population. Jpn. J. Cancer Res. 90, 899-902 (1999).
7. Sachse C, Brockmoller J, Bauer S, Roots I: Functional significance of a C→A polymorphism in intron 1 of the cytochrome P450 CYP1A2 gene tested with caffeine. Br. J. Clin. Pharmacol. 47, 445-449 (1999).
8. Chevalier D, Cauffiez C, Allorge D et al.: Five novel natural allelic variants-951A > C, 1042G > A (D348N), 1156A > T (I386F , 1217G > A (C406Y) and 1291C > T (C431Y)-of the human CYP1A2 gene in a French Caucasian population. Hum. Mutat. 17, 55-56 (2001).
9. Huang JD, Guo WC, Lai MD, Guo YL, Lambert GH: Detection of a novel cytochrome P450 1A2 polymorphism (F21L) in Chinese. Drug Metab. Dispos. 27, 98-101 (1999).
10. Lang NP, Butler MA, Massengill J: Rapid metabolic phenotypes for acetyltransferase and cytochrome P4501A2 and putative exposure to food-borne heterocyclic amines increase the risk for colorectal cancer or polypes. Cancer Epidemiol. Biomarkers Prev. 3, 675-682 (1994).
11. Oezdemir V, Kalow W, Okey AB et al.:Treatment-resistance to clozapine in association with ultrarapid CYP1A2 activity and the C→A polymorphism in intron 1 of the CYP1A2 gene: effect of grapefruit juice and low-dose fluvoxamine. J. Clin. Psychopharmacol. 21, 603-607 (2001).
12. Van Der Weide J, Steijns LS, Van Weelden MJ: The effect of smoking and cytochrome P450 CYP1A2 genetic polymorphism on clozapine clearance and dose requirement. Pharmacogenet. 13, 169-172 (2003).
13. Schutz E, von Ahsen N: Spreadsheet software for thermodynamic melting point prediction of oligonucleotide hybridisation with and without mismatches. Biotechnique 27, 1224 (1999).
14. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J. Mol. Biol. 215, 403-410 (1990).