Precursor-directed polyketide biosynthesis in Escherichia coli

Bioorganic and Medicinal Chemistry Letters

Volumn 13, Issue 21, 2003, Pages 3701-3704

  Kinoshita, Kenji ^a   Pfeifer, Blaine A ^b   Khosla, Chaitan ^b   Cane, David E ^a  

^a BROWN UNIVERSITY   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MACROLIDE; POLYKETIDE;

ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; DRUG IDENTIFICATION; DRUG SYNTHESIS; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN;

ESCHERICHIA COLI;

EID: 0141656418     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2003.08.008     Document Type: Article

References (17)

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13. 9) with module 2 replacing DEBS1. The construction of module 2 has been described previously: Tsuji S., Cane D.E., Khosla C. Biochemistry. 40:2001;2326.
14. 14C]propionate (56 mCi/mmol) and either diketides 2 or 3 or triketide analogues 6 and 7 were each administered at final concentrations of 1 mM. The culture was then shaken for an additional 24 h at 20°C. At this point the culture was centrifuged and 1 mL of the supernatant was extracted with 2 mL of ethyl acetate. The extract was dried in vacuo and subjected to thin-layer chromatography (TLC) analysis. Negative controls included cultures without substrate.
15. 13C) and 2D NMR and by high-resolution mass spectrometry to confirm their individual structures.
16. +: 442.3095, Found: 442.3100.
17. Kinoshita, K.; Khosla, C.; Cane, D. E. Helv. Chim. Acta Submitted for publication.