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Volumn 13, Issue 21, 2003, Pages 3689-3692

Styrylheterocycles: A novel class of inhibitors on lipopolysaccharide-Induced nitric oxide production

Author keywords

[No Author keywords available]

Indexed keywords

INDUCIBLE NITRIC OXIDE SYNTHASE; LIPOPOLYSACCHARIDE; MESSENGER RNA; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE INHIBITOR; RESVERATROL; STYRYLHETEROCYCLE DERIVATIVE; UNCLASSIFIED DRUG;

EID: 0141656270     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2003.08.013     Document Type: Article
Times cited : (12)

References (29)
  • 27
    • 85031056102 scopus 로고    scopus 로고
    • 50 values.
  • 28
  • 29
    • 85031065177 scopus 로고    scopus 로고
    • 2, 0.16 mM dNTP, 5.0 units of Taq DNA polymerase (Qiagen, Valencia, CA, USA) and 20 pmol of sense and antisense primers in 10 mM Tris-HCl (pH 8.3). The sense and antisense primers for iNOS were 5′-ATGTCCGAAGCAAACATCAC-3′ and 5′ -TAATGTCCAGGAAGTAGGTG-3′, respectively. The sense and antisense primers for β-actin were 5′-TGTGATGGTGGGAATGGGTCAG-3′ and 5′-TTTGATGTCACGCACGATTTCC-3′, respectively. The PCR amplification was performed under the following conditions: 25 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s and extension at 72°C for 30 s, using a thermal cycler (GeneAmp PCR Systems 2400; PE Applied Biosystems, USA). The amplified PCR products were separated on a 2% agarose gel and visualized by SYBR Gold staining. The bands in the gel were photographed, and the fluorescence intensities analyzed using an Alpha Imager™ (Alpha Innotech Corp., USA).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.