Detection of verotoxin-producing Escherichia coli O157:H7 by multiplex polymerase chain reaction

Microbiology and Immunology

Volumn 42, Issue 5, 1998, Pages 371-376

  Nagano, Isao ^a   Kunishima, Miyuki ^b   Itoh, Yoshihiro ^a   Wu, Zhiliang ^a   Takahashi, Yuzo ^a  

^a GIFU UNIVERSITY   (Japan)

^b Gifu Prefectural Institute of Health and Environmental Sciences   (Japan)

Author keywords

Escherichia coli O157:H7; fliC gene; Multiplex PCR; rfbE gene

Indexed keywords

BACTERIAL DNA; FLAGELLIN; VEROTOXIN;

ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI O157; HEMOLYTIC UREMIC SYNDROME; HEMORRHAGIC COLITIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031749547     PISSN: 03855600     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1348-0421.1998.tb02297.x     Document Type: Article

References (19)

1. Bilge, S.S., Vary, J.C., Jr., Dowell, S.F., and Tarr, P.I. 1996. Role of the Escherichia call O157:H7 O side chain in adherence and analysis of an rfb locus. Infect. Immun. 64: 4795-4801.
2. Fratamico, P.M., Sackitey, S.K., Wiedmann, M., and Deng, M.Y. 1995. Detection of Escherichia coli O157:H7 by multiplex PCR. J. Clin. Microbiol. 33: 2188-2191.
3. Gannon, V.P.J., D'Souza, S., Graham, T., King, R.K., Rahn, K., and Read, S. 1997. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J. Clin. Microbiol. 35: 656-662.
4. Hirata, K., Inoue, H., Mitsuno, T., Amano, T., Nakazawa, M., Yamasaki, S., Lin, Z., and Takeda, Y. 1992. Isolation of vero-cytotoxin-producing Escherichia coli from cattle and serotyping and toxin-typing of the isolated strains. J. Jpn. Assoc. Infect. Dis. 66: 950-955 (in Japanese).
5. Johnson, W.M., Lior, H., and Bezanson, G.S. 1983. Cytotoxic Escherichia coli O157:H7 associated with hemorrhagic colitis in Canada. Lancet i: 76.
6. Karch, H., and Meyer, T. 1989. Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction. J. Clin. Microbiol. 27: 2751-2757.
7. Karmali, M.A. 1989. Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2: 15-38.
8. Karmali, M.A., Petric, M., Lim, C., Fleming, P.C., Arbus, G.S., and Lior, H. 1985. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J. Infect. Dis. 151: 775-782.
9. Lawn, A.M., Orskov, I., and Orskov, F. 1977. Morphological distinction between different H serotypes of Escherichia coli. J. Gen. Microbiol. 101: 111-119.
10. Lin, Z., Kurazono, H., Yamasaki, S., and Takeda, Y. 1993. Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol. Immunol. 37: 543-548.
11. Marques, L.R.M., Peiris, J.S.M., Cryz, J.S., and O'Brien, A.D. 1987. Escherichia coli strains isolated from pigs with edema disease produce a variant of Shiga-like toxin II. FEMS Microbiol. Lett. 44: 33-38.
12. Meng, J., Zhao, S., Doyle, M.P., Mitchell, S.E., and Kresovich, S. 1997. A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7. Lett. Appl. Microbiol. 24: 172-176.
13. National Institute of Health. 1996. Verotoxin-producing strains of Escherichia coli: Infect. Agents Surveillance Rep. 17: 1-2 (in Japanese).
14. Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B., and Erlich, H.A. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491.
15. Schoenhals, G., and Whitfield, C. 1993. Comparative analysis of flagellin sequences from Escherichia coli strains possessing serologically distinct flagellar filaments with a shared complex surface pattern. J. Bacteriol. 175: 5395-5402.
16. Scotland, S.M., Rowe, B., Smith, H.R., Willshaw, G.A., and Gross, R.J. 1988. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J. Med. Microbiol. 25: 237-243.
17. Smith, N.H., Beltran, P., and Selander, R.K. 1990. Recombination of Salmonella Phage 1 flagellin genes generates new serovars. J. Bacteriol. 172: 2209-2216.
18. Smith, N.H., and Selander, R.K. 1990. Sequence invariance of the antigen-coding central region of the phase 1 flagellar filament gene (fliC) among strains of Salmonella typhimurium. J. Bacteriol. 172: 603-609.
19. Yamada, S., Matsushita, S., Kai, A., Sasaki. M., Tsuji, A., Kanemitsu, T., Yamashita, N., Anzai, E., and Kudoh, Y. 1993. Detection of verocytotoxin from stool and serological testing of patients with diarrhea caused by Escherichia coli O157:H7. Microbiol. Immunol. 37: 111-118.