Uncoupling of the ATPase activity from the branch migration activity of RuvAB protein complexes containing both wild-type and ATPase-defective RuvB proteins

Genes to Cells

Volumn 8, Issue 9, 2003, Pages 721-730

  Hishida, Takashi ^a   Iwasaki, Hiroshi ^b   Han, Yong Woon ^a   Ohnishi, Takayuki ^a   Shinagawa, Hideo ^a  

^a OSAKA UNIVERSITY   (Japan)

^b YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; DNA BINDING PROTEIN; DOUBLE STRANDED DNA; PROTEIN RUVA; PROTEIN RUVB; RECA PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DNA BINDING; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE MUTATION; HOLLIDAY JUNCTION; HOMOLOGOUS RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS; PROTEIN LOCALIZATION; PROTEIN TRANSPORT;

ADENOSINE TRIPHOSPHATASES; BACTERIAL PROTEINS; DNA HELICASES; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; MACROMOLECULAR SUBSTANCES; MUTATION; REC A RECOMBINASES; RECOMBINATION, GENETIC;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0141635255     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.2003.00670.x     Document Type: Article

References (31)

1. Cox, M.M., McEntee, K. & Lehman, I.R. (1981) A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli. J. Biol. Chem. 256, 4676-4678.
2. Dunderdale, H.J., Benson, F.E., Parsons, C.A., Sharples, G.J., Lloyd, R.G. & West, S.C. (1991) Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins. Nature 354, 506-510.
3. Han, Y.W., Iwasaki, H., Miyata, T., et al. (2001) A unique betahairpin protruding from AAA+ ATPase domain of RuvB motor protein is involved in the interaction with RuvA DNA recognition protein for branch migration of Holliday junctions. J. Biol. Chem. 276, 35024-35028.
4. Hishida, T., Iwasaki, H., Yagi, T. & Shinagawa, H. (1999) Role of Walker motif A of RuvB protein in promoting branch migration of Holliday junctions. Walker motif A mutations affect ATP binding, ATP hydrolyzing, and DNA binding activities of RuvB. J. Biol. Chem. 274, 25335-25342.
5. Holliday, R. (1964) A mechanism for gene conversion in fungi. Genet. Res. 5, 282-304.
6. + class helicase and motor protein for Holliday junction branch migration. Mol. Microbiol. 36, 528-538.
7. Iwasaki, H., Takahagi, M., Nakata, A. & Shinagawa, H. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 6, 2214-2220.
8. Iwasaki, H., Takahagi, M., Shiba, T., Nakata, A. & Shinagawa, H. (1991) Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure. EMBO J. 10, 4381-4389.
9. Iype, L.E., Wood, E.A., Inman, R.B. & Cox, M.M. (1994) RuvA and RuvB proteins facilitate the bypass of heterologous DNA insertions during RecA protein-mediated DNA strand exchange. J. Biol. Chem. 269, 24967-24978.
10. Kowalczykowski, S.C., Dixon, D.A., Eggleston, A.K., Lauder, S.D. & Rehrauer,W.M. (1994) Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58, 401-465.
11. Marrione, P.E. & Cox, M.M. (1995) RuvB protein-mediated ATP hydrolysis: functional asymmetry in the RuvB hexamer. Biochemistry 34, 9809-98018.
12. Marrione, P.E. & Cox, M.M. (1996) Allosteric effects of RuvA protein, ATP, and DNA on RuvB protein-mediated ATP hydrolysis. Biochemistry 35, 11228-11238.
13. Mézard, C., Davies, A.A., Stasiak, A. & West, S.C. (1997) Biochemical properties of RuvBD113N: a mutation in helicase motifII of the RuvB hexamer affects DNA binding and ATPase activities. J. Mol. Biol. 271, 704-717.
14. Mitchell, A.H. & West, S.C. (1994) Hexameric rings of Escherichia coli RuvB protein. Cooperative assembly, processivity and ATPase activity. J. Mol. Biol. 243, 208-215.
15. Müler, B., Tsaneva, I.R. & West, S.C. (1993) Branch migration of Holliday junctions promoted by the Escherichia coli RuvA and RuvB proteins. I. Comparison of RuvAB- and RuvB-mediated reactions. J. Biol. Chem. 268, 17179-17184.
16. +: a class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes. Genome Res. 9, 27-43.
17. Nishino, T., Ariyoshi, M., Iwasaki, H., Shinagawa, H. & Morikawa, K. (1998) Functional analyses of the domain structure in the Holliday junction binding protein RuvA. Structure 6, 11-21.
18. + superfamily ATPases: common structure - diverse function. Genes Cells 6, 575-597.
19. Parsons, C.A., Tsaneva, I., Lloyd, R.G. & West, S.C. (1992) Interaction of Escherichia coli RuvA and RuvB proteins with synthetic Holliday junctions. Proc. Natl. Acad. Sci. USA 89, 5452-5456.
20. Parsons, C.A. & West, S.C. (1993) Formation of a RuvAB-Holliday junction complex in vitro. J. Mol. Biol. 232, 397-405.
21. Patel, S.S., Hingorani, M.M. & Ng, W.M. (1994) The K318A mutant of bacteriophage T7 DNA primase-helicase protein is deficient in helicase but not primase activity and inhibits primase-helicase protein wild-type activities by heterooligomer formation. Biochemistry 33, 7857-7868.
22. Putnam, C.D., Clancy, S.B., Tsuruta, H., Gonzalez, S., Wetmur, J.G. & Tainer, J.A. (2001) Structure and mechanism of the RuvB Holliday junction branch migration motor. J. Mol. Biol. 311, 297-310.
23. Rafferty, J.B., Sedelnikova, S.E., Hargreaves, D., et al. (1996) Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction. Science. 274, 415-421.
24. Shiba, T., Iwasaki, H., Nakata, A. & Shinagawa, H. (1991) SOS-inducible DNA repair proteins, RuvA and RuvB, of Escherichia coli: Functional interactions between RuvA and RuvB for ATP hydrolysis and renaturation of the cruciform structure in supercoiled DNA. Proc. Natl. Acad. Sci. USA 88, 8445-8449.
25. Shiba, T., Iwasaki, H., Nakata, A. & Shinagawa, H. (1993) Escherichia coli RuvA and RuvB proteins involved in recombination repair: physical properties and interactions with DNA. Mol. Gen. Genet. 237, 395-399.
26. Shinagawa, H. & Iwasaki, H. (1996) Processing the Holliday junction in homologous recombination. Trends Biochem. Sci. 21, 107-111.
27. Tsaneva, I.R., Illing, G., Lloyd, R.G. & West, S.C. (1992b) Purification and properties of the RuvA and RuvB proteins of Escherichia coli. Mol. Gen. Genet. 235, 1-10.
28. Tsaneva, I.R., Müler, B. & West, S.C. (1992a) ATP-dependent branch migration of Holliday junctions promoted by the RuvA and RuvB proteins of E. coli. Cell 69, 1171-1180.
29. West, S.C. (1997) Processing of recombination intermediates by the RuvABC proteins. Annu. Rev. Genet. 31, 213-244.
30. Yamada, K., Kunishima, N., Mayanagi, K., et al. (2001) Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8. Proc, Natl. Acad. Sci. USA 98, 1442-1447.
31. Yamada, K., Miyata, T., Tsuchiya, D., et al. (2002) Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery. Mol. Cell 10, 671-681.