Differential response to low temperature of two Δ6 fatty acid desaturases from Mucor circinelloides

Applied Microbiology and Biotechnology

Volumn 62, Issue 4, 2003, Pages 362-368

  Michinaka, Y ^a   Aki, T ^a   Shimauchi, T ^b   Nakajima, T ^b   Kawamoto, S ^a   Shigeta, S ^a   Suzuki, O ^c   Ono, K ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b Idemitsu Technofine Co   (Japan)

^c Ikeura Patent Office   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA LINOLENIC ACID; ISOENZYME; LINOLEIC ACID; LINOLEOYL COENZYME A DESATURASE; MESSENGER RNA; RECOMBINANT PROTEIN;

FATTY ACID;

ARTICLE; COLD TREATMENT; ENZYME ACTIVITY; FATTY ACID ANALYSIS; GENE EXPRESSION; MOLECULAR CLONING; MORTIERELLA; MORTIERELLA ALPINA; MORTIERELLA ROUXII; MUCOR; MUCOR CIRCINELLOIDES; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; RNA TRANSCRIPTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; FATTY ACID DESATURASES; FATTY ACIDS; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; MOLECULAR SEQUENCE DATA; MUCOR; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; TEMPERATURE;

AMYLOMYCES ROUXII; MORTIERELLA; MORTIERELLA ALPINA; MUCOR; MUCOR CIRCINELLOIDES; MUCORALES; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0141483159     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1326-3     Document Type: Article

References (27)

1. Aguilar PS, Hernandez-Arriaga AM, Cybulski LE, Erazo AC, de Mendoza D (2001) Molecular basis of thermosensing: a two-component signal transduction thermometer in Bacillus subtilis. EMBO J 20:1681-1691
2. Aki T, Shimada Y, Inagaki K, Higashimoto H, Kawamoto S, Shigeta S, Ono K, Suzuki O (1999) Molecular cloning and functional characterization of rat Δ-6 fatty acid desaturase. Biochem Biophys Res Commun 255:575-579
3. Aki T, Nagahata Y, Ishihara K, Tanaka Y, Morinaga T, Higashiyama K, Akimoto K, Fujikawa S, Kawamoto S, Shigeta S, Ono K, Suzuki O (2001) Production of arachidonic acid by filamentous fungus, Mortierella alliacea strain YN-15. J Am Oil Chem Soc 78:599-604
4. Berberich T, Harada M, Sugawara K, Kodama H, Iba K, Kusano T (1998) Two maize genes encoding omega-3 fatty acid desaturase and their differential expression to temperature. Plant Mol Biol 36:297-306
5. Cho HP, Nakamura MT, Clarke SD (1999) Cloning, expression, and nutritional regulation of the mammalian Δ-6 desaturase. J Biol Chem 274:471-477
6. Cook HW (1996) Fatty acid desaturation and chain elongation in eukaryotes, In: Vance DE, Vance JE (eds) Biochemistry of lipids, lipoproteins and membranes. Elsevier, Amsterdam, pp 129-152
7. Fukuchi-Mizutani M, Tasaka Y, Tanaka Y, Ashikari T, Kusumi T, Murata N (1998) Characterization of Δ9 acyl-lipid desaturase homologues from Arabidopsis thaliana. Plant Cell Physiol 39:247-253
8. Gibson S, Arondel V, Iba K, Somerville C (1994) Cloning of a temperature-regulated gene encoding a chloroplast ω-3 desaturase from Arabidopsis thaliana. Plant Physiol 106:1615-1621
9. Horrobin DF (1992) Nutritional and medical importance of γ-linolenic acid. Prog Lipid Res 31:163-194
10. Inagaki K, Aki T, Fukuda Y, Kawamoto S, Shigeta S, Ono K, Suzuki O (2002) Identification and expression of a rat fatty acid elongase involved in the biosynthesis of C18 fatty acids. Biosci Biotechnol Biochem 66:613-621
11. Kaestner KH, Ntambi JM, Kelly TJ, Jr., Lane MD (1989) Differentiation-induced gene expression in 3T3-L1 preadipocytes. A second differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem 264:14755-14761
12. Laoteng K, Mannontarat R, Tanticharoen M, Cheevadhanarak S (2000) Δ6-desaturase of Mucor rouxii with high similarity to plant Δ6-desaturase and its heterologous expression in Saccharomyces cerevisiae. Biochem Biophys Res Commun 279:17-22
13. Luthria DL, Sprecher H (1993) 2-Alkenyl-4,4-dimethyloxazolines as derivatives for the structural elucidation of isomeric unsaturated fatty acids. Lipids 28:561-564
14. MacKenzie DA, Carter AT, Wongwathanarat P, Eagles J, Salt J, Archer DB (2002) A third fatty acid Δ9-desaturase from Mortierella alpina with a different substrate specificity to ole1p and ole2p. Microbiology 148:1725-1735
15. McConn M, Hugly S, Browse J, Somerville C (1994) A mutation at the fad8 locus of Arabidopsis identifies a second chloroplast omega-3 desaturase. Plant Physiol 106:1609-1614
16. Nakagawa Y, Sakumoto N, Kaneko Y, Harashima S (2002) Mga2p is a putative sensor for low temperature and oxygen to induce OLE1 transcription in Saccharomyces cerevisiae. Biochem Biophys Res Commun 291:707-713
17. Napier JA, Hey SJ, Lacey DJ, Shewry PR (1998) Identification of a Caenorhabditis elegans Δ6-fatty-acid-desaturase by heterologous expression in Saccharomyces cerevisiae. Biochem J 330:611-614
18. Panpoom S, Los DA, Murata N (1998) Biochemical characterization of a Δ12 acyl-lipid desaturase after overexpression of the enzyme in Escherichia coli. Biochim Biophys Acta 1390:323-332
19. Saito T, Morio T, Ochiai H (2000) A second functional Δ5 fatty acid desaturase in the cellular slime mould Dictyostelium discoideum. Eur J Biochem 267:1813-1818
20. Sakuradani E, Kobayashi M, Shimizu S (1999) Δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus. Gene cloning and its heterologous expression in a fungus, Aspergillus. Gene 238:445-453
21. Sayanova O, Smith MA, Lapinskas P, Stobart AK, Dobson G, Christie WW, Shewry PR, Napier JA (1997) Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of Δ6-desaturated fatty acids in transgenic tobacco. Proc Natl Acad Sci USA 94:4211-4216
22. Suzuki I, Los DA, Murata N (2000) Perception and transduction of low-temperature signals to induce desaturation of fatty acids. Biochem Soc Trans 28:628-630
23. Suzuki O (1989) Production of γ-linolenic acid by fungi and its industrialization, In: World Conference on Biotechnology for Fats and Oils Industry. AOCS, Hamburg, pp 110-116
24. Vigh L, Los DA, Horvath I, Murata N (1993) The primary signal in the biological perception of temperature: Pd-catalyzed hydrogenation of membrane lipids stimulated the expression of the desA gene in Synechocystis PCC6803. Proc Natl Acad Sci USA 90:9090-9094
25. Watts JL, Browse J (2000) A palmitoyl-CoA-specific Δ9 fatty acid desaturase from Caenorhabditis elegans. Biochem Biophys Res Commun 272:263-269
26. Wongwathanarat P, Michaelson LV, Carter AT, Lazarus CM, Griffiths G, Stobart AK, Archer DB, MacKenzie DA (1999) Two fatty acid Δ9-desaturase genes, ole1 and ole2, from Mortierella alpina complement the yeast ole1 mutation. Microbiology 145:2939-2946
27. Zheng Y, Prouty SM, Harmon A, Sundberg JP, Stenn KS, Parimoo S (2001) Scd3-a novel gene of the stearoyl-CoA desaturase family with restricted expression in skin. Genomics 71:182-191