A Polarized Microtubule Array for Kinesin-Powered Nanoscale Assembly and Force Generation

Nano Letters

Volumn 2, Issue 10, 2002, Pages 1131-1135

  Brown, Timothy B ^a   Hancock, William O ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0141470150     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl025636y     Document Type: Article

References (29)

1. Hirokawa, N. Science 1998, 279, 519-526.
2. Kull, F. J.; Sablin, E. P.; Lau, R.; Fletterick, R. J.; Vale, R. D. Nature 1996, 380, 550-555.
3. Yang, J. T.; Laymon, R. A.; Goldstein, L. S. Cell 1989, 56, 879-889.
4. Nogales, E.; Whittaker, M.; Milligan, R. A.; Downing, K. H. Cell 1999, 96, 79-88.
5. Limberis, L.; Magda, J. J.; Stewart, R. J. Nona Lett. 2001, 1, 277-280.
6. Dennis, J. R.; Howard, J.; Vogel, V. Nanotechnology 1999, 10, 232-236.
7. Hess, H.; Clemmens, J.; Qin, D.; Howard, J.; Vogel, V. Nano Lett. 2001, 1, 235-239.
8. Hiratsuka, Y.; Tada, T.; Oiwa, K.; Kanayama, T.; Uyeda, T. Q. Biophys. J. 2001, 81, 1555-1561.
9. Stracke, R.; Bohm, K. J.; Burgold, J.; Schacht, H.-J.; Unger, E. Nanotechnology 2000, 11, 52-56.
10. Limberis, L.; Stewart, R. J. Nanotechnology 2000, 11, 47-51.
11. Hunt, A. J.; Howard, J. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11653-11657.
12. Mitchison, T.; Kirschner, M. Nature 1984, 312, 237-242.
13. Vale, R. D.; Schnapp, B. J.; Mitchison, T.; Steuer, E.; Reese, T. S.; Sheetz, M. P. Cell 1985, 43, 623-632.
14. Williams, R. C., Jr.; Lee, J. C. Methods Enzymol. 1982, 85(Pt B), 376-385.
15. Hyman, A.; Drechsel, D.; Kellogg, D.; Salser, S.; Sawin, K.; Steffen, P.; Wordeman, L.; Mitchison, T. Methods Enzymol. 1991, 196, 478-485.
16. 2, pH 6.85) for 20 min at 37 °C. BRB80 buffer was used for all solutions unless otherwise noted.
17. Full-lenglh Drosophila conventional kinesin, purified as previously described (ref 26) was used at a concentration of 1-5 μg/mL. Before kinesin adsorption, surfaces were exposed to 0.5 mg/mL casein for 3 min to prevent motor denaturation. Any unbound motors were washed off with BRB80 buffer supplemented with 0.2 mg/mL casein and 0.1 mM MgATP.
18. Hancock, W. O.; Howard, J. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 13147-13152.
19. Farrell, C. M.; Mackey, A. T.; Klumpp, L. M.; Gilbert, S. P. J. Biol. Chem. 2002, 277, 17079-17087.
20. Song, H.; Endow, S. A. Nature 1998, 396, 587-590.
21. Wittmann, T.; Waterman-Storer, C. M. J. Cell Sci. 2001, 114, 3795-3803.
22. Yvon, A. M.; Wadsworth, P. J. Cell Biol. 2000, 151, 1003-1012.
23. Schulze, E.; Kirschner, M. Nature 1988, 334, 356-359.
24. Kirschner, M.; Schulze, E. J. Cell Sci. Suppl. 1986, 5, 293-310.
25. Schnitzer, M. J.; Visscher, K.; Block, S. M. Nature Cell Biol. 2000, 2, 718-723.
26. Hancock, W. O.; Howard, J. J. Cell Biol. 1998, 140, 1395-1405.
27. Galbraith, C. G.; Sheetz, M. P. J. Cell Biol. 1999, 147, 1313-1324.
28. Dembo, M.; Wang, Y. L. Biophys. J. 1999, 76, 2307-2316.
29. Berg, H. C. Random Walks in Biology, 2nd ed.; Princeton University Press: Princeton, NJ, 1993.