Elevated plasma homocysteine leads to alterations in fibrin clot structure and stability: Implications for the mechanism of thrombosis in hyperhomocysteinemia

Journal of Thrombosis and Haemostasis

Volumn 1, Issue 2, 2003, Pages 300-306

  Sauls, D L ^a,b   Wolberg, A S ^a,b,c   Hoffman, M ^a,b  

^a DURHAM VA MEDICAL CENTER   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Cardiovascular disease; Fibrinogen; Fibrinolysis; Homocysteine; Thrombosis

Indexed keywords

FIBRIN; FIBRINOGEN; HOMOCYSTEINE; PLASMIN;

ANIMAL; ARTICLE; BLOOD; BLOOD CLOTTING; HYPERHOMOCYSTEINEMIA; IN VITRO STUDY; ISOLATION AND PURIFICATION; METABOLISM; RABBIT; SCANNING ELECTRON MICROSCOPY; THROMBOSIS; ULTRASTRUCTURE;

ANIMALS; BLOOD COAGULATION; FIBRIN; FIBRINOGEN; HOMOCYSTEINE; HYPERHOMOCYSTEINEMIA; MICROSCOPY, ELECTRON, SCANNING; PLASMIN; RABBITS; THROMBOSIS;

EID: 0141450396     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1046/j.1538-7836.2003.00053.x     Document Type: Article

References (33)

1. Aronow WS, Ahn C, Gutstein H. Increased plasma homocysteine is an independent predictor of new atherothrombotic brain infarction in older persons. Am J Cardiol 2000; 86: 585-6, A510.
2. Eichinger S, Stumpflen A, Hirschl M, Bialonczyk C, Herkner K, Stain M, Schneider B, Pabinger I, Lechner K, Kyrle PA. Hyperhomocysteinemia is a risk factor of recurrent venous thromboembolism. Thromb Haemost 1998; 80: 566-9.
3. Lentz SR. Mechanisms of thrombosis in hyperhomocysteinemia. Curr Opin Hematol 1998; 5: 343-9.
4. Malinow MR. Homocyst(e)ine and arterial occlusive diseases. J Intern Med 1994; 236: 603-17.
5. Eberhardt RT, Forgione MA, Cap A, Leopold JA, Rudd MA, Trolliet M, Heydrick S, Stark R, Klings ES, Moldovan NI, Yaghoubi M, Goldschmidt-Clermont PJ, Farber HW, Cohen R, Loscalzo J. Endothelial dysfunction in a murine model of mild hyperhomocyst(e) inemia. J Clin Invest 2000; 106: 483-91.
6. Olszewski AJ, McCully KS. Homocysteine metabolism and the oxidative modification of proteins and lipids. Free Radic Biol Med 1993; 14: 683-93.
7. Blom HJ. Consequences of homocysteine export and oxidation in the vascular system. Semin Thromb Hemost 2000; 26: 227-32.
8. Sengupta S,Wehbe C, Majors AK, Ketterer ME, DiBello PM, Jacobsen DW. Relative roles of albumin and ceruloplasmin in the formation of homocystine, homocysteine-cysteine-mixed disulfide, and cystine in circulation. J Biol Chem 2001; 276: 46896-904.
9. Spindel E, McCully KS. Conversion of methionine to homocysteine thiolactone in liver. Biochim Biophys Acta 1974; 343: 687-91.
10. Jakubowski H. Metabolism of homocysteine thiolactone in human cell cultures. Possible mechanism for pathological consequences of elevated homocysteine levels. J Biol Chem 1997; 272: 1935-42.
11. Mudd SH, Matorin AI, Levy HL. Homocysteine thiolactone: failure to detect in human serum or plasma. Res Commun Chem Pathol Pharmacol 1989; 63: 297-300.
12. Kendall PA. Thiolation of proteins with homocysteine thiolactone: preparation of immunoglobulin G heavily labelled with methylmercury. Biochim Biophys Acta 1972; 257: 83-100.
13. Leanza WJ, Chupak LS, Tolman RL, Marburg S. Acidic derivatives of homocysteine thiolactone: utility as anionic linkers. Bioconjug Chem 1992; 3: 514-8.
14. Jakubowski H. Protein homocysteinylation: possible mechanism underlying pathological consequences of elevated homocysteine levels. FASEB J 1999; 13: 2277-83.
15. Lijnen HR, Collen D. Mechanisms of plasminogen activation by mammalian plasminogen activators. Enzyme 1988; 40: 90-6.
16. Higgins DL, Vehar GA. Interaction of one-chain and two-chain tissue plasminogen activator with intact and plasmin-degraded fibrin. Biochemistry 1987; 26: 7786-91.
17. Radcliffe R. A critical role of lysine residues in the stimulation of tissue plasminogen activator by denatured proteins and fibrin clots. Biochim Biophys Acta 1983; 743: 422-30.
18. Koopman J, Haverkate F, Grimbergen J, Lord ST, Mosesson MW, DiOrio JP, Siebenlist KS, Legrand C, Soria J, Soria C. Molecular basis for fibrinogen Dusart (A alpha 554 Arg→Cys) and its association with abnormal fibrin polymerization and thrombophilia. J Clin Invest 1993; 91: 1637-43.
19. Collet JP, Soria J, Mirshahi M, Hirsch M, Dagonnet FB, Caen J, Soria C. Dusart syndrome: a new concept of the relationship between fibrin clot architecture and fibrin clot degradability: hypofibrinolysis related to an abnormal clot structure. Blood 1993; 82: 2462-9.
20. McCully KS, Ragsdale BD. Production of arteriosclerosis by homocysteinemia. Am J Pathol 1970; 61: 1-11.
21. Furlan M. Purification of fibrinogen. Curr Probl Clin Biochem 1984; 14: 133-45.
22. Wolberg AS, Gabriel DA, Hoffman M. Analyzing fibrin clot structure using a microplate reader. Blood Coagul Fibrinolys 2002; 13: 533-9.
23. Carr ME, Gabriel DA. Dextran-induced changes in fibrin fiber size and density based on wavelength dependence of gel turbidity. Macromolecules 1980; 13: 1473-7.
24. Gruber A, Mori E, del Zoppo GJ, Waxman L, Griffin JH. Alteration of fibrin network by activated protein C. Blood 1994; 83: 2541-8.
25. Langer BG, Hong SK, Schmelzer CH, Bell WR. Deglycosylation of a native, protease-sensitive glycoprotein by peptide N-glycosidase F without protease inhibitors. Anal Biochem 1987; 166: 212-7.
26. Nickerson JM, Fuller GM. Modification of fibrinogen chains during synthesis. glycosylation of B beta and gamma chains. Biochemistry 1981; 20: 2818-21.
27. Gabriel DA, Muga K, Boothroyd EM. The effect of fibrin structure on fibrinolysis. J Biol Chem 1992; 267: 24259-63.
28. Durand P, Lussier-Cacan S, Blache D. Acute methionine load-induced hyperhomocysteinemia enhances platelet aggregation, thromboxane biosynthesis, and macrophage-derived tissue factor activity in rats. FASEB J 1997; 11: 1157-68.
29. Undas A, Williams EB, Butenas S, Orfeo T, Mann KG. Homocysteine inhibits inactivation of factor Va by activated protein C. J Biol Chem 2001; 276: 4389-97.
30. Lentz SR, Sobey CG, Piegors DJ, Bhopatkar MY, Faraci FM, Malinow MR, Heistad DD. Vascular dysfunction in monkeys with diet-induced hyperhomocyst(e)inemia. J Clin Invest 1996; 98: 24-9.
31. Watanabe M, Osada J, Aratani Y, Kluckman K, Reddick R, Malinow MR, Maeda N. Mice deficient in cystathionine beta-synthase: animal models for mild and severe homocyst(e)inemia. Proc Natl Acad Sci USA 1995; 92: 1585-9.
32. Haverkate F, Samama M. Familial dysfibrinogenemia and thrombophilia. Report on a study of the SSC Subcommittee on Fibrinogen. Thromb Haemost 1995; 73: 151-61.
33. Hajjar KA, Mauri L, Jacovina AT, Zhong F, Mirza UA, Padovan JC, Chait BT. Tissue plasminogen activator binding to the annexin II tail domain. Direct modulation by homocysteine. J Biol Chem 1998; 273: 9987-93.