Dimerization properties of a Xenopus laevis kinesin-II carboxy-terminal stalk fragment

EMBO Reports

Volumn 4, Issue 7, 2003, Pages 717-722

  De Marco, Valeria ^a   de Marco, Ario ^a   Goldie, Kenneth N ^a   Correia, John J ^b   Hoenger, Andreas ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF MISSISSIPPI MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; KINESIN; HOMODIMER; KINESIN 2;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; COMPLEX FORMATION; CONTROLLED STUDY; DIMERIZATION; ELECTRON MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; ULTRACENTRIFUGATION; XENOPUS LAEVIS; BACTERIAL STRAIN; COMPARATIVE STUDY; DENATURATION; MOLECULAR WEIGHT; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; SEDIMENTATION RATE;

AMINO ACID SEQUENCE; ANIMALS; CALCIUM-BINDING PROTEINS; CIRCULAR DICHROISM; DIMERIZATION; KINESIN; MOLECULAR SEQUENCE DATA; MUSCLE PROTEINS; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN FOLDING; ULTRACENTRIFUGATION; XENOPUS LAEVIS; XENOPUS PROTEINS;

STAPHYLOCOCCUS PHAGE 3A; XENOPUS LAEVIS;

EID: 0043066694     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.embor.embor884     Document Type: Article

References (19)

1. Chana, M., Tripet, B.P., Mant, C.T. & Hodges, R.S. (2002) The role of unstructured highly charged regions on the stability and specificity of dimerization of two-stranded α-helical coiled-coils: analysis of the neck-hinge region of he kinesin-like motor protein Kif3A. J. Struct. Biol., 137, 206-219.
2. Davis, G.D., Elisee, C., Newham, D.M. & Harrison, R.G. (1999) New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol. Bioeng., 65, 382-388.
3. De Marco, V., Burkhard, P., Le Bot, N., Vernos, I. & Hoenger, A. (2001) Analysis of heterodimer formation by Xklp3A/B, a newly cloned kinesin-II from Xenopus laevis. EMBO J., 20, 3370-3379.
4. Hirokawa, N. (1998) Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science, 279, 519-526.
5. Laue, T.M., Shah, B.D., Ridgeway, T.M. & Pelletier, S.L. (1992) in Analytical Ultracentrifugation in Biochemistry and Polymer Sciences (eds Harding, S.E., Rowe, A.J. & Horton, J.C.), 90-125. Royal Society of Chemistry, Cambridge, UK.
6. Le Bot, N., Antony, C., White, J., Karsenti, E. & Vernos, I. (1998) Role of Xklp3, a subunit of the Xenopus kinesin II heterotrimeric complex, in membrane transport between the endoplasmic reticulum and the Golgi apparatus. J. Cell Biol., 143, 1559-1573.
7. Liu, S. & Stafford, W.F. (1995) An optical thermometer for direct measurement of cell temperature in the Beckman instruments XL-A analytical ultracentrifuge. Anal. Biochem., 224, 199-202.
8. Marszalek, J.R. & Goldstein, L.S. (2000) Understanding the functions of kinesin-II. Biochim. Biophys. Acta, 1496, 142-150.
9. Muresan, V., Abramson, T., Lyass, A., Winter, D., Porro, E., Hong, F., Chamberlin, N.L. & Schnapp, B.J. (1998) KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles. Mol. Biol. Cell, 9, 637-652.
10. Philo, J.S. (2000) A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Anal. Biochem., 179, 151-163.
11. Rashid, D.J., Wedaman, K.P. & Scholey, J.M. (1995) Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP85/95. J. Mol. Biol., 252, 157-162.
12. Schuck, P., Perugini, M.A., Gonzales, N.R., Howlett, G.J. & Schubert. D. (2002) Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems. Biophys. J., 82, 1096-1111.
13. Sönnichsen, F.D., Van Eyk, J.E., Hodges, R.S. & Sykes, B.D. (1992) Effect of trifluoroethanol on protein secondary structure: an NMR and CD study using a synthetic actin peptide. Biochemistry, 31, 8790-8798.
14. Tan, S. (2001) A modular polycystronic expression system for overexpressing protein complexes in Escherichia coli. Protein Expr. Purif., 21, 224-234.
15. Tomoyasu, T., Mogk, A., Langen, H., Goloubinoff, P. & Bukau, B. (2001) Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol. Mol. Microbiol., 40, 397-413.
16. Tripet, B. & Hodges, R.S. (2002) Helix capping interactions stabilize the N-terminus of the kinesin neck coiled-coil. J. Struct. Biol., 137, 220-235.
17. Tuma, M.C., Zill, A., Le Bot, N., Vernos, I. & Gelfand, V. (1998) Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores. J. Cell Biol., 143, 1547-1558.
18. Vale, R.D. (2003) The molecular motor toolbox for intracellular transport. Cell, 112, 467-480.
19. Yang, Z. & Goldstein, L.S. (1998) Characterization of the KIF3C neural kinesin-like motor from mouse. Mol. Biol. Cell, 9, 249-261.