Biofunctionalization of Silica-Coated CdTe and Gold Nanocrystals

Nano Letters

Volumn 2, Issue 12, 2002, Pages 1363-1367

  Schroedter, Andrea ^a   Weller, Horst ^a   Eritja, Ramon ^b   Ford, William E ^c   Wessels, Jurina M ^c  

^a UNIVERSITY OF HAMBURG   (Germany)

^b INSTITUT DE BIOLOGIA MOLECULAR DE BARCELONA   (Spain)

^c Sony Deutschland GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0042975917     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl025779k     Document Type: Article

References (28)

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23. 2Te gas through 100 mL of cooled 50 mM NaOH.
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27. Synthesis of the oligonucleotide 100 up-thiol: 5′-thiolhexyl-phosphate-CTA CGT CGC TGA CTA CCT GCG TAG GTC CCT AGA TGG CTA ACT CGG TGC ATC GCT CAC TGG ATA CAT CAG TCC ATG AAT GAC TCG ATG ACT CAA TGA CTC G-3′ was synthesized using an Applied Biosystems 392 DNA synthesizer. The oligonucleotide was prepared on a 200-nmol scale using polystyrene supports (LV200, Applied Biosystems) and standard phosphoramidites (Applied Biosystems). The phosphoramidite of 6-hydroxyhexyl disulfide, protected with a dimethoxytrityl group (Glen Research), was used to introduce the thiol group at the terminal 5′ position. The thiol-modified oligonucleotide was deprotected with concentrated ammonia (0.05 M DTT, 16 h, 55°C) and desalted via an NAP-10 column prior to conjugation with gold nanoparticles.
28. 2 for 1 min and then rinsed with 10 drops of water while rotating at 5000 rpm on a spin coater. The solution of the conjugate was then applied to the substrate. After 2 min, the excess solution was removed by spinning at 5000 rpm while rinsing with 2 drops of water. Tapping-mode AFM measurements were made in air using either a MultiMode scanning probe microscope with a NanoScope IIIa controller and a NanoScope extender or a Dimension 3100 SPM system with a NanoScope IV controller (Digital Instruments, Santa Barbara, CA). Tetrahedral-tip silicon cantilevers (Olympus Optical OMCL-AC160TS series, resonance frequency 250-300 kHz) were used for all AFM measurements.