Intraspecies polymorphism of Cryptosporidium parvum revealed by PCR-restriction fragment length polymorphism (RFLP) and RFLP-single-strand conformational polymorphism analyses

Applied and Environmental Microbiology

Volumn 69, Issue 8, 2003, Pages 4720-4726

  Wu, Zhiliang ^a   Nagano, Isao ^a   Boonmars, Thidarut ^a   Nakada, Takumi ^a   Takahashi, Yuzo ^a  

^a GIFU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; PROTEINS;

GLYCOPROTEINS;

MICROBIOLOGY;

GLYCOPROTEIN;

POLYMORPHISM;

AMINO ACID SEQUENCE; ARTICLE; CATTLE; CPGP15 GENE; CPGP40 GENE; CRYPTOSPORIDIUM PARVUM; DNA POLYMORPHISM; DNA SEQUENCE; GENETIC ANALYSIS; GENOTYPE; GEOGRAPHIC ORIGIN; GOAT; HOST; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTOZOAL GENETICS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM; SPECIES DIVERSITY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CRYPTOSPORIDIUM PARVUM; DNA, PROTOZOAN; GENOTYPE; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PROTOZOAN PROTEINS;

BOS TAURUS; CAPRA HIRCUS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 0042530233     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.8.4720-4726.2003     Document Type: Article

References (20)

1. Gobet, P., and S. Toze. 2001. Sensitive genotyping of Cryptosporidium parvum by PCR-RFLP analysis of the 70-kilodalton heat shock protein (HSP70) gene. FEMS Microbiol. Lett. 200:37-41.
2. Leav, B. A., M. R. Mackay, A. Anyanwu, R. M. O'Connor, A. M. Cevallos, G. Kindra, N. C. Rollins, M. L. Bennish, R. G. Nelson, and H. D. Ward. 2002. Analysis of sequence diversity at the highly polymorphic Cpgp40/15 locus among Cryptosporidium isolates from human immunodeficiency virus-infected children in South Africa. Infect. Immun. 70:3881-3890.
3. Morgan, U. M., C. C. Constantine, D. A. Forbes, and R. C. A. Thompson. 1997. Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis. J. Parasitol. 83:825-830.
4. Morgan, U. M., D. A. Forbes, and R. C. Thompson. 1998. Molecular epidemiology of Cryptosporidium parvum. Eur. J. Protistol. 34:262-266.
5. Peng, M. M., L. Xiao, A. R. Freeman, M. J. Arrowood, A. A. Escalante, A. C. Weltman, C. S. Ong, W. R. Mac Kenzie, A. A. Lal, and C. B. Beard. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerg. Infect. Dis. 3:567-573.
6. Rayer, R., U. M. Morgan, and S. L. Upton. 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol. 30:1305-1322.
7. Spano, F., L. Putignani, A. Crisanti, P. Sallicandro, U. M. Morgan, S. M. Le Blancq, L. Tchack, S. Tzipori, and G. Widmer. 1998. A multilocus genotypic analysis of Cryptosporidium parvum from different hosts and geographical origins. J. Clin. Microbiol. 36:3255-3259.
8. Spano, F., L. Putignani, S. Guida, and A. Crisanti. 1999. Cryptosporidium parvum: PCR-RFLP analysis of the TRAP-C1 (thrombosondin-related adhesive protein of Cryptosporidium-1) gene discriminates between two alleles differentially associated with parasite isolates of animal and human origin. Exp. Parasitol. 90:195-198.
9. Spano, F., L. Putignani, J. McLauchlin, D. P. Casemore, and A. Crisanti. 1997. PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiol. Lett. 150:209-217.
10. Spano, F., L. Putignani, S. Naitza, C. Puri, S. Wright, and A. Crisanti. 1998. Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family. Mol. Biochem. Parasitol. 92:147-162.
11. Strong, W. B., J. Gut, and R. G. Nelson. 2000. Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products. Infect. Immun. 68:4117-4134.
12. Sulaiman, I. M., U. M. Morgan, R. C. A. Thompson, A. A. Lal, and L. Xiao. 2000. Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP) gene. Appl. Environ. Microbiol. 66:2385-2391.
13. Sulaiman, I. M., L. Xiao, C. Yang, L. Escalante, A. Moore, C. B. Beard, M. J. Arrowood, and A. A. Lal. 1998. Differentiating human from animal isolates of Cryptosporidium parvum. Emerg. Infect. Dis. 4:681-685.
14. Wu, Z., I. Nagano, A. Matsuo, E. Pozio, and Y. Takahashi. 1999. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the identification of Trichinella isolates. Parasitology 118:211-218.
15. Wu, Z., I. Nagano, A. Matsuo, S. Uga, I. Kimata, M. Iseki, and Y. Takahashi. 2000. Specific PCR primers for Cryptosporidium parvum with extra high sensitivity. Mol. Cell. Probes 14:33-39.
16. Wu, Z., I. Nagano, T. Nakada, and Y. Takahashi. 1999. DNA fingerprints of Trichinella as revealed by restriction fragment length polymorphism and single-strand conformational polymorphism (RFLP-SSCP). Mol. Cell. Probes 14:291-297.
17. Xiao, L., L. Escalante, C. Yang, I. Sulaiman, A. A. Escalante, R. J. Montali, R. Fayer, and A. A. Lal. 1999. Phylogenetic analysis of Cryptosporidium parasites based on the small subunit ribosomal RNA gene locus. Appl. Environ. Microbiol. 65:1578-1583.
18. Xiao, L., J. Kimor, U. M. Morgan, I. M. Sulaiman, R. C. A. Thompson, and A. A. Lal. 2000. Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites. Appl. Environ. Microbiol. 66:5499-5502.
19. Xiao, L., U. M. Morgan, R. Fayer, R. C. A. Thompsom, and A. A. Lal. 2000. Cryptosporidium systematics and implications for public health. Parasitol. Today 16:287-292.
20. Xiao, L., U. M. Morgan, J. Limor, A. Escalante, M. Arrowood, W. Shulaw, R. C. A. Thompson, R. Fayer, and A. A. Lal. 1999. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl. Environ. Microbiol. 65:3386-3391.