Sensitive genotyping of Cryptosporidium parvum by PCR-RFLP analysis of the 70-kilodalton heat shock protein (HSP70) gene

FEMS Microbiology Letters

Volumn 200, Issue 1, 2001, Pages 37-41

  Gobet, Philippe ^a   Toze, Simon ^a  

^a CSIRO   (Australia)

Author keywords

70 kDa heat shock protein gene; Cryptosporidium parvum; Genotyping; Polymerase chain reaction; Restriction fragment length polymorphism

Indexed keywords

ENDONUCLEASE; HEAT SHOCK PROTEIN 70;

ARTICLE; CRYPTOSPORIDIUM PARVUM; DNA SEQUENCE; GENOTYPE; NONHUMAN; OOCYTE; PARASITE ISOLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPECIES DIFFERENCE;

ANIMALS; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA PRIMERS; GENES, PROTOZOAN; GENOTYPE; HSP70 HEAT-SHOCK PROTEINS; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RESTRICTION MAPPING;

CANIS FAMILIARIS; CRYPTOSPORIDIUM MELEAGRIDIS; CRYPTOSPORIDIUM PARVUM;

EID: 0035849679     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(01)00196-3     Document Type: Article

References (17)

1. The infectivity of Cryptosporidium parvum in healthy volunteers
2. Cryptosporidiosis in patients with AIDS
3. Epidemiology of Cryptosporidium: Transmission, detection and identification
4. Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence
5. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species
6. Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene
7. Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites
8. Evaluation of Cryptosporidium parvum genotyping techniques
9. Relevance of Cryptosporidium parvum hsp70 mRNA amplification as a tool to discriminate between viable and dead oocysts
10. Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR
11. New Cryptosporidium genotypes in HIV-infected persons
12. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru
13. An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum
14. Amplification product inactivation methods
15. Detection of Cryptosporidium parvum in formed human feces by a sensitive PCR-based assay including uracil-N-glycosylase inactivation
16. Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR