Disruption of Botrytis cinerea class I chitin synthase gene Bcchs1 results in cell wall weakening and reduced virulence

Fungal Genetics and Biology

Volumn 40, Issue 1, 2003, Pages 38-46

  Soulie M C ^a   Piffeteau, A ^b   Choquer, M ^a,b   Boccara, M ^a   Vidal Cros, A ^b  

^a INRA   (France)

^b CNRS   (France)

Author keywords

Botrytis cinerea; Cell wall mutant; Chitin; Chitin synthase; Phytopathogenic fungus

Indexed keywords

CHITIN SYNTHASE; GLUCAN GLUCOSIDASE;

ARTICLE; BCCHS1 GENE; BOTRYTIS CINEREA; CELL WALL; CONTROLLED STUDY; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; FUNGAL CELL; FUNGAL GENE; FUNGUS GROWTH; GENE AMPLIFICATION; GENE DISRUPTION; GENOME; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRULENCE;

BOTRYOTINIA FUCKELIANA; BOTRYTIS; BOTRYTIS CINEREA; FUNGI;

EID: 0042509904     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1087-1845(03)00065-3     Document Type: Article

References (39)

1. Adams D.J. Drug and pesticide resistance in fungi. Wolf C.R., Hayes J.D. Molecular Genetics of Drug Resistance. 1994;Harwood Academic Publishers, London.
2. Beth Din A., Specht C.A., Robbins P.W., Yarden O. Chs-4, a class IV chitin synthase gene from Neurospora crassa. Mol. Gen. Genet. 250:1996;214-222.
3. Bird D., Bradshaw R. Gene targeting is locus dependent in the filamentous fungus Aspergillus nidulans. Mol. Gen. Genet. 255:1997;219-225.
4. Bowen A.R., Chen-Wu J.L., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Classification of fungal chitin synthases. Proc. Natl. Acad. Sci. USA. 89:1992;519-523.
5. Bulawa C.E., Slater M., Cabib E., Au-Young J., Sburlati A., Lee Adair W., Robbins P.W. The Saccharomyces cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell. 46:1986;213-225.
6. Bulawa C.E. Csd2, csd3 and csd4 genes required for chitin synthesis in Saccharomyces cerevisiae: the csd2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis. Mol. Cell. Biol. 12:1992;1764-1776.
7. Bulawa C.E. Genetics and molecular biology of chitin synthesis in fungi. Annu. Rev. Microbiol. 47:1993;505-534.
8. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
9. Cabib E., Sburlati A., Bowers B., Silverman S.J. Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae. J. Cell. Biol. 108:1989;1665-1672.
10. Causier B.E., Milling R.J., Foster S.G., Adams D.J. Characterization of chitin synthase from Botrytis cinerea. Microbiology. 140:1994;2199-2205.
11. Chigira Y., Abe K., Gomi K., Nakajima T. ChsZ, a gene for a novel class of chitin synthase from a Aspergillus orizae. Curr. Genet. 41:2002;261-267.
12. Coley-Smith J.R., Verhoeff K., Jarvis W.R. The Biology of Botrytis. 1980;Academic Press, London.
13. Culp D.W., Dodge C.L., Miao Y., Li L., Sag-Ozkal D., Borgia P.T. The chsA gene from Aspergillus nidulans is necessary for maximal conidiation. FEMS Microbiol. Lett. 182:2000;349-353.
14. DiDomenico B. Novel antifungal drugs. Curr. Opin. Microbiol. 2:1999;509-515.
15. Elson L.A., Morgan W.T.J. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem. J. 27:1933;1824-1828.
16. Fontaine T., Simenel C., Dubreucq G., Adam O., Delepierre M., Lemoine J., Vorgias C.E., Diaquin M., Latge J.P. Molecular organization of the alkali-insoluble fraction of Aspergillus fumigatus cell wall. J. Biol. Chem. 275:2000;27594-27607.
17. Fritig B., Heitz T., Legrand M. Antimicrobial proteins in induced plant defense. Curr. Opin. Immunol. 10:1998;16-22.
18. Hamada W., Reignault Ph., Bompeix G., Boccara M. Transformation of Botrytis cinerea with the hygromycin B resistance gene hph. Curr. Genet. 26:1994;251-255.
19. Hintz W.E. Sequence analysis of the chitin synthase A gene of the Dutch elm pathogen Ophiostoma novo-ulmi indicates a close association with the human pathogen Sporothrix schenckii. Gene. 237:1999;215-221.
20. Kollar R., Petrakova E., Ashwell G., Robbins P.W., Cabib E. Architecture of the yeast cell wall. The linkage between chitin and. β (1,3) glucan J. Biol. Chem. 270:1995;1170-1178.
21. Kollar R., Reinhold B.B., Petrakova E., Yeh H.J.C., Ashwell G., Drgonova J., Kapteyn J.C., Klis F.M., Cabib E. Architecture of the yeast cell wall. β (1-6) glucan interconnects mannoprotein, β (1-3)-glucan and chitin J. Biol. Chem. 272:1997;17762-17775.
22. Lipke P.N., Ovalle R. Cell wall architecture in yeast: new structure and new challenges. J. Bacteriol. 180:1998;3735-3740.
23. Mellado E., Aufavre-Brown A., Gow N.A.R., Holden D.W. The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions. Mol. Microbiol. 20:1996;667-679.
24. Motoyama T., Fujiwara M., Kojima N., Horiuchi H., Ohta A., Takagi M. The Aspergillus nidulans genes chsA and chsD encode chitin synthases which have redundant functions in conidia formation. Mol. Gen. Genet. 253:1997;520-528.
25. Motoyama T., Kojima N., Horiuchi H., Ohta A., Takagi M. Isolation of a chitin synthase gene (chsC) of Aspergillus nidulans. Biosci. Biotech. Biochem. 58:1994;2254-2257.
26. Orlean P. Two chitin synthases in Saccharomyces cerevisiae. J. Biol. Chem. 262:1987;5732-5739.
27. Punt P.J., Oliver R.P., Dingemanse M.A., Pouwels P.H., Van den Hondel C.A.M.J.J. Transformation of Aspergillus nidulans based on the hygromycin B resistance marker from Escherichia coli. Gene. 56:1987;117-124.
28. Reignault P., Mercier M., Bompeix G., Boccara M. Pectin methyl esterase from Botrytis cinerea: physiological, biochemical and immunochemical studies. Microbiology. 140:1994;3249-3255.
29. Ruiz-Herrera J., Gonzalez-Prieto J.M., Ruiz-Medrano R. Evolution and phylogenetic relationships of chitin synthases from yeasts and fungi. FEMS Yeast Res. 1:2001;247-256.
30. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning. 1989;Cold Spring Harbor Laboratory Press, Plainview, NY.
31. Shaw J.A., Mol P.G., Bowers B., Silverman S.J., Valdivieso M.H., Duran A., Cabib E. The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J. Cell Biol. 114:1991;111-123.
32. Silverman S.J., Sburlati A., Slater M.L., Cabib E. Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 85:1988;4735-4739.
33. Specht C.A., Liu Y., Robbins P.W., Bulawa C.E., Iartchouk N., Winter K.R., Riggle P.J., Rhodes J.C., Dodge C.L., Culp D.W., Borgia P.T. The chsD and ChsE genes of Aspergillus nidulans and their roles in chitin synthesis. Fungal Genet. Biol. 20:1996;153-167.
34. Stintzi A., Heitz T., Prasad V., Wiedemann-Merdinoglu S., Kauffmann S., Geoffroy P., Legrand M., Fritig M. Plant "Pathogenesis-Related" proteins and their role in defense against pathogens. Biochimie. 75:1993;687-706.
35. Sudoh M., Tatsuno K., Ono N., Ohta A., Chibana H., Yamada-Okabe H., Arisawa M. The Candida albicans CHS4 gene complements a Saccharomyces cerevisiae skt5/chs4 mutation and is involved in chitin biosynthesis. Microbiology. 145:1999;1613-1622.
36. Wang Z., Zheng L., Liu H., Wang Q., Hauser M., Kauffman S., Becker J., Szaniszlo P. WdChs2p, a class I chitin synthase, together with WdChs3p (class III) contributes to virulence in Wangiella (Exophiala) dermatitidis. Infect. Immun. 69:2001;7517-7526.
37. Xoconostle-Cazares B., Specht C.A., Robbins P.W., Liu Y., Leon C., Ruiz-Herrera J. Umchs5, a gene coding for a class IV chitin synthase in Ustilago maydis. Fungal Genet. Biol. 22:1997;199-208.
38. Yabe T., Yamada-Okabe T., Kashara S., Furuichi Y., Nakajima T., Ichisima E., Arisawa M., Yamada-Okabe H. HKR1 encodes a cell surface protein that regulates both cell wall β-glucan synthesis and budding pattern in the yeast. Saccharomyces Cerevisiae. J. Bact. 178:1996;477-483.
39. Yanai K., Kojima N., Takaya N., Horiuchi H., Ohta A., Takagi M. Isolation and characterization of two chitin synthase genes from Aspergillus nidulans. Biosci. Biotech. Biochem. 58:1994;1828-1835.