Induction of adiponectin gene expression in human myotubes by an adiponectin-containing HEK293 cell culture supernatant

Diabetologia

Volumn 46, Issue 7, 2003, Pages 956-960

  Staiger, H ^a   Kausch, C ^a   Guirguis, A ^a   Weisser, M ^a   Maerker, E ^a   Stumvoll, M ^a   Lammers, R ^a   Machicao, F ^a   Haring H U ^a,b  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b Medical Clinic   (Germany)

Author keywords

Adipocytokines; Adiponectin; Insulin sensitivity; Skeletal muscle cells

Indexed keywords

ADIPONECTIN; INSULIN; MESSENGER RNA;

ARTICLE; CELL DIFFERENTIATION; CELL STRAIN; CELL STRAIN HEK293; CELL STRAIN HEPG2; CONTROLLED STUDY; DOWN REGULATION; EMBRYO; GENE CONTROL; GENE EXPRESSION; GENE INDUCTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INSULIN SENSITIVITY; MUSCLE CELL; MUSCLE FIBRIL; OBESITY; PRIORITY JOURNAL; RADIOIMMUNOASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKELETAL MUSCLE; SKIN FIBROBLAST; SUPERNATANT; WESTERN BLOTTING;

EID: 0042071576     PISSN: 0012186X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00125-003-1146-0     Document Type: Article

References (16)

1. Yamauchi T, Kamon J, Waki H et al. (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 7:941-946
2. Berg AH, Combs TP, Scherer PE (2002) Acrp30/adiponectin: an adipokine regulating glucose and lipid metabolism. Trends Endocrinol Metab 13:84-89
3. Berg AH, Combs TP, Du X et al. (2001) The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med 7:947-952
4. Combs TP, Berg AH, Obici S et al. (2001) Endogenous glucose production is inhibited by the adipose-derived protein acrp30. J Clin Invest 108:1875-1881
5. Fruebis J, Tsao TS, Javorschi S et al. (2001) Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidaton in muscle and causes weight loss in mice. Proc Natl Acad Sci USA 98:2005-2010
6. Maeda N, Shimomura I, Kishida K et al. (2002) Diet-induced insulin resistance in mice lacking adiponectin/ Acrp30. Nat Med 8:731-737
7. Fasshauer M, Klein J, Neumann S et al. (2002) Hormonal regulation of adiponectin gene expression in 3T3-L1 adipocytes. Biochem Biophys Res Commun 290:1084-1089
8. Kappes A, Löffler G (2000) Influences of ionomycin, dibutyryl-cycloAMP and tumour necrosis factor-alpha on intracellular amount and secretion of apM1 in differentiating primary human preadipocytes. Horm Metab Res 32:548-554
9. Halleux CM, Takahashi M, Delporte ML et al. (2001) Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue. Biochem Biophys Res Commun 288:1102-1107
10. Fasshauer M, Klein J, Neumann S et al. (2001) Adiponectin gene expression is inhibited by β-adrenergic stimulation via protein kinase A in 3T3-L1 adipocytes. FEBS Lett 507:142-146
11. Delporte ML, Funahashi T, Takahashi M et al. (2002) Pre- and post-translational negative effect of β-adrenoceptor agonists on adiponectin secretion: in vitro and in vivo studies. Biochem J: electronic publication ahead of print
12. Maeda N, Takahashi M, Funahashi T et al. (2001) PPARγ ligands increase expression and plasma concentrations of adiponectin, an adipose-derived protein. Diabetes 50:2094-2099
13. Combs TP, Wagner JA, Berger J et al. (2002) Induction of adipocyte complement-related protein of 30 kilodaltons by PPARγ agonists: a potential mechanism of insulin sensitization. Endocrinology 143:998-1007
14. Krützfeldt J, Kausch C, Volk A et al. (2000) Insulin signaling and action in cultured skeletal muscle cells from lean healthy humans with high and low insulin sensitivity. Diabetes 49:992-998
15. Sly WS, Grubb J (1979) Protocol for skin biopsy and fibroblast isolation. Methods Enzymol 58:444-450
16. Staiger H, Tschritter O, Kausch C et al. (2002) Human serum adiponectin levels are not under short-term negative control by free fatty acids in vivo. Horm Metab Res 34:601-603