Exposure to chronic, low-dose rate γ-radiation at Chornobyl does not induce point mutations in Big Blue® mice

Environmental and Molecular Mutagenesis

Volumn 42, Issue 1, 2003, Pages 11-18

  Wickliffe, Jeffrey K ^a,d   Bickham, Amy M ^a   Rodgers, Brenda E ^a   Chesser, Ronald K ^a,b   Phillips, Carleton J ^a   Gaschak, Sergey P ^b   Goryanaya, J A ^b   Chizhevsky, I ^c   Baker, Robert J ^a  

^a TEXAS TECH UNIVERSITY   (United States)

^b International Radioecology Laboratory   (Ukraine)

^c International Chornobyl Center   (Ukraine)

^d UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

Big Blue ; Chornobyl Chernobyl; Environmental radiation; Somatic mutations; Transgenic mice

Indexed keywords

GENOMIC DNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CHERNOBYL ACCIDENT; CONTROLLED STUDY; CORRELATION COEFFICIENT; CYTOGENETICS; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; FEMALE; GAMMA IRRADIATION; GAMMA RADIATION; LONG TERM EXPOSURE; LOW ENERGY RADIATION; MALE; MOUSE STRAIN; MUTATION RATE; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; POINT MUTATION; RADIATION EXPOSURE; RADIATION RESPONSE; RADIOSENSITIVITY; RISK ASSESSMENT; TRANSGENE;

ACCIDENTS, RADIATION; ANIMALS; DNA; DNA DAMAGE; DNA MUTATIONAL ANALYSIS; DOSE-RESPONSE RELATIONSHIP, RADIATION; FEMALE; GAMMA RAYS; MALE; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; MUTAGENESIS; POINT MUTATION; POWER PLANTS; RADIATION DOSAGE; RADIATION INJURIES; RADIOACTIVE POLLUTANTS; UKRAINE;

MUS MUSCULUS;

EID: 0042071492     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/em.10170     Document Type: Article

References (48)

1. Auerbach C. 1976. Mutation research: problems, results, and perspectives. New York: Chapman & Hall.
2. Baker RJ, Van Den Bussche RA, Wright AJ, Wiggins LE, Hamilton MJ, Reat EP, Smith MH, Lomakin MD, Chesser RK. 1996. High levels of genetic change in rodents of Chernobyl. Nature 380:707-708.
3. Baker RJ, Van Den Bussche RA, Wright AJ, Wiggins LE, Hamilton MJ, Reat EP, Smith MH, Lomakin MD, Chesser RK. 1997. Retraction: high levels of genetic change in rodents of Chernobyl. Nature 390:100.
4. Baker RJ, Makova KD, Chesser RK. 1999a. Microsatellites indicate a high frequency of multiple paternity in Apodemus (Rodentia). Mol Ecol 8:107-111.
5. Baker RJ, DeWoody JA, Wright AJ, Chesser RK. 1999b. On the utility of heteroplasmy in genotoxicity studies: an example from Chornobyl. Ecotoxicology 8:301-309.
6. Baker RJ, Bickham AM, Bondarkov M, Gaschak SP, Matson CW, Rodgers BE, Wickliffe JK, Chesser RK. 2001. Consequences of polluted environments on population structure: the bank vole (Clethrionomys glareolus) at Chornobyl. Ecotoxicology 10:211-216.
7. Buettner VL, Hill KA, Scaringe WA, Sommer SS. 2000. Evidence that proximal multiple mutations in Big Blue® transgenic mice are dependent events. Mutat Res 452:219-229.
8. 90strontium in small mammals at Chornobyl, Ukraine. Environ Toxicol Chem 19:305-312.
9. 90strontium from abiotic and biotic sources in rodents at Chornobyl, Ukraine. Environ Toxicol Chem 20:1927-1935.
10. de Boer JG. 1995. Software package for the management of sequencing projects using lacI transgenic animals. Environ Mol Mutagen 25:256-262.
11. de Boer JG, Erfle H, Walsh D, Holcroft J, Provost JS, Rogers B, Tindall KR, Glickman BW. 1997. Spectrum of spontaneous mutations in liver tissue of lacI transgenic mice. Environ Mol Mutagen 30:273-286.
12. De Souza-Pinto NC, Hogue BA, Bohr VA. 2001. DNA repair and aging in mouse liver: 8-oxodG glycosylase activity increases in mitochondrial but not in nuclear extracts. Free Radical Biol Med 30:916-923.
13. DeWoody JA. 1999. Nucleotide variation in the p53 tumor-suppressor gene of voles from Chernobyl, Ukraine. Mutat Res 439:25-36.
14. Doull J, Rozman KK. 2000. Using Haber's Law to define the margin of exposure. Toxicology 149:1-2
15. Dubrova YE, Nesterov VN, Krouchinsky NG, Ostapenko VA, Neumann R, Neil DL, Jeffreys AJ. 1996. Human minisatellite mutation rate after the Chernobyl accident. Nature 380:683-686.
16. Dubrova YE, Nesterov VN, Krouchinsky NG, Ostapenko VA, Vergnaud G, Giraudeau F, Buard J, Jeffreys AJ. 1997. Further evidence for elevated human minisatellite mutation rate in Belarus eight years after the Chernobyl accident. Mutat Res 381:267-278.
17. Eaton DL, Klaasen CD. 1996. Principles of toxicology. In: Klaasen CD, editor. Casarett and Doull's toxicology: the basic science of poisons. New York: McGraw-Hill. p 13-33.
18. Ellegren H, Lindgren G, Primmer CR, Moller AP. 1997. Fitness loss and germline mutations in barn swallows breeding in Chernobyl. Nature 389:593-596.
19. Farabaugh PJ. 1978. Sequence of the lacI gene. Nature 274:765-769.
20. Faul F, Erdfelder E. 1992. GPOWER: a priori, post-hoc, and compromise power analyses for MS-DOS (computer program). Bonn: Bonn University, Department of Psychology.
21. Grollman AP, Moriya M. 1993. Mutagenesis by 8-oxoguanine: an enemy within. Trends Genet 9:246-249.
22. Hoyes KP, Wadeson PJ, Sharma HL, Hendry JH, Morris ID. 1998. Mutation studies in lacI transgenic mice after exposure to radiation or cyclophosphamide. Mutagen 13:607-612.
23. Huo L, Nagasawa H, Little JB. 2001. HPRT mutants induced in bystander cells by very low fluences of alpha particles result primarily from point mutations. Radiat Res 156:521-525.
24. Jaworoski Z. 1997. Beneficial effects of radiation and regulatory policy. Australas Phys Eng Sci Med 20:125-138.
25. Jaworoski Z. 1999. Radiation dose and infinity: why collective dose is an absurd concept. Nuclear Report, Report on the 1999 UNSCEAR meeting, p 22-23,25.
26. Kirsch-Volders M, Aardema M, Elhajouji A. 2000. Concepts of threshold in mutagenesis and carcinogenesis. Mutat Res 464:3-11.
27. Kohler SW, Provost GS, Fieck A, Kretz PL, Bullock WO, Putman DL, Sorge JA, Short JM. 1991. Analysis of spontaneous and induced mutations in transgenic mice using a lambda ZAP/lacI shuttle vector. Environ Mol Mutagen 18:316-321.
28. Kondo S. 1999. I. Evidence that there are threshold effects in risk of radiation. J Nucl Sci Tech 36:1-9.
29. Kovalchuk O, Dubrova YE, Arkhipov A, Hohn B, Kovalchuk I. 2000. Wheat mutation rate after Chernobyl. Nature 407:583-584.
30. Kumar S, Tamura K, Jakobsen IB, Nei M. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244-1245.
31. Matson CW, Rodgers BE, Chesser RK, Baker RJ. 2000. Genetic diversity of Clethrionomys glareolus populations from highly contaminated sites in the Chornobyl region, Ukraine. Environ Toxicol Chem 19:2130-2135.
32. Neel JV. 1999. Two recent radiation-related genetic false alarms: leukemia in West Cumbria, England, and minisatellite mutations in Belarus. Teratology 59:302-306.
33. Redpath JL, Liang D, Taylor TH, Christie C, Elmore E. 2001. The shape of the dose-response curve for radiation-induced neoplastic transformation in vitro: evidence for an adaptive response against neoplastic transformation at low doses of low-LET radiation. Radiat Res 156:700-707.
34. Rodgers BE, Baker RJ. 2000. Frequencies of micronuclei in bank voles from zones of high radiation at Chornobyl, Ukraine. Environ Toxicol Chem 19:1644-1649.
35. Rodgers BE, Chesser RK, Wickliffe JK, Phillips CJ, Baker RJ. 2001a. Subchronic exposure of BALB/c and C57BL/6 strains of Mus musculus to the radioactive environment of the Chornobyl, Ukraine exclusion zone. Environ Toxicol Chem 20:2830-2835.
36. Rodgers BE, Wickliffe JK, Phillips CJ, Chesser RK, Baker RJ. 2001b. Experimental exposure of naïve bank voles (Clethrionomys glareolus) to the Chornobyl, Ukraine, environment: a test of radioresistance. Environ Toxicol Chem 20:1936-1941.
37. Schwartz JL, Jordan R, Sun J, Ma H, Hsie AW. 2000. Dose-dependent changes in the spectrum of mutations induced by ionizing radiation. Radiat Res 153:312-317.
38. Sinclair WK. 1998. The linear no-threshold response: why not linearity? Med Phys 25:285-290.
39. Stiegler GL, Stillwell LC. 1993. Big Blue transgenic mouse lacI mutation analysis. Environ Mol Mutagen 22:127-129.
40. Strauss WM. 1994. Preparation of genomic DNA from mammalian tissue. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K, editors. Current protocols in molecular biology. New York: John Wiley & Sons. p 1:2.2.1-2.2.3.
41. Tani M, Shinmura K, Kohno T, Shiroishi T, Wakana S, Kim S-R, Nohmi T, Kasai H, Takenoshita S, Nagamachi Y, Yokota J. 1998. Genomic structure and chromosomal localization of the mouse Ogg1 gene that is involved in the repair of 8-hydroxyguanine in DNA damage. Mammal Genome 9:32-37.
42. Vilenchik MM, Knudson AG Jr. 2000. Inverse radiation dose-rate effects on somatic and germ-line mutations and DNA damage rates. Proc Natl Acad Sci USA 97:5381-5386.
43. Wickliffe JK, Chesser RK, Rodgers BE, Baker RJ. 2001. Letter to the editor. Mutat Res 478:207-208.
44. Wickliffe JK, Chesser RK, Rodgers BE, Baker RJ. 2002. Assessing the genotoxicity of chronic environmental irradiation using mitochondrial DNA heteroplasmy in the bank vole (Clethrionomys glareolus) at Chornobyl, Ukraine. Environ Toxicol Chem 21:1249-1254.
45. Wickliffe JK, Rodgers BE, Chesser RK, Phillips CJ, Gaschak SP, Baker RJ. 2003. Mitochondrial DNA heteroplasmy in laboratory mice experimentally enclosed in the radioactive Chornobyl environment. Radiat Res (in press).
46. Winegar RA, Lutze LH, Hamer JD, O'Loughlin KG, Mirsalis JC. 1994. Radiation-induced point mutations, deletions and micronuclei in lacI transgenic mice. Mutat Res 307:479-487.
47. Wolff S. 1996. Aspects of the adaptive response to very low doses of radiation and other agents. Mutat Res 358:135-142.
48. Young RR, Rogers BJ, Provost GS, Short JM, Putman DL. 1995. Interlaboratory comparison: liver spontaneous mutant frequency from lambda/lacI transgenic mice (Big Blue®) (II). Mutat Res 327:67-73.