Determination of ephedrine alkaloids and synephrine in dietary supplements by column-switching cation exchange high-performance liquid chromatography with scanning-wavelength ultraviolet and fluorescence detection

Journal of Agricultural and Food Chemistry

Volumn 51, Issue 19, 2003, Pages 5630-5638

  Niemann, Richard A ^a   Gay, Martha L ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Analysis; Cation exchange; Determination; Dietary supplements; Ephedra; Ephedrine alkaloids; Fluorescence; HPLC; Synephrine; UV spectra

Indexed keywords

ACETONE; ALKALOID; EPHEDRINE; EPHEDRINE ALKALOID; METHYLEPHEDRINE; N METHYLPSEUDOEPHEDRINE; NOREPHEDRINE; NORPSEUDOEPHEDRINE; PHENYLEPHRINE; PSEUDOEPHEDRINE; UNCLASSIFIED DRUG;

ARTICLE; CATION EXCHANGE; COLUMN CHROMATOGRAPHY; COLUMN SWITCHING CATION EXCHANGE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; DIET SUPPLEMENTATION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; QUANTITATIVE ANALYSIS; SIGNAL PROCESSING; SPECTROSCOPY; ULTRAVIOLET SPECTROSCOPY;

ALKALOIDS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIETARY SUPPLEMENTS; EPHEDRINE; FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET; SYNEPHRINE;

EPHEDRA;

EID: 0041833743     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf0302052     Document Type: Article

References (12)

1. Haller, C. A.; Benowitz, N. L. Adverse cardiovascular and central nervous system events associated with dietary supplements containing ephedra alkaloids. N. Engl. J. Med. 2000, 343, 1833-1838.
2. Pellati, F.; Benvenuti, S.; Melegari, M.; Firenzuoli, F. Determination of adrenergic agonists from extracts and herbal products of Citrus aurantium L. var. amara by LC. J. Pharm. Biomed. Anal. 2002, 29, 1113-1119.
3. Okamura, N.; Miki, H.; Harada, T.; Yamashita, S.; Masaoka, Y.; Nakamoto, Y.; Tsuguma, M.; Yoshitomi, H.; Yagi, A. Simultaneous determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine in Kampo medicines by high-performance liquid chromatography. J. Pharm. Biomed. Anal. 1999, 20, 363-372.
4. Hashimoto, K.; Yasuda, T.; Ohsawa, K. Determination of synephrine from Chinese medicinal drugs originating from Citrus species by ion-pair high-performance liquid chromatography. J. Chromatogr. 1992, 623, 386-389.
5. Hurlbut, J. A.; Carr, J. R.; Singleton, E. R.; Faul, K. C.; Madson, M. R.; Storey, J. M.; Thomas, T. L. Solid-phase extraction cleanup and liquid chromatography with ultraviolet detection of ephedrine alkaloids in herbal products. J. AOAC Int. 1998, 81, 1121-1127.
6. Gurley, B. J.; Wang, P.; Gardner, S. F. Ephedrine-type alkaloid content of nutritional supplements containing Ephedra sinica (Ma-huang) as determined by high performance liquid chromatography. J. Pharm. Sci. 1998, 87, 1547-1553.
7. He, X.; Lian, L.; Lin, L.; Bernart, M. W. High-performance liquid chromatography-electrospray mass spectrometry in phytochemical analysis of sour orange (Citrus aurantium L.). J. Chromatogr. A 1997, 791, 127-134.
8. Gay, M. L.; White, K. D.; Obermeyer, W. R.; Betz, J. M.; Musser, S. M. Determination of ephedrine-type alkaloids in dietary supplements by LC/MS using a stable-isotope labeled internal standard. J. AOAC Int. 2001, 84, 761-769.
9. Niemann, R. A. Determination of formetanate hydrochloride in selected fruits by coupled-column cation exchange liquid chromatography. J. AOAC Int. 1993, 76, 1362-1368.
10. Gay, M. L.; Niemann, R. A.; Musser, S. M. A multiresidue method for the determination of synephrine and ephedrine-type alkaloids in dietary supplements using a stable-labeled internal standard. 50th ASMS Conference on Mass Spectrometry and Allied Topics (CD-ROM); ASMS: Santa Fe, NM, 2002; Paper 897.
11. Gay, M. L. 2003, to be submitted for publication.
12. Gurley, B. J.; Gardner, S. F.; Hubbard, M. A. Content versus label claims in ephedra-containing dietary supplements. Am. J. Health-Syst. Pharm. 2000, 57, 963-969.