A cytoplasmic region determines single-channel conductance in 5-HT3 receptors

Nature

Volumn 424, Issue 6946, 2003, Pages 321-324

  Kelley, Stephen P ^a   Dunlop, James I ^a   Kirkness, Ewen F ^b   Lambert, Jeremy J ^a   Peters, John A ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b J CRAIG VENTER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; ELECTRIC CONDUCTANCE; ISOMERS; POSITIVE IONS;

CYTOPLASMIC NATURE;

CELLS;

COMPLEMENTARY DNA; DNA; ION CHANNEL; RECEPTOR SUBUNIT; SEROTONIN; SEROTONIN 3 RECEPTOR; SEROTONIN 3A RECEPTOR; SEROTONIN 3B RECEPTOR; UNCLASSIFIED DRUG; ARGININE; CATION; CYSTEINE; HYBRID PROTEIN; PROTEIN SUBUNIT; SEROTONIN RECEPTOR;

BIOLOGY;

ANIMAL CELL; ARTICLE; CHIMERA; CYTOPLASM; IMMUNOFLUORESCENCE; ION CONDUCTANCE; MEMBRANE ELECTROPHYSIOLOGY; NEUROTRANSMISSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PATCH CLAMP; PRIORITY JOURNAL; SEROTONINERGIC SYSTEM; SITE DIRECTED MUTAGENESIS; TORPEDO; AMINO ACID SEQUENCE; CHEMISTRY; ELECTRIC CONDUCTIVITY; ELECTROPHYSIOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE;

ANIMALIA; STAPHYLOCOCCUS PHAGE 3A; TORPEDO;

AMINO ACID SEQUENCE; ARGININE; CATIONS; CONSERVED SEQUENCE; CYSTEINE; CYTOPLASM; ELECTRIC CONDUCTIVITY; ELECTROPHYSIOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; ION CHANNELS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT3; RECOMBINANT FUSION PROTEINS;

EID: 0041806486     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature01788     Document Type: Article

References (29)

1. 3 receptors are membrane ion channels. Nature 339, 706-709 (1989).
2. 3 receptors mediate rapid responses in cultured hippocampus and a clonal cell line. Neuron 1, 615-621 (1988).
3. 3 receptor. Brain Res. 533, 46-52 (1990).
4. Yang, J. Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells. J. Gen. Physiol. 96, 1177-1198 (1990).
5. 3 receptor, a serotonin-gated ion channel. Science 254, 432-437 (1991).
6. 3A). J. Physiol. (Lond.) 507, 653-665 (1998).
7. 3B subunit is a major determinant of serotonin-receptor function. Nature 397, 359-363 (1999).
8. 3B receptor subunit. J. Biol. Chem. 274, 30799-30810 (1999).
9. Hussy, N., Lukas, W. & Jones, K. A. Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: Comparison with native mouse receptors. J. Physiol. (Lond.) 481, 311-323 (1994).
10. Finer-Moore, J. & Stroud, R. M. Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor. Proc. Natl Acad. Sci. USA 81, 155-159 (1984).
11. Miyazawa, A., Fujiyoshi, Y., Stowell, M. & Unwin, N. Nicotinic acetylcholine receptor at 4.6 A resolution: Transverse tunnels in the channel wall. J. Mol. Biol. 288, 765-786 (1999).
12. 3 receptors: The role of oligomerization and chaperone proteins. Mol. Cell. Neurosci. 21, 38-50 (2002).
13. 3 receptor stably expressed in HEK 293 cells. Br. J. Pharmacol. 114, 1211-1221 (1995).
14. 3 receptors in rodents. J. Neurochem. 75, 240-247 (2002).
15. Bouzat, C., Bren, N. & Sine, S. M. Structural basis of the different gating kinetics of fetal and adult acetylcholine receptors. Neuron 13, 1395-1402 (1994).
16. Akk, G. & Steinbach, J. H. Structural elements near the C-terminus are responsible for changes in nicotinic receptor gating kinetics following patch excision. J. Physiol. (Lond.) 527, 405-417 (2000).
17. Yu, X. M. & Hall, Z. W. A sequence in the main cytoplasmic loop of the alpha subunit is required for assembly of mouse muscle nicotinic acetylcholine receptor. Neuron 13, 247-255 (1994).
18. Imoto, K. et al. Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance. Nature 335, 645-648 (1988).
19. 3 receptor pore revealed by substituted cysteine accessibility mutagenesis. J. Biol. Chem. 276, 42035-42042 (2001).
20. Panicker, S., Cruz, H., Arrabit, C. & Slesinger, P. A. Evidence for a centrally located gate in the pore of a serotonin-gated ion channel. J. Neurosci. 22, 1629-1639 (2002).
21. 3A receptor from cationic to anionic reveals a conserved feature of the ligand-gated ion channel superfamily. J. Biol. Chem. 276, 10977-10983 (2001).
22. Unwin, N. The Croonian Lecture 2000. Nicotinic acetylcholine receptor and the structural basis of fast synaptic transmission. Phil. Trans. R. Soc. Lond. B 355, 1813-1829 (2000).
23. Sokolova, O., Kolmakova-Partensky, L. & Grigorieff, N. Three-dimensional structure of a voltage-gated potassium channel at 2.5 nm resolution. Structure 9, 215-220 (2001).
24. + channel. Biochemistry 39, 10347-10352 (2000).
25. Bass, R. B., Strop, P., Barclay, M. & Rees, D. C. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science 298, 1582-1587 (2002).
26. Sato, C. et al. The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities. Nature 409, 1047-1051 (2001).
27. Higgins, M. K., Weitz, D., Warne, T., Schertler, G. F. & Kaupp, U. B. Molecular architecture of a retinal cGMP-gated channel: The arrangement of the cytoplasmic domains. EMBO J. 21, 2087-2094 (2002).
28. Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning - A Laboratory Manual, 2nd edn (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
29. 2+. J. Biol. Chem. 278, 712-717 (2002).