High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli

Protein Expression and Purification

Volumn 27, Issue 1, 2003, Pages 150-157

  Hiszczyńska Sawicka, Elz̀bieta ^a   Brillowska Da̧browska, Anna ^a   Da̧abrowski, Sławomir ^a   Pietkiewicz, Halina ^b   Myjak, Przemysław ^b   Kur, Józef ^a  

^a GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

^b MEDICAL UNIVERSITY OF GDANSK   (Poland)

Author keywords

ELISA; Fusion protein; Metal affinity chromatography; Serological detection; Toxoplasma gondii antigens; Western blot

Indexed keywords

ESCHERICHIA COLI; TOXOPLASMA GONDII;

GRA1 ANTIGEN; PARASITE ANTIGEN;

ANIMAL; ARTICLE; BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; TOXOPLASMA;

ANIMALS; ANTIGENS, PROTOZOAN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; TOXOPLASMA;

EID: 0041783103     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00593-4     Document Type: Article

References (50)

1. A.M. Johnson, The antigenic structure of Toxoplasma gondii: a review, Pathology 17 (1985) 9-19.
2. R. McLeod, D. Mack, C. Brown, Toxoplasma gondii: new advances in cellular an molecular biology, Exp. Parasitol. 72 (1991) 109-121.
3. J. Smith, A ubiquitous intracellular parasite: the cellular biology of Toxoplasma gondii, Int. J. Parasitol. 25 (1995) 1301-1309.
4. S.Y. Wong, J.S. Remington, Biology of Toxoplasma gondii, AIDS 7 (1993) 299-316.
5. J.M. Pinon, C. Chemla, I. Villena, F. Foundrinier, D. Aubert, D. Puygauthier-Toubas, B. Leroux, D. Dupouy, C. Quereux, M. Talmud, T. Trenque, G. Potron, M. Pluot, G. Remy, A. Bonhomme, Early neonatal diagnosis of congenital toxoplasmosis: value of comparative enzyme-linked immunofiltration assay immunological profiles and anti-Toxoplasma gondii immunoglobulin M (IgM) or IgA immunocapture and implication for postnatal therapeutic strategies, J. Clin. Microbiol. 34 (1996) 579-583.
6. F.G. Araujo, J.S. Remington, Toxoplasmosis in immunocompromised patients, Eur. J. Clin. Microbiol. 6 (1987) 1-12.
7. D. Aubert, F. Foudrinier, I. Villena, J.M. Pinon, M.F. Biava, E. Renoult, PCR for diagnosis and follow-up of two cases of disseminated toxoplasmosis after kidney grafting, J. Clin. Microbiol. 34 (1996) 1347.
8. W.T. Hofgartner, S.R. Swanzy, R.M. Bacina, J. Condon, M. Gupta, P.E. Matlock, D.L. Bergeron, J.J. Plorde, T.R. Fritsche, Detection of immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii: evaluation of four commercial immunoassay systems, J. Clin. Microbiol. 35 (1997) 3313-3315.
9. P.A. Jenum, B. Stray-Pedersen, Development of specific immunoglobulins G, M, and A following primary Toxoplasma gondii infection in pregnant women, J. Clin. Microbiol. 36 (1998) 2907-2913.
10. O. Liesenfeld, C. Press, R. Flanders, R. Ramirez, J.S. Remington, Study of Abbott IMx system for detection of immunoglobulin G and immunoglobulin M toxoplasma antibodies: value of confirmatory testing for diagnosis of acute toxoplasmosis, J. Clin. Microbiol. 34 (1996) 2526-2530.
11. O. Liesenfeld, C. Press, J.G. Montoya, R. Gill, J.L. Isaac-Renton, K. Hedman, J.S. Remington, False-positive results in immunoglobulin M (IgM) toxoplasma antibody tests and importance of confirmatory testing: the platelia toxo IgM test, J. Clin. Microbiol. 35 (1997) 174-178.
12. S. Singh, N. Singh, S.N. Dwivedi, Evaluation of seven commercially available ELISA kits for serodiagnosis of acute toxoplasmosis, Indian J. Med. Res. 105 (1997) 103-107.
13. M. Wilson, J.S. Remington, C. Clavet, G. Varney, C. Press, D. Ware, Evaluation of six commercial kits for the detection of human immunoglobulin M antibodies to Toxoplasma gondii. The FDA toxoplasmosis ad hoc working group, J. Clin. Microbiol. 35 (1997) 3112-3115.
14. F. Derouin, C. Rabian-Herzog, A. Sulahian, Longitudinal study of the specific humoral and cellular response to Toxoplasma gondii in a patient with acquired toxoplasmosis, J. Clin. Lab. Immunol. 30 (1989) 97-102.
15. J. Hafid, R.T.M. Sung, Z.Y. Akono, B. Pozetto, J. Jaubert, H. Raberin, M. Jana, Toxoplasma gondii antigens: analysis by SDS-PAGE and immunoblotting; relation with circulating antigens, Eur. J. Protistol. 26 (1991) 303-307.
16. J. Huskinson, P.N. Stepick-Biek, F.G. Araujo, P. Thulliez, Y. Suzuki, J.S. Remington, Toxoplasma antigens recognized by immunoglobulin G subclasses during acute and chronic infection, J. Clin. Microbiol. 27 (1989) 2031-2038.
17. J. Huskinson, P. Thulliez, J.S. Remington, Toxoplasma antigens recognized by human immunoglobulin A antibodies, J. Clin. Microbiol. 28 (1990) 2632-2636.
18. E.G. Lindenschmidt, Demonstration of immunoglobulin M class antibodies to Toxoplasma gondii antigenic component p35000 by enzyme-linked antigen immunosorbent assay, J. Clin. Microbiol. 24 (1986) 1045-1049.
19. I.L. Moir, M.M. Davidson, D.O. Ho-Yen, Comparison of IgG antibody profiles by immunoblotting in patients with acute and previous Toxoplasma gondii infection, J. Clin. Pathol. 44 (1991) 569-572.
20. J.M. Pinon, F. Foudrinier, G. Mougeot, C. Marx, D. Aubert, O. Toupance, G. Niel, M. Danis, P. Camerlynck, G. Remy, J. Frottier, D. Jolly, M.H. Bessieres, D. Richard-Lenoble, A. Bonhomme, Evaluation of risk and diagnostic value of quantitative assays for anti-Toxoplasma gondii immunoglobulin A (IgA), IgE, and IgM and analytical study of specific IgG immunodeficient patients, J. Clin. Microbiol. 33 (1995) 878-884.
21. I. Potasman, F.G. Araujo, G. Desmonts, J.S. Remington, Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection, J. Infect. Dis. 154 (1986) 650-657.
22. S.D. Sharma, J. Mullenax, F.G. Araujo, H.A. Erlich, J.S. Remington, Western blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies, J. Immunol. 131 (1983) 977-983.
23. C. Verhofstede, P. Van Gelder, M. Rabaey, The infection-stage-related IgG response to Toxoplasma gondii studied by immunoblotting, Parasitol. Res. 74 (1988) 516-520.
24. L.M. Weiss, S.A. Udem, H. Tanowitz, M. Wittner, Western blot analysis of the antibody response of patients with AIDS and Toxoplasma encephalitis: antigenic diversity among Toxoplasma strains, J. Infect. Dis. 157 (1988) 7-13.
25. S.F. Parmley, G.D. Sgarlato, J. Mark, J.B. Prince, J.S. Remington, Expression, characterization, and serologic reactivity of recombinant surface antigen P22 of Toxoplasma gondii, J. Clin. Microbiol. 30 (1992) 1127-1133.
26. J.B. Prince, K.L. Auer, J. Huskinson, S.F. Parmley, F.G. Araujo, J.S. Remington, Cloning, expression, and cDNA sequence of surface antigen P22 from Toxoplasma gondii, Mol. Biochem Parasitol. 43 (1990) 97-106.
27. J.L. Burg, D. Perelman, L.H. Kasper, P.L. Ware, J.C. Boothroyd, Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii, J. Immunol. 141 (1988) 3584-3591.
28. D. Harning, J. Spenter, A. Metsis, J. Vuust, E. Petersen, Recombinant Toxoplasma gondii surface antigenl (P30) expressed in Escherichia coli is recognized by human Toxoplasma-specific immunoglobulin M (IgM) and IgG antibodies, Clin. Diagn. Lab. Immunol. 3 (1996) 355-357.
29. K. Kim, R. Bülow, J. Kampmeier, J.C. Boothroyd, Conformationally appropriate expression of the Toxoplasma antigen SAG1 (p30) in CHO cells, Infect. Immun. 62 (1994) 203-209.
30. M.F. Cesbron-Delauw, B. Guy, G. Torpier, R.J. Pierce, G. Lenzen, J.Y. Cesbron, H. Charif, P. Lepage, F. Darcy, J.P. Lecocq, A. Capron, Molecular characterization of a23 kilodalton major antigen secreted by Toxoplasma gondii, Proc. Natl. Acad. Sci. USA 86 (1989) 7537-7541.
31. A. Murray, C. Mercier, A. Decoster, L. Lecordier, A. Capron, M.F. Cesbron-Delauw, Multiple B-cell epitopes in a recombinant GRA2 secreted antigen of Toxoplasma gondii, Appl. Parasitol. 34 (1993) 235-244.
32. J.B. Prince, F.G. Araujo, J.S. Remington, J.L. Burg, J.C. Boothroyd, S.D. Sharma, Cloning of cDNAs encoding a 28 kDa antigen of Toxoplasma gondii, Mol. Biochem. Parasitol. 34 (1989) 3-14.
33. A. Bonhomme, G.T. Maine, A. Beorchia, H. Burlet, D. Aubert, I. Villena, J. Hunt, L. Chovan, L. Howard, S. Brojanac, M. Sheu, J. Tyner, M. Pluot, J.M. Pinon, Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of Toxoplasma gondii, J. Histochem. Cytochem. 46 (1998) 1411-1421.
34. H.G. Fischer, S. Stachelhaus, M. Sahm, H.M. Meyer, G. Reichmann, GRA7, an excretory 29kDa Toxoplasma gondii dense granule protein released by infected host cells, Mol. Biochem. Parasitol. 91 (1998) 251-262.
35. D. Jacobs, J.F. Dubremetz, A. Loyens, F. Bosman, E. Saman, Identification and heterologous expression of a new dense granule protein (GRA7) from Toxoplasma gondii, Mol. Biochem Parasitol. 91 (1998) 237-249.
36. L. Lecordier, I. Moleon-Borodowsky, J.F. Dubremetz, B. Tourvieille, C. Mercier, D. Deslée, A. Capron, M.F. Cesbron-Delauw, Characterization of a dense granule antigen of Toxoplasma gondii (GRA6) associated to the network of the parasitophorous vacuole, Mol. Biochem. Parasitol. 70 (1995) 85-94.
37. A. Redlich, W.A. Müller, Serodiagnosis of acute toxoplasmosis using a recombinant form of the dense granule antigen GRA6 in an enzyme-linked immunosorbent assay, Parasitol. Res. 84 (1998) 700-706.
38. M.N. Mevelec, T. Chardes, O. Mercereau-Puijalon, I. Bourguin, A. Achbarou, J.F. Dubremetz, D. Bout, Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein recognized by mucosal IgA antibodies, Mol. Biochem Parasitol. 56 (1992) 227-238.
39. A.M. Tenter, A.M. Johnson, Recognition of recombinant Toxoplasma gondii antigens by human sera in an ELISA, Parasitol. Res. 77 (1991) 197-203.
40. V. Martin, M. Arcavi, G. Santillan, M.R.R Amendoeira, E. De Souza Neves, G. Griemberg, E. Guarnera, J.C. Garberi, S.O. Angel, Detection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii ROP2 protein, Clin. Diagn. Lab. Immunol. 5 (1998) 627-631.
41. R. Saavedra, F. De Meuter, J.L. Decourt, P. Hérion, Human T cell clone identified a potentially protective 54 kDa protein antigen of Toxoplasma gondii cloned and expressed in Escherichia coli, J. Immunol. 147 (1991) 1975-1982.
42. V. Van Gelder, F. Bosman, F. De Meuter, H. Van Heuverswyn, P. Hérion, Serodiagnosis of toxoplasmosis by using a recombinant form of the 54-kDa rhoptry antigen expressed in Escherichia coli, J. Clin. Microbiol. 31 (1993) 9-15.
43. S. Knapp, R. Zlegelmaler, H. Küpper, Toxoplasma gondii antigens, their production and application. European Patent Application 0431541A2 (1991).
44. P.N. Ossorio, J.D. Schwartzman, J.C. Boothroyd, A Toxoplasma gondii rhoptry protein associated with host cell penetration has unusual charge asymmetry, Mol. Biochem. Parasitol. 50 (1992) 1-16.
45. S. Nockemann, H. Długońska, B. Henrich, A. Kitzerow, W. Däubener, Expression, characterization and serological reactivity of a 41kDa excreted-secreted antigen (ESA) from Toxoplasma gondii, Mol. Biochem. Parasitol. 97 (1998) 109-121.
46. A.M. Johnson, S. Illana, Cloning of Toxoplasma gondii gene fragments encoding diagnostic antigens, Gene 99 (1991) 127-132.
47. D. Aubert, G.T. Maine, I. Villena, J.C. Hunt, L. Howard, M. Sheu, S. Brojanac, L.E. Chovan, S.F. Nowlan, J.M. Pinon, Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin A in human sera by enzyme immunoassay, J. Clin. Microbiol. 38 (2000) 1144-1150.
48. S. Li, G. Galvan, F.G. Araujo, Y. Suzuki, J.S. Remington, S. Parmley, Serodiagnosis of recently acquired Toxoplasma gondii infection using an enzyme-linked immunosorbent assay with a combination of recombinant antigens, Clin. Diagn. Lab. Immunol. 7 (2000) 781-787.
49. A.M. Johnson, H. Roberts, A.M. Tenter, Evaluation of a recombinant antigen ELISA for the diagnosis of acute toxoplasmosis and comparison with traditional ELISAs, J. Med. Microbiol. 37 (1992) 404-409.
50. S. Da̧browski, J. Kur, Cloning and expression in Escherichia coli of the recombinant His-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei, Protein Expr. Purif. 14 (1998) 131-138.