Identification and heterologous expression of a new dense granule protein (GRA7) from Toxoplasma gondii

Molecular and Biochemical Parasitology

Volumn 91, Issue 2, 1998, Pages

  Jacobs, Dirk ^a   Dubremetz, Jean François ^b   Loyens, Anne ^b   Bosman, Fons ^a   Saman, Eric ^a  

^a INNOGENETICS   (Belgium)

^b Unité 42   (France)

Author keywords

Dense granule; Expression library; Toxoplasma gondii; Ultrastructure

Indexed keywords

DENSE GRANULE PROTEIN; PARASITE ANTIGEN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; GRANULE CELL; IMMUNOBLOTTING; IMMUNOELECTRON MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FAMILY; SEQUENCE ANALYSIS; TOXOPLASMA GONDII;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, PROTOZOAN; ANTIGENS, PROTOZOAN; BASE SEQUENCE; BLOTTING, WESTERN; CERCOPITHECUS AETHIOPS; CYTOPLASMIC GRANULES; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT ANTIBODY TECHNIQUE; GENES, PROTOZOAN; HUMANS; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; TOXOPLASMA; VERO CELLS;

TOXOPLASMA GONDII;

EID: 0032521225     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(97)00204-1     Document Type: Article

References (37)

1. [1] Remington JS, Desmonts G. Toxoplasmosis. In: Remington JS, Klein JO, editors. Infectious Diseases of the Fetus and Newborn Infant. 2nd ed. Philadelphia: W.B. Saunders, 1990: 89-195
2. [2] Luft BJ, Brooks RG, Conley FK, McCabe RE, Remington JS. Toxoplasma encephalitis in patients with AIDS. J Am Med Assoc 1984;252:913-7.
3. [3] Sam-Yellowe TY. Rhoptry organelles of the Apicomplexa: Their role in host cell invasion and intracellular survival. Parasitol Today 1996;12:308-16.
4. [4] Saffer LD, Mercereau-Puijalon O, Dubremetz J-F, Schwartzman JD. Localisation of a Toxoplasma gondii rhoptry protein by immunoelectron microscopy during and after host cell penetration. J Protozool 1992;39:526-30.
5. [5] Beckers CJM, Dubremetz J-F, Mercereau-Puijalon O, Joiner KA. The Toxoplasma gondii rhoptry protein ROP2 is inserted into the parasitophorous vacuole membrane, surrounding the intracellular parasite, and is exposed to the host cell cytoplasm. J Cell Biol 1994;127:947-61.
6. [6] Cesbron-Delauw M-F. Dense granule organelles of Toxoplasma gondii: their role in the host-parasite relationship. Parasitol Today 1994;10:327-8.
7. [7] De Carvalho L, De Souza W. Cytochemical localization of plasmamembrane markers during interiorisation of tachyzoites of Toxoplasma gondii by macrophages. J Protozool 1989;36:164-70.
8. [8] Sibley LD, Weidner E, Krahenbuhl JL. Phagosome acidification blocked by intracellular Toxoplasma gondii. Nature 1985;315:416-9.
9. [9] Joiner KA, Fuhrman SA, Miettinen HM, Kasper LH, Mellman I. Toxoplasma gondii: fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. Science 1990;249:641-6.
10. [10] Torpier G, Charif H, Darcy F, Liu J, Darde M-L, Capron A. Toxoplasma gondii: Differential location of antigens secreted from encysted bradyzoites. Exp Parasitol 1993;77:13-22.
11. [11] Cesbron-Delauw M-F, Guy B, Torpier G, Pierce RJ, Lenzen G, Gesbron JY, Charif H, Lepage P, Darcy F, Lecocq JP, Capron A. Molecular charac tersation of a 23-kilodalton major antigen secreted by Toxoplasma gondii. Proc Natl Acad Sci USA 1989;86:7537-41.
12. [12] Prince JB, Araujo FG, Remington JS, Burg JL, Boothroyd JC, Sharma SD. Cloning of cDNAs encoding a 28 kilodalton antigen of Toxoplasma gondii. Mol Biochem Parasitol 1989;34:3-13.
13. [13] Parmley SF, Sgarlato GD, Remington JS. Genomic and corrected cDNA sequence of the P28 gene from Toxoplasma gondii. Mol Biochem Parasitol 1993;57:161-6.
14. [14] Mercier C, Lecordier L, Darcy F, Deslee D, Murray A, Tourvieille B, Maes P, Capron A, Cesbron-Delauw M-F. Molecular characterisation of a dense granule antigen (GRA 2) associated with the network of the parasitophorous vacuole in Toxoplasma gondii. Mol Biochem Parasitol 1993;58:71-82.
15. [15] Mevelec M-N, Chardes T, Mercereau-Puijalon O, Bourgin I, Achbarou A, Dubremetz J-F, Bout D. Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies. Mol Biochem Parasitol 1992;56:227-38.
16. [16] Lecordier L, Moleon-Borodowsky I, Dubremetz J-F, Tourvieille B, Mercier C, Deslee D, Capron A, Cesbron-Delauw M-F. Characterisation of a dense granule antigen of Toxoplasma gondii (GRA6) associated to the network of the parasitophorous vacuole. Mol Biochem Parasitol 1995;70:85-94.
17. [17] Bermudes D, Dubremetz J-F, Achbarou A, Joiner KA. Cloning of a cDNA encoding the dense granule protein GRA3 from Toxoplasma gondii. Mol Biochem Parasitol 1994;68:247-57.
18. [18] Lecordier L, Mercier C, Torpier G, Tourvieille B, Darcy F, Liu JL, Maes P, Tartar A, Capron A, Cesbron-Delauw M-F. Molecular structure of a Toxoplasma gondii dense granule antigen (GRA5) associated with the parasitophorous vacuole membrane. Mol Biochem Parasitol 1993;56:143-54.
19. [19] Bermudes D, Ran Peck K, Afifi Afifi M, Beckers CJM, Joiner KA. Tandemly repeated genes encode nucleoside triphosphate hydrolase isoforms secreted into the parasitophorous vacuole of Toxoplasma gondii. J Biol Chem 1994;269:29252-60.
20. [20] Sibley LD, Niesman IR, Asai T, Takeuchi T. Toxoplasma gondii: secretion of a potent nucleoside triphosphate hydrolase into the parasitophorous vacuole. Exp Parasitol 1994;79:301-11.
21. [21] Saavedra R, De Meuter F, Herion P. Monoclonal antibodies identify new Toxoplasma gondii soluble antigens. Hybridoma 1990;9:453-63.
22. [22] Maniatis T, Fritsch EF, Sambrook J. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory, 1982.
23. [23] Saavedra R, De Meuter F, Decourt JL, Herion P. Human T-cell clone identifies a potentially protective 54-kDa protein antigen of Toxoplasma gondii cloned and expressed in Escherichia coli. J Immunol 1991;147:1975-82.
24. [24] Huynh TV, Young RA, Davis RW. Constructing and screening cDNA libraries in λgt10 and λgt11. In: Glover DM, editor. DNA cloning, vol. I. Oxford, UK: IRL Press, 1985:49-78.
25. [25] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5.
26. [26] Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
27. [27] Blake MS, Johnston KH, Russell-Jones GJ, Gotschlich EC. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem 1984;136:175-9.
28. [28] Sanger F, Nicklen S, Coulson AS. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 1977;74:5463-7.
29. [29] Gilot P, De Kesel M, Machtelinckx L, Coene M, Cocito C. Isolation and sequencing of the gene coding for an antigenic 34-kilodalton protein of Mycobacterium paratuberculosis. J Bacteriol 1993;175:4930-5.
30. [30] Hochuli E, Bannwarth H, Doebeli R, Gentz R, Stueber D. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsor bent. Biotechnology 1988;6:1321-5.
31. [31] Remaut E, Tsao H, Fiers W. Improved plasmid vectors with a thermoinducible expression and temperature regulated runaway replication. Gene 1983;22:103-13.
32. [32] Van Gelder P, Bosman F, De Meuter F, Van Heuverswyn H, Hérion P. Serodiagnosis of toxoplasmosis by using a recombinant form of the 54-kilodalton rhoptry antigen expressed in Escherichia coli. J Clin Microbiol 1993;31:9-15.
33. [33] Hérion P, Hernandez-Pando R, Dubremetz J-F, Saavedra R. Subcellular localisation of the 54-kDa antigen of Toxoplasma gondii. J Parasitol 1993;79:216-22.
34. [34] Seeber F. Consensus sequence of translational initiation sites from Toxo plasma gondii genes. Parasitol Res 1997;83:309-11.
35. [35] Kyte J, Doolittle RF. A simple method for displaying the hydrophobic character of a protein. J Mol Biol 1982;157:105-32.
36. [36] Achbarou A, Mercereau-Puijalon O, Sadak A, Fortier B, Leriche MA, Camus D, Dubremetz JF. Differential targeting of dense granule proteins in the parasitophorous vacuole of Toxoplasma gondii. Parasitology 1991;103:321-9.
37. [37] Dubremetz JF, Achbarou A, Bermudes D, Joiner KA. Kinetics and pattern of organelle exocytosis during Toxoplasma/host-cell interaction. Parasitol Res 1993; 79:402-8.