Subsite specificity (S3, S2, S1′, S2′ and S3′) oligopeptidase B from Trypanosoma cruzi and Trypanosoma brucei using fluorescent quenched peptides: Comparative study and identification of specific carboxypeptidase activity

Biochemical Journal

Volumn 373, Issue 3, 2003, Pages 933-939

  Hemerly, Jefferson P ^a   Oliveira, Vitor ^a   Del Nery, Elaine ^a   Morty, Rory E ^b,c   Andrews, Norma W ^b   Juliano, Maria A ^a   Juliano, Luiz ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Arginyl hydrolase; Chagas disease; Prolyl oligopeptidase; Sleeping sickness

Indexed keywords

AMINO ACIDS; FLUORESCENCE; HYDROLYSIS; QUENCHING;

SUBSITE SPECIFICITY;

ENZYMES;

ALANINE; ALANYLGLYCYLGLYCYLARGINYLGLYCYLALANYLGLUTAMINE; AMINO ACID; AMINOPEPTIDASE B; ANTHRANILIC ACID; ARGININE; CARBOXYPEPTIDASE; GLUTAMINE; HYDROGEN; KININOGEN; N (2,4 DINITROPHENYL)ETHYLENEDIAMINE; OLIGOPEPTIDASE B; PEPTIDASE; PEPTIDE; PROTOZOAL PROTEIN; RECOMBINANT ENZYME; SERINE; SYNTHETIC PEPTIDE; THREONINE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME SUBUNIT; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS; SEQUENCE HOMOLOGY; SPECIES COMPARISON; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

AMINO ACID SEQUENCE; ANIMALS; CARBOXYPEPTIDASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLUORESCENT DYES; KINETICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMA CRUZI;

PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

EID: 0041566922     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030342     Document Type: Article

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