Determination of the InvE binding site required for expression of IpaB of the Shigella sonnei virulence plasmid: Involvement of a ParB BoxA-like sequence

Journal of Bacteriology

Volumn 185, Issue 17, 2003, Pages 5158-5165

  Taniya, Takayuki ^a,b   Mitobe, Jiro ^a   Nakayama, Shu Ichi ^a   Mingshan, Qi ^c   Okuda, Kenji ^b   Watanabe, Haruo ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b YOKOHAMA CITY UNIVERSITY   (Japan)

^c School of Dental Medicine at SUNY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; PROTEIN INVE; PROTEIN PARB; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; DNA BINDING; ESCHERICHIA COLI K 12; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; IPAB GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SHIGELLA SONNEI; TRANSCRIPTION INITIATION;

BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS (GENETICS); SHIGELLA SONNEI; TRANSCRIPTION, GENETIC; VIRAL PROTEINS; VIRULENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ESCHERICHIA COLI K12; NEGIBACTERIA; SHIGELLA; SHIGELLA SONNEI;

EID: 0041387465     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.17.5158-5165.2003     Document Type: Article

References (38)

1. Adler, B., C. Sasakawa, N. Okada, T. Tobe, S. Makino, K. Komatsu, and M. Yoshikawa. 1989. A dual transcriptional activation system for the 230kb plasmid genes coding for virulence-associated antigens of Shigella flexneri. Mol. Microbial. 3:627-635.
2. Allaoui, A., P. J. Sansonetti, and C. Parsot. 1993. MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri Ipa invasins. Mol. Microbiol. 7:59-1768.
3. Andrews, G. P., A. E. Hromockyj, C. Coker, and A. T. Maurelli. 1991. Two novel virulence loci, mxiA and mxiB, in Shigella flexneri 2a facilitate excretion of invasion plasmid antigens. Infect. Immun. 59:1997-2005.
4. Beloin, C., S. McKenna, and C. J. Dorman. 2002. Molecular dissection of VirB, a key regulator of the virulence cascade of Shigella flexneri. J. Biol. Chem. 277:15333-15344.
5. Bignell, C., and C. M. Thomas. 2001. The bacterial ParA-ParB partitioning proteins. J. Biotechnol. 91:1-34.
6. Chomczynsld, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
7. Datsenko, K. A., and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 with PCR products. Proc. Natl. Acad. Sci. USA 97:6640-6645.
8. Dorman, C. J., and M. E. Porter. 1998. The Shigella virulence gene regulatory cascade: a paradigm of bacterial gene control mechanisms. Mol. Microbiol. 29:677-684.
9. Funnell, B. E., and L. Gagnier. 1993. The P1 plasmid partition complex at parS. II. Analysis of ParB protein binding activity and specificity. J. Biol. Chem. 268:3616-3624.
10. Hale, T. L., P. J. Sansonetti, P. A. Schad, S. Austin, and S. B. Formal. 1983. Characterization of virulence plasmids and plasmid-associated outer membrane proteins in Shigella flexneri, Shigella sonnei, and Escherichia coli. Infect. Immun. 40:340-350.
11. Hale, T. L., E. V. Oaks, and S. B. Formal. 1985. Identification and antigenic characterization of virulence-associated, plasmid-coded protein of Shigella spp. and enteroinvasive Escherichia coli. Infect. Immun. 50:620-629.
12. Hale, T. L. 1991. Genetic basis of virulence in Shigella species. Microbiol. Rev. 55:206-224.
13. Ito, K., T. Nakajima, T. Sasaki, and H. Watanabe. 1991. Localization of IpaB protein in Escherichia coli K-12 MC1061 strain carrying Shigella sonnei form I plasmid pSS120. Microbiol. Immunol. 35:335-341.
14. Kato, J., K. Ito, A. Nakamura, and H. Watanabe. 1989. Cloning of regions required for contact hemolysis and entry into LLC-MK2 cells from Shigella sonnei form I plasmid: virF is a positive regulator gene for these phenotypes. Infect. Immun. 57:1391-1398.
15. Kwong, S. M., C. C. Yeo, and C. L. Poh. 2001. Molecular analysis of the pRA2 partitioning region: ParB autoregulates parAB transcription and forms a nucleoprotein complex with the plasmid partition site parS. Mol. Microbiol. 40:621-633.
16. Makino, S., C. Sasakawa, K. Kamata, and M. Yoshikawa. 1986. A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in Shigella flexneri 2a. Cell 46:551-555.
17. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
18. Manoil, C., and J. Beckwith. 1985. TnphoA: a transposon probe for protein export signals. Proe. Natl. Acad. Sci. USA 82:8129-8133.
19. Maurelli, A., T., B. Blackmon, and R. Curtiss III. 1984. Temperature-dependent expression of virulence genes in Shigella species. Infect. Immun. 43:195-201.
20. Maurelli, A., T., B. Baundry, T. L. Hale, and P. J. Sansonetti. 1985. Cloning of plasmid DNA sequence involved in invasion HeLa cells by Shigella flexneri 2a. Infect. Immun. 49:164-171.
21. Menard, R., P. J. Sansonetti, and C. Parsot. 1993. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J. Bacteriol. 175:5899-5906.
22. Menard, R., P. J. Sansonetti, C. Parsot, and T. Vasselon. 1994. Extracellular association and cytoplasmic partition of IpaB and IpaC invasins of Shigella flexneri. Cell 79:515-535.
23. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Parsot, C. 1994. Shigella flexneri: genetics of entry and intercellular dissemination in epithelial cells. Curr. Top. Microbiol. Immunol. 192:217-241.
25. Radnedge, L., M. A. Davis, and S. J. Austin. 1996. P1 and P7 plasmid partition: ParB protein bound to its partition site makes a separate discriminator contact with the DNA that determines species specificity. EMBO J. 15:1155-1162.
26. Sakai, T., C. Sasakawa, and M. Yoshikawa. 1988. Expression of four virulence antigens of Shigella flexneri is positively regulated at the transcriptional level by the 30kDa virF protein. Mol. Microbiol. 2:589-597.
27. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
28. Sansonetti, P. J., D. J. Kopecko, and S. B. Formal. 1982. Involvement of a plasmid in the invasive ability of Shigellaflexneri. Infect. Immun. 35:852-860.
29. Sasakawa, C., K. Kamata, T. Tobe, T. Suzuki, and M. Yoshikawa. 1988. Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella flexneri 2a. J. Bacteriol. 170:2480-2484.
30. Sasakawa, C., J. M. Buysse, and H. Watanabe. 1992. The large virulence plasmid of Shigella. Curr. Top. Microbiol. Immunol. 180:21-44.
31. Sasakawa, C., K. Komatsu, T. Tobe, T. Suzuki, and M. Yoshikawa. 1993. Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a. J. Bacteriol. 175:2334-2346.
32. Sharma, R. C., and R. T. Schimke. 1996. Preparation of electrocompetent E. coli with salt-free growth medium. BioTechniques 20:42-44.
33. Smith, D. B., and K. S. Johnson. 1988. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40.
34. Stachel, S. E., G. An, C. Flores, and E. W. Nester. 1985. A Tn3-lacZ transposon for the random generation of β-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 4:891-898.
35. Surtees, J. A., and B. E. Funnell. 2001. The DNA binding domains of P1 ParB and the architecture of the P1 plasmid partition complex. J. Biol. Chem. 276:12385-12394.
36. Venkatesan, M., J. M. Buysse, and D. J. Kopecko. 1988. Characterization of invasion plasmid antigen (ipaBCD) gene from Shigella flexneri: DNA sequence analysis and control of gene expression. Proc. Natl. Acad. Sci. USA 85:9317-9321.
37. Watanabe, H., E. Arakawa, K.-I. Ito, J.-I. Kato, and A. Nakamura. 1990. Genetic analysis of an invasion region by use of a Tn3-lac transposon and identification of a second positive regulator gene, invE, for cell invasion of Shigella sonnei: significant homology of InvE with ParB of plasmid P1. J. Bacteriol. 172:619-629.
38. Zychlinsky, A., B. Kenny, R. Menard, M. C. Prevost, I. B. Holland, and P. J. Sansonetti. 1994. IpaB mediates macrophage apoptosis induced by Shigella flexneri. Mol. Microbiol. 11:619-627.