Efficient technique for immortalization of murine microglial cells relevant for studies in murine models of multiple sclerosis

Journal of Neuroscience Methods

Volumn 128, Issue 1-2, 2003, Pages 33-43

  Olson, Julie K ^a   Zamvil, Scott S ^b   Miller, Stephen D ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Antigen presentation; Immortalization; Microglial cell line; Theiler's virus

Indexed keywords

VIRUS LARGE T ANTIGEN;

ANIMAL CELL; ANTIGEN PRESENTING CELL; ARTICLE; CELL ACTIVATION; CELL CULTURE; CELL DIVISION; CELL IMMORTALIZATION; CELL LINE; INFECTION; MICROGLIA; MOUSE; MULTIPLE SCLEROSIS; NEWBORN; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE;

EID: 0041387457     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(03)00145-6     Document Type: Article

References (28)

1. Aloisi F. Immune function of microglia. Glia. 36:2001;165-179.
2. Barron K.D. The microglial cell. A historical review. J. Neurol. Sci. 134:1995;57-68.
3. Bernard C.C., de Rosbo N.K. Immunopathological recognition of autoantigens in multiple sclerosis. Acta Neurol. 13:1991;171-178.
4. Blasi E., Bartuzzi R., Bocchini V., Mazzolla R., Bistoni F. Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J. Neuroimmunol. 27:1990;229-237.
5. Brostoff S.W., Reuter W., Hichens M., Eylar E.H. Specific cleavage of the A1 protein from myelin with cathepsin D. J. Biol. Chem. 249:1974;559-567.
6. Cuadros M.A., Navascues J. Early origin and colonization of the developing central nervous system by microglial precursors. Prog. Brain Res. 132:2001;51-59.
7. de Rosbo N.K., Hoffman M., Mendel I., Yust I., Kaye J., Bakimer R., Flechter S., Abramsky O., Milo R., Kami A., Ben-Nun A. Predominance of the autoimmune response to myelin oligodendrocyte glycoprotein (MOG) in multiple sclerosis: reactivity to the extracellular domain of MOG is directed against three main regions. Eur. J. Immunol. 27:1997;3059-3069.
8. Desimone R., Giampaolo A., Giometto B., Gallo P., Levi G., Peschle C., Aloisi F. The costimulatory molecule B7 is expressed on human microglia in culture and in multiple sclerosis acute lesions. J. Neuropathol. Exp. Neurol. 54:1995;175-187.
9. Jat P.S., Sharp P.A. Large T-antigens of Simian Virus-40 and polyomavirus efficiently establish primary fibroblasts. J. Virol. 59:1986;746-750.
10. Jat P.S., Cepko C.L., Mulligan R.C., Sharp P.A. Recombinant retroviruses encoding simian virus large T antigen and polyomavirus large and middle T antigens. Mol. Cell. Biol. 6:1986;1204-1217.
11. Katz-Levy Y., Neville K.L., Girvin A.M., Vanderlugt C.L., Pope J.G., Tan L.J., Miller S.D. Endogenous presentation of self myelin epitopes by CNS-resident APCs in Theiler's virus-infected mice. J. Clin. Invest. 104:1999;599-610.
12. Katz-Levy Y., Neville K.L., Padilla J., Rahbe S.M., Begolka W.S., Girvin A.M., Olson J.K., Vanderlugt C.L., Miller S.D. Temporal development of autoreactive Th1 responses and endogenous antigen presentation of self myelin epitopes by CNS-resident APCs in Theiler's virus-infected mice. J. Immunol. 165:2000;5304-5314.
13. Kreutzberg G.W. Microglia: a sensor for pathological events in the CNS. Trends Neurosci. 19:1996;312-318.
14. Kurtzke J.F. Epidemiologic evidence for multiple sclerosis as an infection. Clin. Microbiol. Rev. 6:1993;382-427.
15. Li H., Cuzner M.L., Newcombe J. Microglia-derived macrophages in early multiple sclerosis plaques. Neuropathol. Appl. Neurobiol. 22:1996;207-215.
16. Ling E.A., Ng Y.K., Wu C.H., Kaur C. Microglia: its development and role as a neuropathology sensor. Prog. Brain Res. 132:2001;61-79.
17. Lipton H.L., Friedmann A. Purification of Theiler's murine encephalomyelitis virus and analysis of the structural virion polypeptides: correlation of the polypeptide profile with virulence. J. Virol. 33:1980;1165-1172.
18. Miller S.D., Karpus W.J. The immunopathogenesis and regulation of T-cell mediated demyelinating diseases. Immunol. Today. 15:1994;356-361.
19. Miller S.D., Vanderlugt C.L., Begolka W.S., Pao W., Yauch R.L., Neville K.L., Katz-Levy Y., Carrizosa A., Kim B.S. Persistent infection with Theiler's virus leads to CNS autoimmunity via epitope spreading. Nat. Med. 3:1997;1133-1136.
20. O'Keefe G.M., Nguyen V.T., Benveniste E.N. Class II transactivator and class II MHC gene expression in microglia: modulation by the cytokines TGF-beta, IL-4, IL-13 and IL-10. Eur. J. Immunol. 29:1999;1275-1285.
21. Olson J.K., Girvin A.M., Miller S.D. Direct activation of innate and antigen presenting functions of microglia following infection with Theiler's virus. J. Virol. 75:2001;9780-9789.
22. Ota K., Matsui M., Milford E.L., Mackin G.A., Weiner H.L., Hafler D.A. T-cell recognition of an immunodominant myelin basic protein epitope in multiple sclerosis. Nature. 346:1990;183-187.
23. Righi M., Mori L., De Libero G., Sironi M., Biondi A., Mantovani A., Donini S.D., Ricciardi-Castagnoli P. Monokine production by microglial cell clones. Eur. J. Immunol. 19:1989;1443-1448.
24. Shrikant P., Benveniste E.N. The central nervous system as an immunocompetent organ: role of glial cells in antigen presentation. J. Immunol. 157:1996;1819-1822.
25. Streit W. Microglia. In: Kettermann H, Ransom BR, editors. Neuroglia. Oxford: Oxford University Press, 1995:85-96.
26. Tan L.J., Gordon K.B., Mueller J.P., Matis L.A., Miller S.D. Presentation of proteolipid protein epitopes and B7-1-dependent activation of encephalitogenic T cells by IFN-gamma-activated SJL/J astrocytes. J. Immunol. 160:1998;4271-4279.
27. Traugott U., Reinherz E.L., Raine C.S. Multiple sclerosis: distribution of T cell subsets and la-positive macrophages in lesions of different ages. J. Neuroimmunol. 4:1983;201-221.
28. Walker W.S., Gatewood J., Olivas E., Askew D., Havenith C.E. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63:1995;163-174.