Nitrite inhibition of Vitreoscilla hemoglobin (VHb) in recombinant E. coli: Direct evidence that VHb enhances recombinant protein production

Biotechnology Progress

Volumn 16, Issue 6, 2000, Pages 917-921

  Aydin, S ^a   Webster, D A ^a   Stark, B C ^a  

^a Illinois Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; BACTERIAL GENE; CELL RESPIRATION; CYTOCHROME C OXIDASE; GENETIC ENGINEERING; GENETIC STRAIN; HEMOGLOBIN; PROTEIN INHIBITOR; RECOMBINANT GENE; SODIUM NITRITE; VITREOSCILLA HEMOGLOBIN;

ALPHA-AMYLASE; ESCHERICHIA COLI; HEMOGLOBINS; NITRITES; OXYGEN; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; VITREOSCILLA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VITREOSCILLA;

EID: 0034523899     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0000757     Document Type: Article

References (41)

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3. Study of Vitreoscilla globin (vgb) gene expression and promoter activity in E. coli through transcriptional fusion
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6. The Vitreoscilla hemoglobin gene: Molecular cloning, nucleotide sequencing and genetic expression in E. coli
7. Heterologous expression of a bacterial hemoglobin improves the growth properties of recombinant Escherichia coli
8. Presence of the bacterial hemoglobin gene improves α-amylase production of a recombinant Escherichia coli strain
9. Expression of intracellular hemoglobin improves protein synthesis in oxygen-limited Escherichia coli
10. Actinorhodin production by Streptomyces coelicolor and growth of Streptomyces lividans are improved by the expression of a bacterial hemoglobin
11. The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin
12. Intracellular expression of Vitreoscilla hemoglobin alters the aerobic metabolism of Saccharomyces cerevisiae
13. Cloning and expression of the Vitreoscilla hemoglobin gene in Pseudomonas: Effects on cell growth
14. 2
15. Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of α-amylase and neutral protease in Bacillus subtilis
16. Genetic engineering of Serratia marcescens with bacterial hemoglobin gene: Effects on growth, oxygen utilization, and cell size
17. Metabolic engineering of Serratia marcescens with the bacterial hemoglobin gene: Alterations in fermentation pathways
18. Synthesis and excretion of α-amylase in vgb+ and vgb- recombinant Escherichia coli: A comparative study
19. Production of α-amylase in fed-batch cultures of vgb+ and vgb- recombinant E. coli: Some observations
20. 31P NMR and saturation transfer study
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22. Myoglobin function and energy metabolism of isolated cardiac myocytes: Effect of sodium nitrite
23. New M13 vectors for cloning
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26. Selection and characterization of α-amylase overproducing recombinant E. coli containing the bacterial hemoglobin gene
27. Spectral characteristics and interconversions of the reduced, oxidized, and oxygenated forms of purified cytochrome o
28. Variation of oxygen requirement with plasmid size in recombinant Escherichia coli
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31. Mechanism of auto-catalytic oxidation of oxyhemoglobin by nitrite: An intermediate detected by electron spin resonance
32. Reduction of nitrite to nitric oxide by enteric bacteria
33. Role for the Salmonella flavohemoglobin in protection from nitric oxide
34. Intracellular expression of Vitreoscilla hemoglobin modifies microaerobic Escherichia coli metabolism through elevated concentration and specific activity of cytochrome o
35. Nitrite and nitrate regulation at the promoters of two Escherichia coli operons encoding nitrite reductase: Identification of common target heptamers for both NarP- and NarL-dependent regulation
36. Nitric oxide, nitrite and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12
37. Regulation of the Salmonella typhimurium flavohemoglobin gene. A new pathway for bacterial gene expression in response to nitric oxide
38. Genetic engineering to contain the Vitreoscilla hemoglobin gene enhances degradation of benzoic acid by Xanthomonas maltophilia
39. Vitreoscilla hemoglobin enhances the first step in 2,4-dinitrotoluene degradation in vitro and at low aeration in vivo
40. Cloning and expression of Vitreoscilla hemoglobin gene in Burkholderia sp. strain DNT for enhancement of 2,4-dinitrotoluene degradation