The 10.8-Å structure of Saccharomyces cerevisiae phosphofructokinase determined by cryoelectron microscopy: Localization of the putative fructose 6-phosphate binding sites

Journal of Structural Biology

Volumn 143, Issue 2, 2003, Pages 124-134

  Ruiz, Teresa ^a   Mechin, Ingrid ^b   Bär, Jörg ^c   Rypniewski, Wojciech ^d,e   Kopperschläger, Gerhard ^c   Radermacher, Michael ^a  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

^d INSTITUTE OF BIOORGANIC CHEMISTRY   (Poland)

^e UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

CTF correction; Electron microscopy; Glycolytic enzyme; Phosphofructokinase; Point mode; Radon transforms; Saccharomyces cerevisiae; Simultaneous alignment; Three dimensional reconstruction; Vitreous ice; X ray

Indexed keywords

6 PHOSPHOFRUCTOKINASE; DIMER; FRUCTOSE 6 PHOSPHATE; FUNGAL ENZYME; LANTHANUM; TETRAMER; FRUCTOSE PHOSPHATE; FRUCTOSE-6-PHOSPHATE; PROTEIN SUBUNIT; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; BACTERIUM; BINDING SITE; CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPE; ENZYME ANALYSIS; ENZYME STRUCTURE; ENZYME SUBUNIT; HYDRATION; ILLUMINATION; IMAGE ANALYSIS; IMAGE RECONSTRUCTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; TECHNIQUE; X RAY ANALYSIS; YEAST; CHEMISTRY; EQUIPMENT DESIGN; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN CONFORMATION; STRUCTURAL HOMOLOGY; THREE DIMENSIONAL IMAGING;

BACTERIA (MICROORGANISMS); EUKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

BINDING SITES; CRYOELECTRON MICROSCOPY; EQUIPMENT DESIGN; FRUCTOSEPHOSPHATES; IMAGING, THREE-DIMENSIONAL; PHOSPHOFRUCTOKINASE-1; PROTEIN CONFORMATION; PROTEIN SUBUNITS; SACCHAROMYCES CEREVISIAE PROTEINS; STRUCTURAL HOMOLOGY, PROTEIN;

EID: 0041334007     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(03)00140-0     Document Type: Article

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