High-throughput production of recombinant antigens for mouse KIAA proteins in Escherichia coli: Computational allocation of possible antigenic regions, and construction of expression plasmids of glutathione-S-transferase-fused antigens by an in vitro recombination-assisted method

DNA Research

Volumn 10, Issue 3, 2003, Pages 129-136

  Hara, Yasuhiro ^a   Shimada, Kiyo ^a   Kohga, Hiroshi ^a   Ohara, Osamu ^b,c   Koga, Hisashi ^a,b  

^a Chiba Industry Advancement Center   (Japan)

^b KAZUSA DNA RESEARCH INSTITUTE   (Japan)

^c RIKEN YOKOHAMA INSTITUTE   (Japan)

Author keywords

Antibody; Antigen; High throughput; In vitro recombination assisted method; MKIAA

Indexed keywords

GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; KIAA ANTIBODY; POLYACRYLAMIDE GEL; PROTEIN; PROTEIN KIAA; RECOMBINANT ANTIBODY; RECOMBINANT ANTIGEN; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ANTIGEN PURIFICATION; ARTICLE; CLONE; DNA SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE; GENE EXPRESSION; GENE FUNCTION; GENE TRANSFER; HIGH THROUGHPUT PRODUCTION; IN VITRO STUDY; KIAA GENE; MATHEMATICAL COMPUTING; MOUSE; NONHUMAN; PLASMID; RECOMBINANT DNA TECHNOLOGY; SHOTGUN CLONE; SOLUBILITY;

ANIMALS; ANTIBODIES; ANTIGENS; CULTURE MEDIA; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; MICE; PLASMIDS; PROTEINS; RABBITS; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC;

ESCHERICHIA COLI; NEGIBACTERIA; VECTORS;

EID: 0041312395     PISSN: 13402838     EISSN: None     Source Type: Journal    
DOI: 10.1093/dnares/10.3.129     Document Type: Article

References (27)

1. Kawai, J., Shinagawa, A., Shibata, K. et al. 2001, Functional annotation of a full-length mouse cDNA collection, Nature, 409, 685-690.
2. Kargul, G. J., Dudekula, D. B., Qian, Y. et al. 2001, Verification and initial annotation of the NIA mouse 15K cDNA clone set, Nat. Genet., 28, 17-18.
3. Strausberg, R. L., Feingold, E. A., Grouse, L. et al. 2002, Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences, Proc. Natl. Acad. Sci. USA, 99, 16899-16903.
4. Nomura, N., Miyajima, N., Sazuka, T. et al. 1994, Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement), DNA Res., 1, 47-56.
5. Ohara, O., Nagase, T., Mitsui, G. et al. 2002, Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method, DNA Res., 9, 47-57.
6. Okazaki, N., Kikuno, R., Ohara, R. et al. 2002, Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries, DNA Res., 9, 179-188.
7. Okazaki, N., Kikuno, R., Ohara, R. et al. 2003, Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries, DNA Res., 10, 35-48.
8. Waterston, R. H., Lindblad-Toh, K., Birney, E. et al. 2002, Initial sequencing and comparative analysis of the mouse genome, Nature, 420, 520-562.
9. Nolan, P. M., Peters, J., Strivens, M. et al. 2000, A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse, Nat. Genet., 25, 440-443.
10. Hrabe de Angelis, M., and Strivens, M. 2001, Large-scale production of mouse phenotypes: the search for animal models for inherited diseases in humans, Brief Bioinform, 2, 170-180.
11. Boyd, Y., Blair, H. J., Cunliffe, P., Masson, W. K., and Reed, V. 2000, A phenotype map of the mouse X chromosome: models for human X-linked disease, Genome Res., 10, 277-292.
12. Chou, P. Y. and Fasman, G. D. 1978, Empirical predictions of protein conformation, Annu. Rev. Biochem., 47, 251-276.
13. Garnier, J., Osguthorpe, D. J., and Robson, B. 1978, Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins, J. Mol. Biol., 120, 97-120.
14. Hopp, T. P. and Woods, K. R. 1981, Prediction of protein antigenic determinants from amino acid sequences, Proc. Natl. Acad. Sci. USA, 78, 3824-3828.
15. Emini, E. A., Hughes, J. V., Perlow, D. S., and Boger, J. 1985, Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide, J. Virol., 55, 836-839.
16. Karplus, P. A. and Schulz, G. E. 1985, Prediction of chain flexibility in proteins: a tool for the selection of peptide antigen, Naturwissenschaften, 72, 212-213.
17. Jameson, B. A. and Wolf, H. 1988, The antigenic index: a novel algorithm for predicting antigenic determinants, Comput. Appl. Biosci., 4, 181-186.
18. Harlow, E. D. and Lane, D. 1988, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
19. Hirokawa, T., Boon-Chieng, S., and Mitaku, S. 1998, SOSUI: classification and secondary structure prediction system for membrane proteins, Bioinformatics, 14, 378-379.
20. Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989, Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
21. Smith, D. B. and Johnson, K. S. 1988, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase, Gene, 67, 31-40.
22. Coligan, J. E., Dunn, B. M., Speicher, D. W., and Wingfield, P. J., 2002, Current Protocols in Protein, John Wiley & Sons, Inc. New Jersey, pp. 5.17.1-5.17.10.
23. Braun, P., Hu, Y., Shen, B. et al. 2002, Proteome-scale purification of human proteins from bacteria, Proc. Natl. Acad. Sci. USA, 99, 2654-2659.
24. Murray, A. M., Kelly, C. D., Nussey, S. S., and Johnstone, A. P. 1998, Production of glutathione-coated microtitre plates for capturing recombinant glutathione S-transferase fusion proteins as antigens in immunoassays, J. Immunol. Methods, 218, 133-139.
25. Rabin, D. U., Palmer-Crocker, R., Mierz, D. V., and Yeung, K. K. 1992, An ELISA sandwich capture assay for recombinant fusion proteins containing glutathione-S-transferase, J. Immunol. Methods, 156, 101-105.
26. Sehr, P., Zumbach, K., and Pawlita, M. 2001, A generic capture ELISA for recombinant proteins fused to glutathione S-transferase: validation for HPV serology, J. Immunol. Methods, 253, 153-162.
27. Coligan, J. E., Dunn, B. M., Speicher, D. W., and Wingfield, P. J. 1997, Current Protocols in Protein, John Wiley & Sons, Inc. New Jersey, pp. 6.6.1-6.6.21.