Gas chromatographic method for determining very long chain fatty acids that compose D003 in aqueous suspensions from 20 to 200 mg/ml, used in pharmacological and toxicological studies

Farmaco

Volumn 58, Issue 7, 2003, Pages 521-525

  Díaz, Yanet Tejeda ^a   Méndez Antolín, Ernesto ^a   Sierra Pérez, Roxana ^a   Marrero Delange, David ^a   Canavaciolo, Víctor González ^a   Laguna Granja, Abilio ^a  

^a NATL CENTER FOR SCIENTIFIC RESEARCH   (Cuba)

Author keywords

D003; Fatty acids; GC determination; Suspensions; Validation

Indexed keywords

CHLOROFORM; D 003; DECANOIC ACID DERIVATIVE; HYDROCHLORIC ACID; LONG CHAIN FATTY ACID; METHANOL;

ACCURACY; AQUEOUS SOLUTION; ARTICLE; CORRELATION COEFFICIENT; DRUG EFFECT; EXTRACTION; GAS CHROMATOGRAPHY; STANDARD; STUDENT T TEST; TECHNIQUE; VALIDATION PROCESS;

EID: 0038798611     PISSN: 0014827X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-827X(03)00072-7     Document Type: Article

References (13)

1. L. González, D. Marrero, A. Laguna, R. Más, M.L. Arruzazabala, D. Carbajal, M. Cora, R. Menéndez (Laboratorios DALMER S.A.), Mixture of primary fatty acids obtained from sugar cane wax, Patent CU 22,723; PCT Application WO 98/43631, 1997.
2. E. Uribarri, M. González, A. Laguna, D. Marrero, Physicochemical characterization of D003, A mixture of primary fatty acids isolated from sugar cane wax with antiplatelet and cholesterol-lowering effects, Eur. J. Pharm. Biopharm. (2002) in press.
3. Gámez R., Mendoza S., Más R., Mesa R., Castaño G., Marrero D. Dose-dependent cholesterol-lowering effects of D003 on normocholesterolemic rabbits. Curr. Ther. Res. 61:2000;460-468.
4. Mendoza S., Gámez R., Noa M., Más R., Castaño G., Mesa R., Mesa M., de Armas M. The effects of D-003 and policosanol on the lipid profile and endothelemia cells in normocholesterolemic rabbits: a head to head comparison. Curr. Ther. Res. 62:2001;209-220.
5. Menéndez R., Más R., Marrero D., Amor A., Rodeiro I. Inhibition of cholesterol biosynthesis in cultured fibroblasts by D003, a mixture of very long-chain saturated fatty acids. Atherosclerosis. 151:2000;131. (special issue).
6. Molina V., Arruzazabala M.L., Carbajal D., Más R., Valdés S. Antiplatelet and antitrombotic effect of D003. Pharmacol. Res. 42:2000;137-143.
7. Gámez R., Más R., Noa M., Menéndez R., Alemán C., Acosta C.P., García H., Hernández C., Amor A.M., Pérez J., Goicochea E. Acute and oral subcrhonic toxicity of D003 in rats. Toxicol. Lett. 118:2000;31-41.
8. Gámez R., González J.E., Rodeiro I., Fernández I., Alemán C., Rodríguez M.D., Acosta P.C., García H. In vivo genotoxic evaluation of D003: a mixture of very long-chain saturated fatty acids. J. Med. Food. 4:2001;85-91.
9. Christie W.W. Gas chromatography-mass spectrometry methods for structural analysis of fatty acids. Lipids. 33:1998;343-353.
10. Christie W.W. Gas Chromatography and Lipids, A Practical Guide. 1989;64-84 The Oily Press, Ayr, Scotland.
11. Novák J. Quantitative Analysis by Gas Chromatography. 2nd ed. 1988;79-135 Marcel Dekker, New York.
12. M. Castro, S. Gascón, M. Pujol, J. Sans, L. Vicente, Validation of Analytical Methods, Spanish Association of the Industry Pharmacists, Catalan Section, Edited under HP license, 1989, pp. 31-50.
13. Christie W.W. Gas Chromatography and Lipids, A Practical Guide. 1989;125 The Oily Press, Ayr, Scotland.