Flow cytometric analysis of macaque whole blood for antigen-specific intracellular cytokine production by T lymphocytes

Journal of Medical Primatology

Volumn 32, Issue 1, 2003, Pages 23-30

  Keeney, Talitha S ^a   Nomura, Laurel E ^b   Maecker, Holder T ^b   Jagannadha Sastry, K ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^b BD BIOSCIENCES   (United States)

Author keywords

CD4; CD8; Flow cytometry; IFN ; IL 2; Macaca mulatta; Non human primate; Rhesus macaque; SEB; TNF

Indexed keywords

ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; GAMMA INTERFERON; GLYCOPROTEIN GP 160; HUMAN IMMUNODEFICIENCY VIRUS VACCINE; RECOMBINANT PROTEIN; RHESUS ANTIGEN; STAPHYLOCOCCUS ENTEROTOXIN; TUMOR NECROSIS FACTOR ALPHA; VIRUS PROTEIN;

ANIMAL EXPERIMENT; ANTIGEN SPECIFICITY; ARTICLE; BLOOD ANALYSIS; BLOOD SAMPLING; CONTROLLED STUDY; CYTOKINE PRODUCTION; FLOW CYTOMETRY; HUMAN IMMUNODEFICIENCY VIRUS; IMMUNE RESPONSE; IMMUNOSTIMULATION; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; RHESUS IMMUNIZATION; RHESUS MONKEY; STANDARDIZATION; T LYMPHOCYTE;

ANIMALS; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; ENTEROTOXINS; FLOW CYTOMETRY; HIV ENVELOPE PROTEIN GP160; INTERFERON TYPE II; MACACA MULATTA; RECOMBINANT PROTEINS; T-LYMPHOCYTES; TUMOR NECROSIS FACTOR-ALPHA;

ALPHAVIRUS; ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS; MACACA; MACACA MULATTA; PRIMATES; STAPHYLOCOCCUS;

EID: 0038782975     PISSN: 00472565     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0684.2003.00005.x     Document Type: Article

References (18)

1. BORROW P, LEWICKI H, HAHN BH, SHAW GM, OLDSTONE MBA: Virus specific CD8 cytotoxic T-lymphocyte activity associated with control of viremia in primary human immunodeficiency virus type 1 infection. J Virol 68:6103-6110, 1994.
2. DYKHUIZEN M, CEMAN J, MITCHEN J, ZAYAS M, MACDOUGALL A, HELGELAND J, RAKASZ E, PAUZA D: Importance of the CD3 marker for evaluating changes in Rhesus macaque CD4/CD8 T-cell ratios. Cytometry 40:69-75, 2000.
3. FIEBIG EW, JOHNSON DK, HIRSCHKORN DF, KNAPE CC, WEBSTER HK, LOWDER J, BUSCH MP: Lymphocyte subset analysis on frozen whole blood. Cytometry 29:340-350, 1997.
4. GAINES H, ANDERSSON L, BIBERFELD G: A new method for measuring lymphoproliferation at the single-cell level in whole blood cultures by flow cytometry. J Immunol Methods 195:63-72, 1996.
5. HERZENBERG LA, DE ROSA SC, HERZENBERG LA: Monoclonal antibodies and the FACS: complementary tools for immunobiology and medicine. Immunol Today 21:383-390, 2000.
6. HODGE G, HODGE S, HAN P: Increased levels of apoptosis of leukocyte subsets in cultured PBMCs compared to whole blood as shown by annexin V binding: relevance to cytokine production. Cytokine 12:1763-1768, 2000.
7. JUNG T, SCHAUER U, HEUSSER C, NEUMANN C, RIEGER C: Detection of intracellular cytokines by flow cytometry. J Immunol Methods 159:197-207, 1993.
8. KOUP RA, SAFRIT JT, CAO Y, ANDREWS CA, MCLEOD G, BORKOWSKY W, FARTHING C, HO DD: Temporal associations of cellular immune responses with the initial control of viremia in primary human immunodeficiency virus type 1 syndrome. J Virol 68:4650-4655, 1994.
9. MAINO VC, PICKER LJ: Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression. Cytometry 34:207-215, 1998.
10. NEHETE PN, SATTERFIELD WC, MATHERNE CM, ARLINGHAUS RB, SASTRY KJ: Induction of Human Immunodeficiency Virus-specific T cell responses in rhesus monkeys by synthetic peptides from gp160. AIDS Res and Human Retroviruses 9(3):235-240, 1993.
11. NEHETE PN, CHITTA S, HOSSAIN MM, HILL L, BERNACKY BJ, BAZE W, ARLINGHAUS RB, SASTRY KJ: Protection against chronic infection and AIDS by an HIV envelope peptide-cocktail vaccine in a pathogenic SHIV-rhesus model. Vaccine 20:813-825, 2001.
12. NILSSON C. MAKITALO B, THORSTENSSON R, NORLEY S, BINNINGER-SCHINZEL D, CRANAGE M, RUD E, BIBERFELD G, PUTKONEN P: Live attenuated simian immunodeficiency virus (SIV)mac in macaques can induce protection against mucosal infection with SIVsm. AIDS 12:2261-2270, 1998.
13. + T cells. Cytometry 40:60-68, 2000.
14. OGG GS, JIN X, BONHOEFFER S, DUNBAR PR, NOWAK MA, MONARD S, SEGAL JP, CAO Y, ROWLAND-JONES SL, CERUNDOLO V, HURLEY A, MARKOWITZ M, HO DD, NIXON DF, MCMICHAEL AJ: Quantitation of HIV-1-specific cytotoxic T lymphocytes and plasma load of viral RNA. Science 279:2103-2106, 1998.
15. RINALDO C, HUANG XL, FAN ZF, DING M, BELTZ L, LOGAR A, PANICALI D, MAZZARA G, LIEBMANN J, COTTRILL M, GUPTA P: High levels of anti-human immunodeficiency virus type 1 (HIV-1) memory cytotoxic T lymphocyte activity and low viral load are associated with lack of disease in HIV-1 infected long-term nonprogressors. J Virol 69:5838-5842, 1995.
16. ROBINSON HL, MONTEFIORI DC, JOHNSON RP, MANSON KH, KALISH ML, LIFSON JD, RIZVI TA, LU S, HU SL, MAZZARA GP, PANICALI DL, HERNDON JG, GLICKMAN R, CANDIDO MA, LYDY SL, WYAND MS, MCCLURE HM: Neutralizing antibody-independent containment of immunodeficiency virus challenge by DNA priming and recombinant pox virus boost immunizations. Nat Med 5:526-534, 1999.
17. SCHMITZ JE, KURODA MJ, SANTRA S, SASSEVILLE VG, SIMON MA, LIFTON MA, RACZ P, TENNER-RACZ K, DALESANDRO M, SCALLON BJ, GHRAYEB J, FORMAN MA, MONTEFIORI DC, RIEBER EP, LETVIN NL, REIMANN KA: Control of viremia in simian immunodeficiency syndrome virus replication in peripheral blood lymphocytes. Science 283:857-860, 1999.
18. SUNI MA, PICKER LJ, MAINO VC: Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J Immunol Methods 212: 89-98, 1998.