Characterization of an ascorbate peroxidase in plastids of tobacco BY-2 cells

Physiologia Plantarum

Volumn 117, Issue 4, 2003, Pages 550-557

  Madhusudhan, Rapolu ^a,b   Ishikawa, Takahiro ^a,b   Sawa, Yoshihiro ^a,b   Shigeoka, Shigeru ^c   Shibata, Hitoshi ^a,b  

^a SHIMANE UNIVERSITY   (Japan)

^b TOTTORI UNIVERSITY   (Japan)

^c KINKI UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ENZYMES; FLUORESCENCE; MOLECULAR WEIGHT;

PLASTIDS;

TOBACCO;

AMINO ACIDS; ENZYMES; FLUORESCENCE; MOLECULAR WEIGHT; TOBACCO;

EMBRYOPHYTA; NICOTIANA TABACUM; SPINACIA OLERACEA;

EID: 0038734441     PISSN: 00319317     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3054.2003.00066.x     Document Type: Article

References (33)

1. Amako K, Chen GX, Asada K (1994) Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant Cell Physiol 35: 497-504
2. 2 scavenging in plants. In: Scandalios JG (ed) Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Cold Spring Harbor Laboratory Press, New York, pp. 715-735
3. Burgess N, Beakes GW, Thomas DR (1985) Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons. Planta 166: 151-155
4. Chen GX, Asada K (1989) Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol 30: 987-998
5. Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Curr Biol 6: 325-330
6. Dalton DA, Baird LM, Langeberg L, Taugher CY, Anyan WR, vance CP, Sarath G (1993) Subcellular localization of oxygen defense enzymes in soybean (Glycine max [L.] Merr.) root nodules. Plant Physiol 102: 481-489
7. De Leonardis S, Dipierro N, Dipierro S (2000) Purification and characterization of an ascorbate peroxidase from potato tuber mitochondria. Plant Physiol Biochem 38: 773-779
8. Gross P, ap Rees T (1986) Alkaline inorganic pyrophosphatase and starch synthesis in amyloplasts. Planta 167: 140-145
9. Ishikawa T, Takeda T, Kohno H, Shigeoka S (1996a) Molecular characterization of Euglena ascorbate peroxidase using monoclonal antibody. Biochim Biophys Acta 1290: 69-75
10. Ishikawa T, Takeda T, Shigeoka S (1996b) Purification and characterization of cytosolic ascorbate peroxidase from Komatsuna (Brassica rapa). Plant Sci 120: 11-18
11. Ishikawa T, Yoshimura K, Sakai K, Tamoi M, Takeda T, Shigeoka S (1998) Molecular characterization and physiological role of a glyoxysome-bound ascorbate peroxidase form spinach. Plant Cell Physiol 39: 23-34
12. Ishikawa T, Yoshimura K, Tamoi M, Takeda T, Shigeoka S (1997) Alternative mRNA splicing of 3′-terminal exons generates ascorbate peroxidase isoenzymes in spinach chloroplasts. Biochem J 328: 795-800
13. Iturbe-Ormaetxe I, Matamoros MA, Rubio MC, Dalton DA, Becana M (2001) The antioxidants of legume nodule mitochondria. Mol Plant Microbe Interact 14: 1189-1196
14. Jiménez A, Hernández JA, del Río LA, Sevilla F (1997) Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves. Plant Physiol 114: 275-284
15. Kruft V, Eubel H, Jänsch L, Werhahn W, Braun HP (2001) Proteomic approach to identify novel mitochondrial proteins in Arabidopsis. Plant Physiol 127: 1694-1710
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
17. Millar AH, Sweetlove LJ, Giegé P, Leaver CJ (2001) Analysis of the Arabidopsis mitochondrial proteome. Plant Physiol 127: 1711-1727
18. Mittova V, Guy M, Tal M, Volokita M (2002) Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: increased activities of antioxidant enzymes in root plastids. Free Radic Res 36: 195-202
19. Miyake C, Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol 33: 541-553
20. Miyake C, Cao WH, Asada K (1993) Purification and molecular properties of the thylakoid-bound ascorbate peroxidase in spinach chloroplasts. Plant Cell Physiol 34: 881-889
21. Moran JF, Iturbe-Ormaetxe I, Matamoros MA, Rubio MC, Clemente MR, Brewin NJ, Becana M (2000) Glutathione and homoglutathione synthetases of legume nodules. Cloning, expression, and subcellular localization. Plant Physiol 124: 1381-1392
22. Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the 'HeLa' cell in the the cell biology of higher plants. Int Rev Cytol 132: 1-30
23. Nakano Y, Asada K (1987) Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate depleted medium. Plant Cell Physiol 28: 131-140
24. Peeters N, Small I (2001) Dual targeting to mitochondria and chloroplasts. Biochim Biophys Acta 1541: 54-63
25. Prasad TK, Anderson MD, Stewart CR (1995) Localization and characterization of peroxidases in the mitochondria of chilling-acclimated maize seedlings. Plant Physiol 108: 1597-1605
26. Rubio MC, Ramos J, Webb KJ, Minchin FR, Gonzalez E, Arrese-Igor C, Becana M (2001) Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, posttranslational regulation, and compensation of isozyme activities. Mol Plant Microbe Interact 14: 1178-1188
27. Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53: 1305-1319
28. Tang X, Webb MA (1994) Soybean root nodule cDNA encoding glutathione reductase. Plant Physiol 104: 1081-1082
29. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76: 4350-4354
30. Wada K, Tada T, Nakamura Y, Yabuta Y, Yoshimura K, Takeda T, Shigeoka S, Nishimura K (2002) Crystallization and preliminary X-ray diffraction analysis of chloroplastic ascorbate peroxidase of tobacco plants. Acta Cryst 58: 559-561
31. Watanabe N, Che FS, Iwano M, Takayama S, Yoshida S, Isogai A (2001) Dual targeting of spinach protoporphyrinogen oxidase II to mitochondria and chloroplasts by alternative use of two in-frame initiation codons. J Biol Chem 276: 20474-20481
32. Yoshimura K, Yabuta Y, Ishikawa T, Shigeoka S (2002) Identification of a cis-element for tissue-specific alternative splicing of chloroplast ascorbate peroxidase pre-mRNA in higher plants. J Biol Chem 277: 40623-40632
33. Yoshimura K, Yabuta Y, Tamoi M, Ishikawa T, Shigeoka S (1999) Alternatively spliced mRNA variants of chloroplast ascorbate peroxidase isoenzymes in spinach leaves. Biochem J 338: 41-48