Purification and characterization of cytosolic ascorbate peroxidase from komatsuna (Brassica rapa)

Plant Science

Volumn 120, Issue 1, 1996, Pages 11-18

  Ishikawa, Takahiro ^a   Takeda, Toru ^a   Shigeoka, Shigeru ^a  

^a KINKI UNIVERSITY   (Japan)

Author keywords

Ascorbate peroxidase; Cytosol; Komatsuna (Brassica rapa); Purification

Indexed keywords

ASCORBIC ACID; PEROXIDASE;

ARTICLE; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME PURIFICATION; NONHUMAN; PHOTOSYNTHESIS; PLANT; PLANT LEAF;

EID: 0030592380     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(96)04472-X     Document Type: Article

References (24)

1. [1] K. Asada, Production and action of active oxygen species in photosynthetic tissues, in: C.H. Foyer and P.M. Mullineaux (Eds.), Causes of photooxidative stress and amelioration of defense systems in plants, CRC Press, Boca Raton, 1994, pp. 77-104.
2. [2] K. Yamaguchi, H. Mori and M. Nishimura, A novel isoenzyme of ascorbate peroxidase localized on glyoxysomal and leaf peroxisomal membranes in pumpkin. Plant Cell Physiol., 36 (1995) 1157-1162.
3. [3] J.R. Bunkelmann and R.N. Trelease, Ascorbate peroxidase: A prominent membrane protein in oilseed glyoxysomes. Plant Physiol., 110 (1996) 589-598.
4. 2 fixation of isolated chloroplasts. Biochim. Biophys. Acta, 440 (1976) 476-482.
5. [5] R. Mittler and B.A. Zilinskas, Purification and characterization of pea cytosolic ascorbate peroxidase. Plant Physiol., 97 (1991) 962-968.
6. [6] M.-L. Schantz, H. Schreiber, P. Guillemaut, and R. Schantz, Change in ascorbate peroxidase activities during fruit ripening in Capsicum annuum. FEBS Lett., 358 (1995) 149-152.
7. [7] T. Ishikawa, K. Sakai, T. Takeda and S. Shigeoka, Cloning and expression of cDNA encoding a new type of ascorbate peroxidase from spinach. FEBS Lett., 367 (1995) 28-32.
8. [8] S. Shigeoka, Y. Nakano and S. Kitaoka, Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase. Biochem. J., 186 (1980) 377-380.
9. [9] D.A. Dalton, F.J. Hanus, S.A. Russell and H.J. Evans, Purification, properties and distribution of ascorbate peroxidase in legume root nodules. Plant Physiol., 83 (1987) 789-794.
10. [10] M.R. Elia, G. Borraccino and S. Dipierro, Soluble ascorbate peroxidase from potato tubers. Plant Sci., 85 (1992) 17-21.
11. [11] K. Amako and K. Asada, Separate assays of ascorbate peroxidase and guaiacol peroxidase and of chloroplastic and cytosolic isozymes of ascorbate peroxidase, in: M. Murata (Ed.), Research in photosynthesis, 4. Kluwer, Dordrecht, 1992, pp. 529-532.
12. [12] U.K. Leammli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 (1970) 680-685.
13. [13] H. Towbin, T. Stahelin and J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA, 76 (1979) 4350-4354.
14. [14] T. Ishikawa, T. Takada, H. Kohno and S. Shigeoka, Molecular characterization of Euglena ascorbate peroxidase using monoclonal antibody. Biochim. Biophys. Acta, 1290 (1996) 69-75.
15. [15] O.H. Lowry, N.J. Rosebrough, A.L. Farr and R.J. Randall, Protein measurement with Folin phenol reagent. J. Biol. Chem., 193 (1951) 265-275.
16. [16] K. Amako, G.-X. Chen and K. Asada, Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant Cell Physiol., 35 (1994) 497-504.
17. [17] G.-X. Chen and K. Asada, Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol., 30 (1989) 987-998.
18. [18] K. Tanaka, E. Takeuchi, A. Kubo, T. Sakaki, K. Haraguchi and Y. Kawamura, Two immunologically different isozymes of ascorbate peroxidase from spinach leaves. Arch. Biochem. Biophys., 286 (1991) 371-375.
19. [19] C. Miyake, W.-H. Cao and K. Asada, Purification and molecular properties of thylakoid-bound ascorbate peroxidase in spinach chloroplasts. Plant Cell Physiol., 34 (1993) 881-889.
20. [20] S. Shigeoka, Y. Nakano and S. Kitaoka, Purification and some properties of L-ascorbic acid-specific peroxidase in Euglena gracilis Z. Arch. Biochem. Biophys., 201 (1980) 121-127.
21. [21] R. Mittler and B.A. Zilinskas, Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase. FEBS Lett., 289 (1991) 257-259.
22. [22] A. Kubo, H. Saji, K. Tanaka and N. Kondo, Cloning and sequencing of a cDNA encoding ascorbate peroxidase from Arabidopsis thaliana. Plant Mol. Biol., 18 (1992) 691-701.
23. [23] R. Mittler and B.A. Zilinskas, Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Plant J., 5 (1994) 397-405.
24. [24] C.H. Foyer, M. Lelandais and K.J. Kunert, Photooxidative stress in plants. Physiol. Plant., 92 (1994) 696-717.