Actinomycin D inhibiting K562 cell apoptosis elicited by salvicine but not decreasing its cytotoxicity

Acta Pharmacologica Sinica

Volumn 24, Issue 5, 2003, Pages 415-421+478

  Qing, Chen ^a,c   Miao, Ze Hong ^a   Tong, Lin Jiang ^a   Zhang, Jin Sheng ^b   Ding, Jian ^a  

^a Division of Antitumor Pharmacology   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c Yunnan Pharmacological Laboratories of Natural Products   (China)

Author keywords

Actinomycin D; Apoptosis; Cytotoxicity; K562 cells; Salvicine

Indexed keywords

DACTINOMYCIN; DITERPENOID; SALVICINE; TETRAZOLIUM; UNCLASSIFIED DRUG;

AGAR GEL ELECTROPHORESIS; APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL GROWTH; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GROWTH INHIBITION; HUMAN; HUMAN CELL;

ANTIBIOTICS, ANTINEOPLASTIC; ANTINEOPLASTIC AGENTS, PHYTOGENIC; APOPTOSIS; DACTINOMYCIN; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG INTERACTIONS; DRUG SCREENING ASSAYS, ANTITUMOR; HUMANS; K562 CELLS; NAPHTHOQUINONES;

EID: 0038708466     PISSN: 16714083     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Zhang JS, Ding J, Tang QM, Li M, Zhao M, Lu LJ, et al. Synthesis and antitumor activity of novel diterpenequinone salvicine and the analogs. Bioorg Med Chem Lett 1999; 9: 2731-6.
2. Qing C, Zhang JS, Ding J. In vitro cytotoxicity of salvicine, a novel diterpenoid quinone. Acta Pharmacol Sin 1999; 20: 297-302.
3. Meng LH, He XH, Zhang JS, Ding J. DNA topoisomerase II as primary cellular target for salvicine in Saccharomyces cerevisiae. Acta Pharmacol Sin 2001; 21: 741-6.
4. Meng LH, Zhang JS, Ding J. Salvicine, a novel DNA topoisomerase II inhibitor, exerting its effect by trapping the enzyme-DNA cleavage complex. Biochem Pharmacol 2001; 62: 733-41.
5. Tang T, Miao ZH, Ding J. Salvicine circumventing multidrug resistance and inducing multidrug resistant K562/A02 cell apoptosis. In: Xu B, Xu JH, editors. Progress in anticancer drugs and tumor chemotherapy. Beijing: Science Press; 2001. p193-203.
6. Hengartner MO. The biochemistry of apoptosis. Nature 2000; 407: 770-6.
7. Takeda M, Kobayashi M, Shirato I, Endou H. Involvement of macromolecule synthesis, endonuclease activation and c-fos expression in cisplatin-induced apoptosis of mouse proximal tubule cells. Toxicol Lett 1998; 94: 83-92.
8. Shang X, Shiono Y, Fujita Y, Oka S, Yamazaki Y. Synergistic enhancement of apoptosis by DNA- and cytoskeleton-damaging agents: a basis for combination chemotherapy of cancer. Anticancer Res 2001; 21: 2585-9.
9. Zhang B, Gojo I, Fenton RG. Myoloid cell factor-1 is a critical survival factor for multiple myeloma. Blood 2002; 99: 1885-93.
10. Chung YH, Youn J, Choi Y, Paik DJ, Cho YJ. Requirement of de novo protein synthesis for aminopterin-induced apoptosis in a mouse myeloma cell line. Immunol Lett 2001; 77: 127- 31.
11. 2-induced apoptosis in breast cancer cells. J Biol Chem 2001; 276: 47131-5.
12. Griffith TS, Fialkov JM, Scott DL, Azuhata T, Williams RD, Wall NR, et al. Induction and regulation of tumor necrosis factor-related apoptosis-inducing ligand/Apo-2 ligand -mediated apoptosis in renal cell carcinoma. Cancer Res 2002; 62: 3093-9.
13. Glazyrin AL, Chinni S, Alhasan S, Adsay VN, Vaitkevicius VK, Sarkar FH. Molecular mechanism(s) of actinomycin-D induced sensitization of pancreatic cancer cells to CD95 mediated apoptosis. Int J Oncol 2002; 20: 201-5.
14. Qing C, Jiang C, Zhang JS, Ding J. Induction of apoptosis in human leukemia K-562 and gastric carcinoma SGC-7901 cells by salvicine, a novel anticancer compound. Anti Cancer Drugs 2001; 12: 51-6.
15. Meng LH, Ding J. Induction of bulk and c-myc P2 promoter-specific DNA damage by an anti-topoisomerase II agent salvicine is an early event leading to apoptosis in HL-60 cells. FEBS Lett 2001; 501: 59-64.
16. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 1988; 48: 589-601.
17. Mosmman T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Meth 1983; 65: 55-63.
18. Zhou JJ, Yue XF, Han JX, Yang WY. Improved MTT assay for activity of antitumor agents. Chin J Pharm 1993; 24: 455-7
19. Slin N, Stafford DW. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res 1976; 3: 2302
20. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, et al. Features of apoptotic cells measured by flow cytometry. Cytometry 1992; 13: 795-801
21. Sobell HM, Jain SC. Stereochemistry of actinomycin D binding to DNA: II. Detailed molecular model of actinomycin-DNA complex and its implications. J Mol Biol 1972; 68: 21-34.
22. Liu X, Chen H, Patel DJ. Solution structure of actinomycin-DNA complexes: drug interaction at isolated G-C sites. J Biomol NMR 1991; 1: 323-47.
23. Yu C, Tseng YY. NMR study of the solution confirmation of actinomycin D. Eur J Biochem 1992; 209: 181-7.
24. Ross WE, Bradley MO. DNA double-strand breaks in mammalian cells after exposure to intercalating agents. Biochem Biophys Acta 1981; 654: 129-34.