Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood

Methods in Molecular Biology

Volumn 215, Issue , 2003, Pages 427-436

  Kalinski, Pawel ^a   Vieira, Pedro ^b   Schuitemaker, Joost H N ^b   Cai, Quan ^a   Kapsenberg, Martien ^b  

^a UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

CD1a Expression; Final Conc; Peripheral Blood Monocyte; Phorbol Myristate Acetate; Staphylococcus Aureus Cowan

Indexed keywords

CD45 ANTIGEN; CYTOKINE;

ARTICLE; CD4+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL SEPARATION; CULTURE TECHNIQUE; DENDRITIC CELL; HUMAN; METABOLISM; METHODOLOGY; MONOCYTE; PHYSIOLOGY;

ANTIGENS, CD45; CD4-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SEPARATION; CYTOKINES; DENDRITIC CELLS; HUMANS; MONOCYTES;

EID: 0038687281     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-345-3:427     Document Type: Chapter

References (25)

1. Banchereau, J. and Steinman, R. M. (1998) Dendritic cells and the control of immunity. Nature 392, 245–252.
2. Bhardwaj, N. (1997) Interactions of viruses with dendritic cells: a double-edged sword. J. Exp. Med. 186, 795–799.
3. Kalinski, P., Hilkens, C. M., Wierenga, E. A., and Kapsenberg, M. L. (1999) T-cell priming by type-1 and type-2 polarized dendritic cells: the concept of a third signal. Immunol. Today 20, 561–567.
4. Moser, M. and Murphy, K. M. (2000) Dendritic cell regulation of TH1–TH2 development. Nat. Immunol. 1, 199–205.
5. Pulendran, B., Palucka, K., and Banchereau, J. (2001) Sensing pathogens and tuning immune responses. Science 293, 253–256.
6. Liu, Y. J., Kanzler, H., Soumelis, V., and Gilliet, M. (2001) Dendritic cell lineage, plasticity and cross-regulation. Nat. Immunol. 2, 585–589.
7. Kalinski, P., Hilkens, C. M., Snijders, A., Snijdewint, F. G., and Kapsenberg, M. L. (1997) IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T-helper cells. J. Immunol. 159, 28–35.
8. De Smedt, T., Van Mechelen, M., De Becker, G., Urbain, J., Leo, O., and Moser, M. (1997) Effect of interleukin-10 on dendritic cell maturation and function. Eur. J. Immunol. 27, 1229–1235.
9. McRae, B. L., Semnani, R. T., Hayes, M. P., and van Seventer, G. A. (1998) Type I IFNs inhibit human dendritic cell IL-12 production and Th1 cell development. J. Immunol. 160, 4298–4304.
10. Kalinski, P., Schuitemaker, J. H., Hilkens, C. M., and Kapsenberg, M. L. (1998) Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+ CD83+ dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation. J. Immunol. 161, 2804–2809.
11. Vieira, P. L., Kalinski, P., Wierenga, E. A., Kapsenberg, M. L., and de Jong, E. C. (1998) Glucocorticoids inhibit bioactive IL-12p70 production by in vitro-generated human dendritic cells without affecting their T cell stimulatory potential. J. Immunol. 161, 5245–5251.
12. de Jong, E. C., Vieira, P. L., Kalinski, P., and Kapsenberg, M. L. (1999) Cortico-steroids inhibit the production of inflammatory mediators in immature monocyte-derived DC and induce the development of tolerogenic DC3. J. Leukoc. Biol. 66, 201–204.
13. Vanderheyde, N., Verhasselt, V., Goldman, M., and Willems, F. (1999) Inhibition of human dendritic cell functions by methylprednisolone. Transplantation 67, 1342–1347
14. Vieira, P. L., de Jong, E. C., Wierenga, E. A., Kapsenberg, M. L., and Kalinski, P. (2000) Development of Th1-inducing capacity in myeloid dendritic cells requires environmental instruction. J. Immunol. 164, 4507–4512.
15. Gagliardi, M. C., Sallusto, F., Marinaro, M., Langenkamp, A., Lanzavecchia, A., and De Magistris, M. T. (2000) Cholera toxin induces maturation of human dendritic cells and licences them for Th2 priming. Eur. J. Immunol. 30, 2394–2403.
16. Kalinski, P., Schuitemaker, J. H., Hilkens, C. M., Wierenga, E. A., and Kapsenberg, M. L. (1999) Final maturation of dendritic cells is associated with impaired responsiveness to IFN-gamma and to bacterial IL-12 inducers: decreased ability of mature dendritic cells to produce IL-12 during the interaction with Th cells. J. Immunol. 162, 3231–3236.
17. Langenkamp, A., Messi, M., Lanzavecchia, A., and Sallusto, F. (2001) Kinetics of dendritic cell activation: impact on priming of Th1, Th2 and nonpolarized T cells. Nat. Immunol. 1, 311–316.
18. Kalinski, P, Smits, H. H., Schuitemaker, J. H., Vieira, P. L., van Eijk, M., de Jong, E. C., et al. (2000) IL-4 is a mediator of IL-12p70 induction by human Th2 cells: reversal of polarized Th2 phenotype by dendritic cells. J. Immunol. 165, 1877–1881.
19. Peters, J. H., Xu, H., Ruppert, J., Ostermeier, D., Friedrichs, D., and Gieseler, R. K. (1993) Signals required for differentiating dendritic cells from human monocytes in vitro. Adv. Exp. Med. Biol. 329, 275–280.
20. Sallusto, F. and Lanzavecchia, A. (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J. Exp. Med. 179, 1109–1118.
21. Jonuleit, H., Kuhn, U., Muller, G., Steinbrink, K., Paragnik, L., Schmitt, E., et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27, 3135–3142.
22. Fraser, J. D. (1989) High-affinity binding of staphylococcal enterotoxins A and B to HLA-DR. Nature 339, 221–223.
23. Hilkens, C. M., Kalinski, P., de Boer, M., and Kapsenberg, M. L. (1997) Human dendritic cells require exogenous interleukin-12-inducing factors to direct the development of naive T-helper cells toward the Th1 phenotype. Blood 90, 1920–1926.
24. Snijders, A., Kalinski, P., Hilkens, C. M., and Kapsenberg, M. L. (1998) High-level IL-12 production by human dendritic cells requires two signals. Int. Immunol. 10, 1593–1598.
25. Kalinski, P., Vieira, P. L., Schuitemaker, J. H., de Jong, E. C., and Kapsenberg, M. L. (2001) Prostaglandin E2 is a selective inducer of IL-12p40 production and an inhibitor of bioactive IL-12p70 heterodimer. Blood 97, 3466–3569.