Significant hybridization differences in comparative genomic hybridization due to nucleotides used for DNA labelling and to DNA chosen for cohybridization

Pathobiology

Volumn 70, Issue 4, 2002, Pages 204-208

  Wilkens, Ludwig ^a   Tchinda, Joelle ^b   Burkhardt, Dagmar ^a   Kreipe, Hans Heinrich ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Artifacts; Comparative genomic hybridization; DNA; Fluorescence in situ hybridization; Hybridization

Indexed keywords

DIGOXIGENIN; DNA; NUCLEOTIDE DERIVATIVE;

ARTICLE; BACTERIUM CONJUGATION; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; CYTOGENETICS; GENETIC ANALYSIS; HUMAN; IN SITU HYBRIDIZATION; KARYOTYPE; MEDICAL ASSESSMENT; PRIORITY JOURNAL; SAMPLING; SIGNAL TRANSDUCTION;

ADULT; ARTIFACTS; BONE MARROW CELLS; CELLS, CULTURED; CHROMOSOME ABERRATIONS; DNA; DNA PROBES; FEMALE; HUMANS; IN SITU HYBRIDIZATION; IN SITU HYBRIDIZATION, FLUORESCENCE; KARYOTYPING; LEUKEMIA, NONLYMPHOCYTIC, ACUTE; MALE; MIDDLE AGED; MYELODYSPLASTIC SYNDROMES; MYELOPROLIFERATIVE DISORDERS; REPRODUCIBILITY OF RESULTS;

EID: 0038675165     PISSN: 10152008     EISSN: None     Source Type: Journal    
DOI: 10.1159/000069330     Document Type: Article

References (20)

1. Knuutila S, Björkqvist A, Autio K, Tarkkanen M, Wolf M, Monni O, Szymanska J, Larramendy ML, Tapper J, Pere H, El-Rifai W, Hemmer S, Wasenius V, Vidgren V, Zhu Y: DNA copy number amplifications in human neoplasms. Review of comparative genomic hybridisation. Am J Pathol 1998;152:1107-1123.
2. Zitzelsberger H, Lehmann L, Werner M, Bauchinger M: Comparative genomic hybridisation for the analysis of chromosomal imbalances in solid tumours and haematological malignancies. Histochem Cell Biol 1997;108:403-417.
3. Karhu R, Kahkonen M, Kuukasjarvi T, Pennanen S, Tirkkonen M, Kallioniemi O: Quality control of CGH: Impact of metaphase chromosomes and the dynamic range of hybridization. Cytometry 1997;28:198-205.
4. Kallioniemi O, Kallioniemi A, Piper J, Isola J, Waldman F, Gray J, Pinkel D: Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumours. Genes Chromosome Cancer 1994;10:231-243.
5. El-Rifai W, Larramendy ML, Bjorkqvist A, Hemmer S, Knuutila S: Optimization of comparative genomic hybridization using fluorochrome conjugated to dCTP and dUTP nucleotides. Lab Invest 1997;77:699-700.
6. Larramendy ML, El-Rifai W, Knuutila S: Comparison of fluorescein isothiocyanate- and Texas red-conjugated nucleotides for direct labeling in comparative genomic hybridization. Cytometry 1998;31:174-179.
7. Wilkens L, Tchinda J, Burkhardt D, Nolte M, Werner M, Georgii A: Analysis of hematologic diseases using conventional karyotyping, fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). Hum Pathol 1998;29:833-839.
8. Werner M, Nolte M, Kaloutsi V, Buhr T, Kausche F, Georgii A: Karyotype findings and molecular analysis of the bcr gene rearrangement supplementing the histologic classification of chronic myeloproliferative disorders. Lab Invest 1995;72:405-410.
9. Gosden CM, Davidson C, Robertson M: Lymphocyte culture; in Rooney DE, Czepulkowski BH (eds): Human cytogenetics: A practical approach. Oxford, Oxford University Press, 1992, pp 31-54.
10. Sambrook C, Fritsch EF, Maniatis T: Molecular cloning: A laboratory manual, ed 2. New York, Cold Spring Harbor Laboratory Press, 1989.
11. duManoir S, Speicher MR, Joos S, Schrock E, Popp S, Dohner H, Kovacs G, Robert-Nicoud M, Lichter P, Cremer T: Detection of complete and partial chromosome gains and losses by comparative genomic hybridization. Hum Genet 1993;90:590-610.
12. Sheehan C, Weiss AS: Yeast artificial chromosome: Rapid extraction for high resolution analysis. Nucleic Acids Res 1990;18:2193.
13. Nolte M, Werner M, von Wasielewski R, Nietgen G, Wilkens L, Georgii A: Detection of numerical karyotype changes in the giant cells of Hodgkin's lymphomas by a combination of FISH and immunohistochemistry applied to paraffin sections. Histochem Cell Biol 1996;105:401-404.
14. Ward BE, Gersen SL, Carelli MP, McGuire NM, Dackowski WR, Weinstein M, Sandlin C, Warren R, Klinger KW: Rapid prenatal diagnosis of chromosomal aneuploidies by fluorescence in situ hybridization: Clinical experience with 4,500 specimens. Am J Hum Genet 1993;52:854-865.
15. duManoir S, Kallioniemi OP, Lichter P, Piper J, Benedetti PA, Carothers AD, Fantes JA, Garcia JA, Giollant M: Hardware and software requirements for quantitative analysis of comparative genomic hybridization. Cytometry 1995;19:4-9.
16. Bryndorf T, Kichhoff M, Rose H, Maahr J, Gerdes T, Karhu R, Kallioniemi A, Christensen B, Lundsteen C, Philip J: Comparative genomic hybridization in clinical cytogenetics. Am J Hum Genet 1995;57:1211-1220.
17. Saccone S, DeSario A, Valle G, Bernardi G: The highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. Proc Natl Acad Sci USA 1997;77:699-701.
18. duManoir S, Schroeck E, Bentz M, Speicher MR, Joos S, Ried T, Lichter P, Cremer T: Quantitative analysis of comparative genomic hybridization. Cytometry 1995;19:27-41.
19. Korenberg JR, Rykowski MC: Human genome organization: Alu lines and the molecular structure of metaphase chromosome bands. Cell 1988;53:391-400.
20. Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D: Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 1992;258:818-821.