Overexpression of thermoalkalophilic lipase from Bacillus stearothermophilus L1 in Saccharomyces cerevisiae

Journal of Microbiology and Biotechnology

Volumn 13, Issue 3, 2003, Pages 451-456

  Ahn, Jung Oh ^a   Jang, Hyung Wook ^b   Lee, Hong Weon ^c   Choi, Eui Sung ^c   Haam, Seung Joo ^a   Oh, Tae Kwang ^c   Jung, Joon Ki ^c  

^a Yonsei University   (South Korea)

^b Acebiotech Corporation   (South Korea)

^c Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Bacillus stearothermophilus L1; Induction time; Saccharomyces cerevisiae; Thermoalkalophilic lipase

Indexed keywords

AMYLASE; GALACTOSIDASE; LIPOPROTEIN LIPASE; RECOMBINANT ENZYME; SEPHAROSE; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

ARTICLE; ASPERGILLUS ORYZAE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FED BATCH CULTURE; GENE FUSION; GENE OVEREXPRESSION; GENE SEQUENCE; GENETIC CODE; GEOBACILLUS STEAROTHERMOPHILUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PLASMID; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; THERMOPHILIC BACTERIUM;

ASPERGILLUS; ASPERGILLUS ORYZAE; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; VECTORS;

EID: 0038574649     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Chung, B. H., B. M. Kim, S. K. Rhee, Y. H. Park, and S. W. Nam. 1995. Effect of galactose feeding strategy on heterologous human lipocortin-I production in the fed-batch culture of Saccharomyces cerevisiae controlled by the GAL10 promoter. J. Microbiol. Biotechnol. 5: 224-228.
2. De Deken, R. H. 1966. The Crabtree effect: A regulatory system in yeast. J. Gen. Microbiol. 44: 149-156.
3. El-enshasy, H., K. Hellmuth, and U. Rinas. 1999. GpdA-promoter-controlled production of glucose oxidase by recombinant Aspergillus niger nonglucose carbon sources. Appl. Biochem. Biotechnol. 90: 57-66.
4. Hellmuth, K., S. Pluschkell, J. K. Jung, E. Ruttkowski, and U. Rinos. 1995. Optimization of glucose oxidase production by Aspergillus niger using genetic- and process-engineering techniques. Appl. Microbiol. Biotechnol. 43: 978-984.
5. Jeong, K. J. and S. Y. Lee. 1999. High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification. Appl. Environ. Microbiol. 65: 3027-3032.
6. Jeong, S. T., H. K. Kim, S. J. Kim, J. G. Pan, T. K. Oh, and S. G. Ryu. 2001. Crystallization and preliminary X-ray analysis of thermoalkalophilic lipase from Bacillus stearothermophilus L1. Biologic. Crystal. D57: 1300-1302.
7. Johnston, M. 1987. A model fungal gene regulatory mechanism: The GAL genes of Saccharomyces cerevisiae. Microbial Re~ 51: 458-476.
8. Jung, W. H., H. K. Kim, C. Y. Lee, and T. K. Oh. 2002. Biochemical properties and substrate specificity of lipase from Staphylococcus aureus B56. J. Microbial. Biotechnol. 12: 25-30.
9. Kapat, A., J. K. Jung, and Y. H. Park. 2000. Effect of continuous feeding of galactose on the production of recombinant glucose oxidase using Saccharomyces cerevisiae. Bioproc. Eng. 23: 37-40.
10. Kim, H. K., S. Y. Park, J. K. Lee, and T. K. Oh. 1998. Gene cloning and characterization of thermostable lipase from Bacillus stearothermophilus L1. Biosci. Biotech. Biochem. 62: 66-71.
11. Kim, K. Y., M. D. Kim, S. H. Nam, and J. H. Seo. 2002. Display of Bacillus macerans cyclodextrin glucanotransferase on cell surface of Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 12: 411-416.
12. Kim, M. H., H. K. Kim, J. K. Lee, and T. K. Oh. 2000. Thermostable lipase of Bacillus stearothermophilus: High-level production, purification, and calcium-dependent thermostability. Biosci. Biotech. Biochem. 64: 280-286.
13. Kim, T. H., J. K. Jung, S. S. Kwak, S. W. Nam, M. J. Chun, and Y. H. Park. 2002. Heterologous expression and secretion of sweet potato peroxidase isoenzyme A1 in recombinant Saccharomyces cerevisiae. Biotechnol. Lett. 24: 279-286.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
15. Novick, P., S. Ferro, and R. Scheckman. 1985. Order events in the yeast secretory form yeast. Science 25: 461-469.
16. Romanos, M. A., C. A. Scorer, and J. J. Clare. 1992. Foreign gene expression in yeast: a review. Yeast 8: 423-488.
17. Rua, M. L., H. Atomi, C. Schmidt-Dannert, and R. D. Schmid. 1998. High-level expression of the thermoalkalophilic lipase from Bacillus thermocatenulatus in Escherichia coli. Appl. Microbiol. Biotechnol. 49: 405-410.
18. Schmidt-Dannert, C. 1999. Recombinant microbial lipase for biotechnological applications. Biol. Med. Chem. 7: 2123-2130.
19. Schmidt-Dannert, C., M. L. Rua, H. Atomi, and R. D. Schmid. 1996. Thermoalkalophilic lipase of Bacillus thermocatenulatus I. Molecular cloning, nucleotide sequence, purification and some properties. Biochim. Biophys. Acta 1301: 105-114.