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A pioneering effort to successfully increase monoterpene production in plants. A gene encoding an enzyme of the MEP pathway was overexpressed in peppermint. Also, antisense-mediated suppression of a cytochrome P450 hydroxylase reduced levels of the undesirable end product menthofuran.
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Chen D.H., Ye H.C., Li G.F. Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation. Plant Sci. 155:2000;179-185 The overexpression of a gene in a sagebrush species encoding an enzyme for the second phase of terpene biosynthesis, FPP synthase, resulted in a significant increase in the accumulation of artemisinin, a non-volatile sesquiterpene used in malaria treatment.
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This work demonstrates the enormous potential of altering terpene accumulation by the introduction of terpene synthase genes. The authors engineered the accumulation of the monoterpene linalool in ripening tomato fruits by transforming tomato with the monoterpene synthase, linalool synthase, under control of a specific promoter expressed in maturing fruit.
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Lewinsohn E., Schalechet F., Wilkinson J., Matsui K., Tadmor Y., Nam K.-H., Amar O., Lastochkin E., Larkov O., Ravid U.et al. Enhanced levels of the aroma and flavor compound S-linalool by metabolic engineering of the terpenoid pathway in tomato fruits. Plant Physiol. 127:2001;1256-1265 This work demonstrates the enormous potential of altering terpene accumulation by the introduction of terpene synthase genes. The authors engineered the accumulation of the monoterpene linalool in ripening tomato fruits by transforming tomato with the monoterpene synthase, linalool synthase, under control of a specific promoter expressed in maturing fruit.
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Introduction of the linalool synthase gene into carnation under the control of a constitutive promoter resulted in modest release of linalool as well as some oxidised derivatives.
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Lavy M., Zuker A., Lewinsohn E., Larkov O., Ravid U., Vainstein A., Weiss D. Linalool and linalool oxide production in transgenic carnation flowers expressing the Clarkia breweri linalool synthase gene. Mol. Breeding. 9:2002;103-111 Introduction of the linalool synthase gene into carnation under the control of a constitutive promoter resulted in modest release of linalool as well as some oxidised derivatives.
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Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-D-glucopyranoside
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Transformation of petunia with linalool synthase under the control of a constitutive promoter resulted in the accumulation of linalool as a glucoside derivative. Although otherwise well planned and executed, this work illustrates the pitfalls of genetic engineering when one is unaware of the potential for further metabolism.
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Lücker J., Bouwmeester H.J., Schwab W., Blaas J., van der Plas L.H.W., Verhoeven H.A. Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-D-glucopyranoside. Plant J. 27:2001;315-324 Transformation of petunia with linalool synthase under the control of a constitutive promoter resulted in the accumulation of linalool as a glucoside derivative. Although otherwise well planned and executed, this work illustrates the pitfalls of genetic engineering when one is unaware of the potential for further metabolism.
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The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damage
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Schnee C., Köllner T.G., Gershenzon J., Degenhardt J. The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damage. Plant. Physiol. 130:2002;2049-2060.
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Whittington D.A., Wise M.L., Urbansky M., Coates R.M., Croteau R.B. Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase. Proc. Natl. Acad. Sci. USA. 99:2002;15375-15380.
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