Human recombinant endopeptidase PHEX has a strict S′1 specificity for acidic residues and cleaves peptides derived from fibroblast growth factor-23 and matrix extracellular phosphoglycoprotein

Biochemical Journal

Volumn 373, Issue 1, 2003, Pages 271-279

  Campos, Marcelo ^a   Couture, Constance ^b   Hirata, Izaura Y ^a   Juliano, Maria A ^a   Loisel, Thomas P ^c   Crine, Philippe ^b,c   Juliano, Luiz ^a   Boileau, Guy ^b,c   Carmona, Adriana K ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

^c INSTITUTE FOR RESEARCH IN IMMUNOLOGY AND CANCER   (Canada)

Author keywords

Combinatorial library; Internally quenched fluorogenic substrate; M13 endopeptidase; PHEX substrate specificity

Indexed keywords

FLUORESCENCE; HYDROLYSIS; PROTEINS; SUBSTRATES;

ACIDIC RESIDUES;

GENES;

2,4 DINITROPHENOL; ANTHRANILIC ACID; ANTHRANILIC ACID GLYCYLPHENYLALANYLSERYLASPARTYLTYROSYLLYSYLDINITROPHENYLALANINE; ASPARTIC ACID; ETHYLENEDIAMINE DERIVATIVE; FIBROBLAST GROWTH FACTOR; FIBROBLAST GROWTH FACTOR 23; GLUTAMIC ACID; GLYCINE; GLYCOPROTEIN; HISTIDINE; ISOLEUCINE; LEUCINE; MATRIX EXTRACELLULAR PHOSPHOGLYCOPROTEIN; N (2,4 DINITROPHENYL)ETHYLENEDIAMINE; PEPTIDE; PEPTIDE LIBRARY; PROLINE; RECOMBINANT ENDOPEPTIDASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; VALINE;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FLUORESCENCE; GENE; HUMAN; MICHAELIS CONSTANT; PHEX GENE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CHROMOSOMES, HUMAN, X; ENDOPEPTIDASES; FIBROBLAST GROWTH FACTORS; GLYCINE; HUMANS; HYPOPHOSPHATEMIA; KINETICS; LEUCINE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PEPTIDE FRAGMENTS; PHEX PHOSPHATE REGULATING NEUTRAL ENDOPEPTIDASE; PROLINE; PROTEINS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 0038343128     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030287     Document Type: Article

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