Yeast-based genetic system for functional analysis of poxvirus mRNA cap methyltransferase

Journal of Virology

Volumn 77, Issue 13, 2003, Pages 7300-7307

  Saha, Nayanendu ^a   Shuman, Stewart ^b   Schwer, Beate ^a,b  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAPPED RNA; CAPPED RNA METHYLTRANSFERASE; GUANINE N7 METHYLTRANSFERASE; GUANYLYLTRANSFERASE; METHYLTRANSFERASE; TRIPHOSPHATASE; UNCLASSIFIED DRUG; VIRUS ENZYME;

ARTICLE; CATALYSIS; CELL GROWTH; DIMERIZATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBUNIT; GENETIC COMPLEMENTATION; MUTATION; NONHUMAN; POXVIRUS; PRIORITY JOURNAL; PROTEIN DOMAIN; SACCHAROMYCES CEREVISIAE; VACCINIA VIRUS; VIROGENESIS;

AMINO ACID SEQUENCE; GENETIC COMPLEMENTATION TEST; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; PLASMIDS; POXVIRIDAE; RNA CAPS; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID; VIRAL PROTEINS;

EID: 0038339628     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.13.7300-7307.2003     Document Type: Article

References (42)

1. Bentley, D. 2002. The mRNA assembly line: transcription and processing machines in the same factory. Curr. Opin. Cell Biol. 14:336-343.
2. Borchardt, R. T., B. T. Keller, and U. Patel-Thombre. 1984. Neplanocin A: a potent inhibitor of S-adenosylhomocysteine hydrolase and of vaccinia virus multiplication in mouse L929 cells. J. Biol. Chem. 259:4353-4358.
3. Broyles, S. S., and B. Moss. 1986. Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequences and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa sub-units. Proc. Natl. Acad. Sci. USA 83:3141-3145.
4. Bujnicki, J. M., M. Feder, M. Radlinska, and L. Rychlewski. 2001. mRNA guanine-N7 cap methyltransferases: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure relationships. BMC Bioinformatics 2:2.
5. 7GpppG binding to the nuclear cap-binding complex. Nat. Struct. Biol. 9:912-917.
6. Carpenter, M. S., and A. M. DeLange. 1991. A temperature-sensitive lesion in the small subunit of vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution. J. Virol. 65:4042-4050.
7. Cong, P., and S. Shuman. 1992. Methyltransferase and subunit association domains of vaccinia virus mRNA capping enzyme. J. Biol. Chem. 267:16424-16429.
8. Cong, P., and S. Shuman. 1993. Covalent catalysis in nucleotidyl transfer: a KTDG motif essential for enzyme-GMP complex formation by mRNA capping enzyme is conserved at the active sites of RNA and DNA ligases. J. Biol. Chem. 268:7256-7260.
9. Cong. P., and S. Shuman. 1995. Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate complex formation, and GMP transfer to RNA. Mol. Cell. Biol. 15:6222-6231.
10. De Clercq, E. 2001. Vaccinia virus inhibitors as a paradigm for the chemotherapy of poxvirus infections. Clin. Microbiol. Rev. 14:382-397.
11. Fabrega, C., V. Shen, S. Shuman, and C. D. Lima. Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II. Mol. Cell, in press.
12. Gong, C., and S. Shuman. Mapping the active site of vaccinia virus RNA triphosphatase. Virology, in press.
13. Hassett, D. E., J. I. Lewis, X. Xing, L. DeLange, and R. C. Condit. 1997. Analysis of a temperature-sensitive vaccinia virus mutant in the viral mRNA capping enzyme isolated by clustered change-to-alanine mutagenesis and transient dominant selection. Virology 238:391-409.
14. Hausmann, S., C. P. Vivars, and S. Shuman. 2002. Characterization of the mRNA capping apparatus of the microsporidian parasite Encephalitozoon cuniculi. J. Biol. Chem. 277:96-103.
15. Higman, M. A., N. Bourgeois, and E. G. Niles. 1992. The vaccinia virus mRNA (guanine-N7-) methyltransferase requires both subunits of the mRNA capping enzyme for activity. J. Biol. Chem. 267:16430-16437.
16. Higman, M. A., L. A. Christen, and E. G. Niles. 1994. The mRNA (guanine-7-) methyltransferase domain of the vaccinia virus mRNA capping enzyme: expression in Escherichia coli and structural and kinetic comparison to the intact capping enzyme. J. Biol. Chem. 269:14974-14981.
17. Higman, M. A., and E. G. Niles. 1994. Location of the S-adenosyl-L-methionine binding region of the vaccinia virus mRNA (guanine-7-) methyltransferase. J. Biol. Chem. 269:14982-14987.
18. Ho, C. K., A. Martins, and S. Shuman. 2000. A yeast-based genetic system for functional analysis of viral mRNA capping enzymes. J. Virol. 74:5486-5494.
19. Luo, Y., X. Mao, L. Deng, P. Cong, and S. Shuman. 1995. The D1 and D12 subunits are both essential for the transcription termination factor activity of vaccinia virus capping enzyme. J. Virol. 69:3852-3856.
20. Mao, X., and S. Shuman. 1994. Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit: identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer. J. Biol. Chem. 269:24472-24479.
21. Mao, X., and S. Shuman. 1996. Vaccinia virus mRNA (guanine-7-) methyltransferase: mutational effects on cap methylation and AdoHcy-dependent photocrosslinking of the cap to the methyl acceptor site. Biochemistry 35:6900-6910.
22. Mao, X., B. Schwer, and S. Shuman. 1995. Yeast mRNA cap methyltransferase is a 50-kilodalton protein encoded by an essential gene. Mol. Cell. Biol. 15:4167-4174.
23. Mao, X., B. Schwer, and S. Shuman. 1996. Mutational analysis of the Saccharomyces cerevisiae ABD1 gene: cap methyltransferase activity is essential for cell growth. Mol. Cell. Biol. 16:475-480.
24. Martin, S. A., and B. Moss. 1975. Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions. J. Biol. Chem. 250:9330-9335.
25. McCracken, S., N. Fong, E. Rosonina, K. Yankulov, G. Brothers, D. Siderovski, A. Hessel, S. Foster, S. Shuman, and D. L. Bentley. 1997. 5′ Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated C-terminal domain of RNA polymerase II. Genes Dev. 11:3306-3318.
26. Myette, J., and E. G. Niles. 1996. Domain structure of the vaccinia virus mRNA capping enzyme: expression in Escherichia coli of a subdomain possessing the RNA 5′-triphosphatase and guanylyltransferase activities and a kinetic comparison to the full-size enzyme. J. Biol. Chem. 271:11936-11944.
27. Myette, J., and E. G. Niles. 1996. Characterization of the vaccinia virus RNA 5′-triphosphatase and nucleoside triphosphate phosphohydrolase activities: demonstration that both activities are carried out at the same active site. J. Biol. Chem. 271:11945-11952.
28. Niles, E. G., and L. Christen. 1993. Identification of the vaccinia virus mRNA guanylyltransferase active site lysine. J. Biol. Chem. 268:24986-24989.
29. Niles, E. G., L. Christen, and M. A. Higman. 1994. Direct photolinkage of GTP to the vaccinia virus mRNA (guanine-7-) methyltransferase GTP methyl acceptor site. Biochemistry 33:9898-9903.
30. Saha, N., B. Schwer, and S. Shuman. 1999. Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes. J. Biol. Chem. 274:16553-16562.
31. Saha, N., and S. Shuman. 2001. Effects of alanine cluster mutations in the D12 subunit of vaccinia virus mRNA (guanine-N7) methyltransferase. Virology 287:40-48.
32. Schwer, B., N. Saha, X. Mao, H. W. Chen, and S. Shuman. 2000. Structure-function analysis of yeast mRNA cap methyltransferase and high-copy suppression of conditional mutants by AdoMet synthase and the ubiquitin conjugating enzyme Cdc34p. Genetics 155:1561-1576.
33. Shuman, S. 1989. Functional domains of vaccinia virus mRNA capping enzyme: analysis by limited tryptic digestion. J. Biol. Chem. 264:9690-9695.
34. Shuman, S. 2000. Structure, mechanism, and evolution of the mRNA capping apparatus. Prog. Nucleic Acid Res. Mol. Biol. 66:1-40.
35. Shuman, S. 2001. The mRNA capping apparatus as drug target and guide to eukaryotic phylogeny. Cold Spring Harbor Symp. Quant. Biol. 66:301-312.
36. Shuman, S., S. Broyles, and B. Moss. 1987. Purification and characterization of a transcription termination factor from vaccinia virions. J. Biol. Chem. 262:12372-12380.
37. Shuman, S., and S. G. Morham. 1990. Domain structure of vaccinia virus mRNA capping enzyme: activity of the Mr 95,000 subunit expressed in Escherichia coli. J. Biol. Chem. 265:11967-11972.
38. Venkatesan, S., A. Gershowitz, and B. Moss. 1980. Modification of the 5′ end of mRNA: association of RNA triphosphatase with the RNA guanylyltransferase-RNA (guanine-7) methyltransferase complex from vaccinia virus. J. Biol. Chem. 255:903-908.
39. Vos, J. C., M. Sasker, and H. G. Stunnenberg. 1991. Vaccinia virus capping enzyme is a transcription initiation factor. EMBO J. 10:2553-2558.
40. Wang, S. P., and S. Shuman. 1997. Structure-function analysis of the mRNA cap methyltransferase of Saccharomyces cerevisiae. J. Biol. Chem. 272:14683-14689.
41. Yu, L., and S. Shuman. 1996. Mutational analysis of the triphosphatase domain of vaccinia virus mRNA capping enzyme. J. Virol. 70:6162-6168.
42. Yu, L., A. Martins, L. Deng, and S. Shuman. 1997. Structure-function analysis of the triphosphatase component of vaccinia virus mRNA capping enzyme. J. Virol. 71:9837-9843.