Effects of alanine cluster mutations in the D12 subunit of vaccinia virus mRNA (guanine-N7) methyltransferase

Virology

Volumn 287, Issue 1, 2001, Pages 40-48

  Saha, Nayanendu ^a   Shuman, Stewart ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; METHYLTRANSFERASE; MUTANT PROTEIN; VIRUS ENZYME; VIRUS RNA;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME STRUCTURE; ENZYME SUBUNIT; METHYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; VACCINIA VIRUS; VIRUS MUTATION;

EID: 0035881512     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2001.1006     Document Type: Article

References (25)

1. Methyltransferase and subunit association domains of vaccinia virus mRNA capping enzyme
2. Covalent catalysis in nucleotidyl transfer: A KTDG motif essential for enzyme-GMP complex formation by mRNA capping enzyme is conserved at the active sites of RNA and DNA ligases
3. Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate complex formation, and GMP transfer to RNA
4. The vaccinia virus mRNA (guanine-N7-) methyltransferase requires both subunits of the mRNA capping enzyme for activity
5. The mRNA (guanine-7-) methyltransferase domain of the vaccinia virus mRNA capping enzyme: Expression in Escherichia coli and structural and kinetic comparison to the intact capping enzyme
6. Location of the S-adenosyl-l-methionine binding region of the vaccinia virus mRNA (guanine-7-) methyltransferase
7. A yeast-based genetic system for functional analysis of viral mRNA capping enzymes
8. RNA binding properties of vaccinia virus capping enzyme
9. Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit. Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer
10. Vaccinia virus mRNA (guanine-7-) methyltransferase: Mutational effects on cap methylation and Ado Hcy-dependent photocrosslinking of the cap to the methyl acceptor site
11. Yeast mRNA cap methyltransferase is a 50-kDa protein encoded by an essential gene
12. Mutational analysis of the Saccharomyces cerevisiae ABD1 gene: Cap methyltransferase activity is essential for cell growth
13. Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions
14. Domain structure of the vaccinia virus mRNA capping enzyme: Expression in Escherichia coli of a subdomain possessing the RNA 5′-triphosphatase and guanylyltransferase activities and a kinetic comparison to the full-size enzyme
15. Characterization of the vaccinia virus RNA 5′-triphosphatase and nucleoside triphosphate phosphohydrolase activities: Demonstration that both activities are carried out at the same active site
16. Identification of the vaccinia virus mRNA guanylyltransferase active site lysine
17. Direct photolinkage of GTP to the vaccinia virus mRNA (guanine-7-) methyltransferase GTP methyl acceptor site
18. African swine fever virus guanylyltransferase
19. Characterization of human, Schizosaccharomyces pombe and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes
20. Functional domains of vaccinia virus mRNA capping enzyme: Analysis by limited tryptic digestion
21. Domain structure of vaccinia virus mRNA capping enzyme: Activity of the Mr 95000 subunit expressed in Escherichia coli
22. Modification of the 5′ end of mRNA: Association of RNA triphosphatase with the RNA guanylyltransferase-RNA (guanine-7) methyltransferase complex from vaccinia virus
23. Structure-function analysis of the mRNA cap methyltransferase of Saccharomyces cerevisiae
24. Mutational analysis of the triphosphatase domain of vaccinia virus mRNA capping enzyme
25. Structure-function analysis of the triphosphatase component of vaccinia virus mRNA capping enzyme