Cloning and expression of calglandulin, a new EF-hand protein from the venom glands of Bothrops insularis snake in E. coli

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1648, Issue 1-2, 2003, Pages 90-98

  Junqueira De Azevedo, Inácio De L M ^a,b   Pertinhez, Thelma ^c   Spisni, Alberto ^c,d   Carreño, Flávia Regina ^b   Farah, Chuck S ^b   Ho, Paulo Lee ^a,b  

^a INSTITUTO BUTANTAN   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c LABORATÓRIO NACIONAL DE LUZ SÍNCROTRON   (Brazil)

^d UNIVERSITY OF PARMA   (Italy)

Author keywords

Bothrops insularis; Ca2+ binding protein; Calglandulin; EF hand; Expressed sequence tag; Venom gland

Indexed keywords

ANTISERUM; CALCIUM BINDING PROTEIN; CALGLANDULIN; CALMODULIN; HYBRID PROTEIN; NICKEL; PROTEIN; PROTEIN DERIVATIVE; UNCLASSIFIED DRUG; VENOM; CALGLANDULIN, BOTHROPS INSULARIS; RECOMBINANT PROTEIN; SNAKE VENOM;

AFFINITY CHROMATOGRAPHY; ALPHA HELIX; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BOTHROPS INSULARIS; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; EXPERIMENTAL TEST; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC CODE; IMMUNOREACTIVITY; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTEIN PURIFICATION; SAMPLING; SNAKE; SPECTROSCOPY; TISSUE SLICE; WESTERN BLOTTING; ANIMAL; ANTIBODY SPECIFICITY; BIOSYNTHESIS; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR GENETICS; PHYLOGENY; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT;

BOTHROPS; BOTHROPS INSULARIS; ESCHERICHIA COLI; SERPENTES; VIPERIDAE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BOTHROPS; CLONING, MOLECULAR; CROTALID VENOMS; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; PHYLOGENY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

EID: 0038305524     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-9639(03)00111-0     Document Type: Article

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