Functional differentiation and selective inactivation of multiple Saccharomyces cerevisiae genes involved in very-long-chain fatty acid synthesis

Molecular Genetics and Genomics

Volumn 269, Issue 2, 2003, Pages 290-298

  Rossler H ^a   Rieck, C ^a   Delong, T ^a   Hoja, U ^a   Schweizer, E ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

Fatty acid elongation; Fatty acid synthesis; Very long chain fatty acids; Yeast

Indexed keywords

ACETYL COENZYME A; CERULENIN; ELONGASE 1; ELONGASE 2; ELONGASE 3; FATTY ACID; FATTY ACID SYNTHASE; FUNGAL ENZYME; HYDROXYACID; PALMITOYL COENZYME A; STEAROYL COENZYME A; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; FATTY ACID SYNTHESIS; FUNGAL GENETICS; FUNGAL STRAIN; GENE INACTIVATION; GENE MUTATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; YEAST;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0038280115     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-003-0836-0     Document Type: Article

References (24)

1. Beaudoin F, Michaelson LV, Hey SJ, Lewis MJ, Shewy PR, Sayanova O, Napier JA (2000) Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway. Proc Natl Acad Sci USA 97:6421-6426
2. Beaudoin F, Gable K, Sayanova O, Dunn T, Napier J (2002) A Saccharomyces cerevisiae gene required for heterologous fatty acid elongase activity encodes a microsomal β-keto-reductase. J Biol Chem 227:11481-11488
3. Bloch K, Vance D (1977) Control mechanisms in the synthesis of saturated fatty acids. Annu Rev Biochem 46:263-298
4. Brody S, Oh C, Hoja U, Schweizer E (1997) Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae. FEBS Lett 408:217-220
5. Cassagne C, Darriet D, Bourre JM (1978) Biosynthesis of very long chain fatty acids by the sciatic nerve of the rabbit. FEBS Lett 90:336-340
6. Dittrich F, Zajonc D, Hühne K, Hoja U, Ekici A, Greiner E, Klein H, Hofmann J, Bessoule JJ, Sperling P, Schweizer E (1998) Fatty acid elongation in yeast. Biochemical characteristics of the enzyme system and isolation of elongation-defective mutants. Eur J Biochem 252:477-485
7. Douglas WJ, Lim E, Keller J, Plooy I, Ralston E, Dooner HK (1995) Directed tagging of the Arabidopsis FATTY ACID ELONGATION 1 (FAE1) gene with the maize transposon Activator. Plant Cell 7:309-319
8. Gari E, Piedrafita L, Aldea M, Herrero E (1997) A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13:837-848
9. Han G, Gable K, Kohlwein S, Beaudoin F, Napier J, Dunn TM (2002) The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase. J Biol Chem 277:35440-35449
10. Kohlwein S, Eder S, Oh CS, Martin CE, Gable K, Bacikova, D, Dunn T (2001) Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae. Mol Cell Biol 21:109-125
11. Kresze GB, Steber L, Oesterhelt D, Lynen F (1977) Reaction of yeast fatty acid synthetase with iodoacetamide. 3. Malonyl-Coenzyme A decarboxylase as product of the reaction of fatty acid synthase with iodoacetamide. Eur J Biochem 79:191-199
12. Lynen F (1969) Yeast fatty acid synthase. Methods Enzymol 14:17-33
13. Millar AA, Kunst L (1997) Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. Plant J 12:121-131
14. Milton AM, Axelrod AE, Hofmann K (1950) The synthesis of higher aliphatic β-keto acids. J Am Chem Soc 72:1231-1232
15. Oh CS, Toke DA, Mandala S, Martin CE (1997) EL02 and EL03, homologs of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272:17376-17384
16. Omura S (1976) The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev 40:681-697
17. Schneiter R, Kohlwein S (1997) Organelle structure, function, and inheritance in yeast: a role for fatty acid synthesis? Cell 88:431-434
18. Schneiter R, Guerra CE, Lampl M, Gogg G, Kohlwein S, Klein HL (1999) The Saccharomyces cerevisiae hyperrecombination mutant hpr1Δ is synthetically lethal with two conditional alleles of the acetyl coenzyme A carboxylase gene and causes a defect in nuclear export of polyadenylated RNA. Mol Cell Biol 19:3415-3422
19. Todd J, Post-Beittenmiller D, Jaworski JG (1999) KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana. Plant J 17:119-130
20. Toke DA, Martin CE (1996) Isolation and characterization of a gene affecting fatty acid elongation in Saccharomyces cerevisiae. J Biol Chem 271:18413-18422
21. Wach A, Brachat A, Alberti-Segni C, Rebischung C, Philippsen P (1997) Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 13:1065-1075
22. Welch JW, Burlingame AL (1973) Very long chain fatty acids in yeast. J Bacteriol 115:464-466
23. Yalpani M, Willecke K, Lynen F (1969) Triacetic acid lactone, a derailment product of fatty acid biosynthesis. Eur J Biochem 8:495-502
24. Yephremov A, Wisman E, Huijser P, Huijser C, Wellesen K, Saedler H (1999) Characterization of the FIDDLEHEAD gene of Arabidopsis reveals a link between adhesion response and cell differentiation in the epidermis. Plant Cell 11: 2187-2201