Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases

Microbiology

Volumn 149, Issue 7, 2003, Pages 1647-1658

  Acharya, Narottam ^a   Kumar, Pradeep ^a   Varshney, Umesh ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSINE; DEOXYURIDINE PHOSPHATE; DNA; DNA POLYMERASE; ENZYME INHIBITOR; POLYPEPTIDE; PROMUTAGEN; UNCLASSIFIED DRUG; URACIL; URACIL DNA GLYCOSYLTRANSFERASE; URACIL DNA GLYCOSYLTRANSFERASE INHIBITOR; UREA;

ANIMAL CELL; ARTICLE; BACTERIOPHAGE; COMPLEX FORMATION; CONTROLLED STUDY; DEAMINATION; DISSOCIATION; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; EXPERIMENTAL MODEL; HERPES SIMPLEX VIRUS; HYDROPHOBICITY; INCUBATION TIME; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PATHOGENICITY; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN STRUCTURE;

ACTINOBACTERIA (CLASS); ANIMALIA; BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; ESCHERICHIA COLI; HERPES; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; SIMPLEXVIRUS; UNCULTURED ACTINOMYCETE;

EID: 0038148669     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.26228-0     Document Type: Article

References (36)

1. Acharya, N. & Varshney, U. (2002). Biochemical properties of single stranded DNA binding protein from Mycobacterium smegmatis, a fast growing mycobacterium and its physical and functional interaction with uracil DNA glycosylases. J Mol Biol 318 1251-1264.
2. Acharya, N., Roy, S. & Varshney, U. (2002). Mutational analysis of the uracil DNA glycosylase inhibitor protein and its interaction with Escherichia coli uracil DNA glycosylase. J Mol Biol 321 579-590.
3. Aravind, L. & Koonin, E. V. (2000). The α/β fold uracil DNA glycosylases: a common origin with diverse fates. Genome Biol 1, 1-8.
4. Bennett, S. E. & Mosbaugh, D. W. (1992). Characterization of the Escherichia coli uracil-DNA glycosylase inhibitor protein complex. J Biol Chem 267, 22512-22521.
5. Bennett, S. E., Schimerlik, M. I. & Mosbaugh, D. W. (1993). Kinetics of the uracil-DNA glycosylase/inhibitor protein association. Ung interaction with Ugi, nucleic acids, and uracil compounds. J Biol Chem 268, 26879-26885.
6. Chen, J. D. & Lacks, S. A. (1991). Role of uracil-DNA glycosylase in mutation avoidance by Streptococcus pneumoniae. J Bacteriol 173, 283-290.
7. Cole, S. T., Brosch, R., Parkhill, J. & 39 other authors (1998). Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537-544.
8. Cone, R., Bonura, T. & Friedberg, E. C. (1980). Inhibitor of uracil-DNA glycosylase induced by bacteriophage PBS2. Purification and preliminary characterization. J Biol Chem 255, 10354-10358.
9. Duncan, B. K. & Weiss, B. (1982). Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli. J Bacteriol 151, 750-755.
10. Handa, P., Roy, S. & Varshney, U. (2001). The role of leucine 191 of Escherichia coli in forming a stable complex with a substrate mimic, Ugi, and in uracil excision from synthetic substrates. J Biol Chem 276, 17324-17331.
11. Handa, P., Acharya, N., Talawar, R. K., Roy, S. & Varshney, U. (2002). Contrasting effects of mutating active site residues, aspartic acid 64 and histidine 187 of Escherichia coli uracil-DNA glycosylase on uracil excision and interaction with an inhibitor protein. Ind J Biochem Biophys 29, 312-317.
12. Impellizzeri, K. J., Anderson, B. & Burgers, P. M. (1991). The spectrum of spontaneous mutations in a Saccharomyces cerevisiae uracil-DNA-glycosylase mutant limits the function of this enzyme to cytosine deamination repair. J Bacteriol 21, 6807-6810.
13. Krokan, H. E., Standal, R. & Slupphaug, G. (1997). DNA glycosylases in the base excision repair of DNA. Biochem J 325, 1-15.
14. Kumar, N. V. & Varshney, U. (1994). Inefficient excision of uracil from loop regions of DNA oligomers by E. coli uracil-DNA glycosylase. Nucleic Acids Res 18, 3737-3741.
15. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
16. Lindahl, T. (1974). An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues. Proc Natl Acad Sci U S A 71, 3649-3653.
17. Lundquist, A. J., Beger, R. D., Bennett, S. E., Bolton, P. H. & Mosbaugh, D. W. (1997). Site-directed mutagenesis and characterization of uracil-DNA glycosylase inhibitor protein. Role of specific carboxylic amino acids in complex formation with Escherichia coli uracil-DNA glycosylase. J Biol Chem 272, 21408-21419.
18. Millins, A. K., Carpenter, M. S. & DeLange, A. M. (1994). The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication. Virology 198, 504-513.
19. Mol, C. D., Arvai, A. S., Sanderson, R. J., Slupphaug, G., Kavli, B., Krokan, H. E., Mosbaugh, D. W. & Tainer, J. A. (1995). Crystal structure of the human Uracil-DNA glycosylase in complex with a protein inhibitor: protein mimicry of DNA. Cell 82, 701-708.
20. Mullaney, J., Moss, H. W. & McGeoch, D. J. (1989). Gene UL2 of herpes simplex virus type 1 encodes a uracil-DNA glycosylase. J Gen Virol 70, 449-454.
21. Nilsen, H., Rosewell, I., Robins, P. & 7 other authors (2000). Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication. Mol Cell 5, 1059-1065.
22. Olsen, L. C., Aasland, R., Krokan, H. E. & Helland, D. E. (1991). Human uracil-DNA glycosylase complements E. coli ung mutants. Nucleic Acids Res 19, 4473-4478.
23. Purnapatre, K., Handa, P., Venkatesh, J. & Varshney, U. (1999). Differential effects of single-stranded DNA binding proteins (SSBs) on uracil-DNA glycosylases (UDGs) from Escherichia coli and mycobacteria. Nucleic Acids Res 27, 3487-3492.
24. Putnam, C. D., Shroyer, M. J. N., Lundquist, A. J., Mol, C. D., Arvai, A. S. & Mosbaugh, D. W. (1999). Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase. J Mol Biol 287, 331-346.
25. Pyles, R. B. & Thompson, R. L. (1994). Evidence that the herpes simplex virus type 1 uracil DNA glycosylase is required for efficient viral replication and latency in the murine nervous system. J Virol 68, 4963-4972.
26. Ravishankar, R., Bidya Sagar, M., Roy, S., Purnapatre, K., Handa, P., Varshney, U. & Vijayan, M. (1998). X-ray analysis of a complex of E. coli uracil-DNA glycosylase (EcUDG) with its proteinaceous inhibitor. The structure elucidation of a prokaryotic UDG. Nucleic Acids Res 26, 4880-4887.
27. Roy, S., Purnapatre, K., Handa, P., Boyanapalli, M. & Varshney, U. (1998). Use of coupled transcriptional system for consistent overexpression and purification of UDG-Ugi complex and Ugi from E. coli. Protein Expr Purif 13, 155-162.
28. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
29. Sasaki, Y., Ishikawa, J., Yamashita, A., Oshima, K., Kenri, T., Furuya, K., Yoshino, C., Horino, A., Shiba, T., Sasaki, T. & Hattori, M. (2002). The complete genomic sequence of Mycoplasma penetrans, an intracelhilar bacterial pathogen in humans. Nucleic Acids Res 30, 5293-5300.
30. Savva, R. & Pearl, L. H. (1995). Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex. Nat Struct Biol 2, 752-755.
31. Sedmak, J. J. & Grossberg, S. E. (1977). A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem 79, 544-552.
32. Snapper, S. B., Melton, R. E., Mustafa, S., Kieser, T. & Jacobs, W. R., Jr (1990). Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol Microbiol 4, 1911-1919.
33. Stuart, D. T., Upton, C., Higman, M. A., Niles, E. G. & McFadden, G. (1993). A poxvirus-encoded uracil DNA glycosylase is essential for virus viability. J Virol 67, 2503-2512.
34. Wang, Z. & Mosbaugh, D. W. (1989). Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a binding protein specific for uracil-DNA glycosylase. J Biol Chem 264, 1163-1171.
35. Willetts, K. E., Rey, F., Agostini, I., Navarro, J. M., Baudat, Y., Vigne, R. & Sire, J. (1999). DNA repair enzyme uracil DNA glycosylase is specifically incorporated into human immunodeficiency virus type 1 viral particles through a Vpr-independent mechanism. J Virol 73, 1682-1688.
36. Williams, M. V. & Pollack, J. D. (1990). A mollicute (mycoplasma) DNA repair enzyme: purification and characterization of uracil-DNA glycosylase. J Bacteriol 172, 2979-2985.