Cigarette smoke extract induces oxidative stress and apoptosis in human lung fibroblasts

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 284, Issue 6 28-6, 2003, Pages

  Carnevali, Stefano ^a,c   Petruzzelli, Stefano ^a   Longoni, Biancamaria ^a   Vanacore, Renato ^a   Barale, Roberto ^b   Cipollini, Monica ^b   Scatena, Fabrizio ^a   Paggiaro, Pierluigi ^a   Celi, Alessandro ^a   Giuntini, Carlo ^a  

^a UNIVERSITY OF PISA   (Italy)

^b CISANELLO HOSPITAL   (Italy)

^c Dipartimento Cardio Toracico   (Italy)

Author keywords

Comet assay

Indexed keywords

ACETYLCYSTEINE; BUTHIONINE SULFOXIMINE; CIGARETTE SMOKE; DNA FRAGMENT;

APOPTOSIS; ARTICLE; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; INCUBATION TIME; LUNG EMPHYSEMA; LUNG FIBROBLAST; OXIDATIVE STRESS; PRIORITY JOURNAL;

EID: 0037835443     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.00466.2001     Document Type: Article

References (40)

1. Aoshiba K, Yasui S, Nishimura K, and Nagai A. Thiol depletion induces apoptosis in cultured lung fibroblasts. Am J Respir Cell Mol Biol 21: 54-64, 1999.
2. Auerbach O, Hammond EC, Garfinkel L, and Benante C. Relation of smoking and age to emphysema. Whole-lung section study. N Engl J Med 286: 853-857, 1972.
3. Bernard GR, Lucht WD, Niedermeyer ME, Snapper JR, Ogletree ML, and Brigham KL. Effect of N-acetylcysteine on the pulmonary response to endotoxin in the awake sheep and upon in vitro granulocyte function. J Clin Invest 73: 1772-1784, 1984.
4. Brown LA. Glutathione protects signal transduction in type II cells under oxidant stress. Am J Physiol Lung Cell Mol Physiol 266: L172-L177, 1994.
5. Carnevali S, Nakamura Y, Mio T, Liu X, Takigawa K, Romberger DJ, Spurzem JR, and Rennard SI. Cigarette smoke extract inhibits fibroblast-mediated collagen gel contraction. Am J Physiol Lung Cell Mol Physiol 274: L591-L598, 1998.
6. Carp H and Janoff A. Possible mechanisms of emphysema in smokers. In vitro suppression of serum elastase-inhibitory capacity by fresh cigarette smoke and its prevention by antioxidants. Am Rev Respir Dis 118: 617-621, 1978.
7. Chandra J, Samali A, and Orrenius S. Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med 29: 323-333, 2000.
8. Choucroun P, Gillet D, Dorange G, Sawicki B, and Dewitte JD. Comet assay and early apoptosis. Mutat Res 478: 89-96, 2001.
9. Church T and Pryor WA. Free radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 64: 111-126, 1985.
10. Cotgreave IA. N-acetylcysteine: pharmacological considerations and experimental and clinical applications. Adv Pharmacol 38: 205-227, 1997.
11. Deneke SM and Fanburg BL. Regulation of cellular glutathione. Am J Physiol Lung Cell Mol Physiol 257: L163-L173, 1989.
12. Forkert PG. 1,1-Dichloroethylene-induced Clara cell damage is associated with in situ formation of reactive epoxide. Am J Respir Cell Mol Biol 20: 1310-1318, 1999.
13. Frenzilli G, Betti C, Davini T, Desideri M, Fornai E, Giannessi L, Maggiorelli F, Paoletti P, and Barale R. Evaluation of DNA damage in leukocytes of ex-smokers by single cell gel electrophoresis. Mutat Res 375: 117-123, 1997.
14. Godard T, Deslandes E, Lebailly P, Vigreux C, Poulain L, Sichel F, Poul JM, and Gauduchon P. Comet assay and DNA flow cytometry analysis of staurosporine-induced apoptosis. Cytometry 36: 117-122, 1999.
15. Godard T, Deslandes E, Lebailly P, Vigreux C, Sichel F, Poul JM, and Gauduchon P. Early detection of staurosporine induced apoptosis by comet and Annexin V assays. Histochem Cell Biol 112: 155-161, 1999.
16. Ishii T, Matsuse T, Igarashi H, Masuda M, Teramoto S, and Ouchi Y. Tobacco smoke reduces viability in human lung fibroblasts: protective effect of glutathione S-transferase P1. Am J Physiol Lung Cell Mol Physiol 280: L1189-L1195, 2001.
17. Jungas T, Motta I, Duffieux F, Fanen P, Stoven V, and Ojcius DM. Glutathione levels and BAX activation during apoptosis due to oxidative stress in cells expressing wild-type and mutant cystic fibrosis transmembrane conductance regulator. J Biol Chem 277: 27912-27918, 2002.
18. Kasahara Y, Tuder RM, Cool CD, Lynch DA, Flores SC, and Voelkel NF. Endothelial cell death and decreased expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 2 in emphysema. Am J Respir Crit Care Med 163: 737-744, 2001.
19. Kerr JF, Wyllie AH, and Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239-257, 1972.
20. Lahmouzi J, Simain-Sato F, Defresne MP, De Pauw MC, Heinen E, Grisar T, Legros JJ, and Legrand R. Effect of nicotine on rat gingival fibroblasts in vitro. Connect Tissue Res 41: 69-80, 2000.
21. Lee KW, Chung SY, Yang I, Lee Y, Ko EY, and Park MJ. Correlation of aging and smoking with air trapping at thinsection CT of the lung in asymptomatic subjects. Radiology 214: 831-836, 2000.
22. Longoni B, Boschi E, Demontis GC, Ratto GM, and Mosca F. Apoptosis and adaptive responses to oxidative stress in human endothelial cells exposed to cyclosporin A correlate with BCL-2 expression levels. FASEB J 15: 731-740, 2001.
23. Macho A, Hirsch T, Marzo I, Marchetti P, Dallaporta B, Susin SA, Zamzami N, and Kroemer G. Glutathione depletion is an early and calcium elevation is a late event of thymocyte apoptosis. J Immunol 158: 4612-4619, 1997.
24. MacNee W. Oxidants/antioxidants and COPD. Chest 117, Suppl 1: 303S-317S, 2000.
25. McConkey DJ, Hartzell P, Nicotera P, Wyllie AH, and Orrenius S. Stimulation of endogenous endonuclease activity in hepatocytes exposed to oxidative stress. Toxicol Lett 42: 123-130, 1988.
26. Muller T and Gebel S. The cellular stress response induced by aqueous extracts of cigarette smoke is critically dependent on the intracellular glutathione concentration. Carcinogenesis 19: 797-801, 1998.
27. Nakamura Y, Romberger DJ, Tate L, Ertl RF, Kawamoto M, Adachi Y, Mio T, Sisson JH, Spurzem JR, and Rennard SI. Cigarette smoke inhibits lung fibroblast proliferation and chemotaxis. Am J Respir Crit Care Med 151: 1497-1503, 1995.
28. Olive PL, Wlodek D, Durand RE, and Banàth P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp Cell Res 198: 259-267, 1992.
29. Rahman I and MacNee W. Role of transcription factors in inflammatory lung diseases. Thorax 53: 601-612, 1998.
30. Repine JE, Bast A, and Lankhorst I. Oxidative stress in chronic obstructive pulmonary disease. Oxidative stress study group. Am J Respir Crit Care Med 156: 341-357, 1997.
31. Robbiano L, Carrozzino R, Puglia CP, Corbu C, and Brambilla G. Correlation between induction of DNA fragmentation and micronuclei formation in kidney cells from rats and humans and tissue-specific carcinogenic activity. Toxicol Appl Pharmacol 161: 153-159, 1999.
32. Saetta M, Turat G, Maestrelli P, Mapp C, and Fabbri LM. Cellular and structural bases of chronic obstructive pulmonary disease. Am J Respir Crit Care Med 163: 1304-1309, 2001.
33. Sandstrom PA, Mannie MD, and Buttke TM. Inhibition of activation-induced death in T cell hybridomas by thiol antioxidants: oxidative stress as a mediator of apoptosis. J Leukoc Biol 55: 221-226, 1994.
34. Sato N, Iwata S, Nakamura K, Hori T, Mori K, and Yodoi J. Thiol-mediated redox regulation of apoptosis. Possible roles of cellular thiols other than glutathione in T cell apoptosis. J Immunol 154: 3194-3203, 1995.
35. Sherrill DL, Enright PL, Kaltenborn WT, and Lebowitz MD. Predictors of longitudinal change in diffusing capacity over 8 years. Am J Respir Crit Care Med 160: 1883-1887, 1999.
36. Skold CM, Andersson K, Hed J, and Eklund A. Short-term in vivo exposure to cigarette-smoke increases the fluorescence in rat alveolar macrophages. Eur Respir J 6: 1169-1172, 1993.
37. Snider GL. Emphysema: the first two centuries-and beyond. A historical overview, with suggestions for future research: part 1. Am Rev Respir Dis 146: 1334-1344, 1992.
38. Snider GL. Emphysema: the first two centuries-and beyond. A historical overview, with suggestions for future research: part 2. Am Rev Respir Dis 146: 1615-1622, 1992.
39. 2 toxicity by N-acetylcysteine. Eur Respir J 2: 116-126, 1989.
40. Wyllie AH, Kerr JF, and Currie AR. Cell death; the significance of apoptosis. Int Rev Cytol 68: 251-306, 1980.