Construction and high cytoplasmic expression of a tumoricidal single-chain antibody against hepatocellular carcinoma

BMC Biotechnology

Volumn 2, Issue , 2002, Pages

  Sandee, Duanpen ^a   Tungpradabkul, Sumalee ^b   Tsukio, Manae ^a   Imanaka, Tadayuki ^c   Takagi, Masahiro ^d  

^a OSAKA UNIVERSITY   (Japan)

^b Mahidol University ^*  (Thailand)

^c KYOTO UNIVERSITY   (Japan)

^d JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Bacterial expression; Hepatocellular carcinoma (HCC); Inclusion body; Single chain Fv (scFv)

Indexed keywords

GENOMIC DNA; GLYCINE; HISTIDINE; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; METAL ION; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY HEP27; SERINE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIBODY COMBINING SITE; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER CELL CULTURE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DNA RECOMBINATION; DRUG BINDING SITE; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; GENETIC CODE; HUMAN; HUMAN CELL; IMMUNOGLOBULIN VARIABLE REGION; LIVER CELL CARCINOMA; MOLECULAR WEIGHT; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SEQUENCE TAGGED SITE;

BACTERIA (MICROORGANISMS); MURINAE;

EID: 0037808989     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-2-16     Document Type: Article

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